氨对胞内贮存物短程反硝化N2O产生量的影响赵日祥

40EnvironmentalScience&Technology4012201712Vol.40No.12Dec.2017.N2O[J].2017401238-43.ZhaoRixiang,ZhaoJianqiang,DingXiaoqian,etal.Effectofammionaonnitrousoxideemissionduringdenitrificationofnitritewithintracellularstorageaselectronicdonors[J].EnvironmentalScience&Technology2017401238-43.N2O*,710064N2O//A/O/ASBR/An/A,4N2O。COD200mg/L0、40、60、80mg/L40mg/L。N2O。。NO2--N0N2ON2O。X703.1Adoi10.3969/j.issn.1003-6504.2017.12.0071003-6504(2017)12-0038-06EffectofAmmionaonNitrousOxideEmissionDuringDenitrificationofNitritewithIntracellularStorageasElectronicDonorsZHAORixiang,ZHAOJianqiang*,DINGXiaoqian,GAOKun,MIWeixing,LIANGXueSchoolofEnvironmentalScienceandEngineering,Chang'anUniversity,Xi′an710064,ChinaAbstractTheeffectofammoniaonnitrousoxide(N2O)emissionduringdenitrificationofnitriteunderanoxicconditionwithintracellularstorageaselectrondonorswereexaminedbyusingstable-operatedsequencingbatchreactor(SBR)whichisoperatedinthestablemodeofanaerobic/aerobic/anoxic(A/O/A)andregularlychangingtothemodeofanaerobic/anoxic(An/A).Theeffectofammoniaunder4differentconcentrationsonN2Oemissionwasinvestigatedbyusingthemicroorganisms′characteristicthatthelowmolecularweightorganiccompoundtranslatedintointracellularstoragewhileaddingnitritetothereactorattheinitialstageofanoxicphase.Theconcentrationofreactorfedwithsyntheticwastewatercomposedof200mg/LCODundertheinitialstageofanaerobicphase,andduringtheinitialstageofanoxicphase,thereactorwasadded40mg/Lofnitratewith0,40,60,80mg/Lofammonia,respectively.ItturnedoutthatthedenitrificationdurationandtheN2Oemissionwerepromotedwiththeincreaseofammonia′sconcerntration.Thereasonmaybethatthefreeammoniahasamodestinhibitoryontheactivityofdenitrification'microbe.Withtheincreaseoftheconcentrationofammonianitrogen,thefreeammoniaaswellastheinhibitionwasincreased.Atthesametime,whentheconcentrationofNO2--Nwascloseto0,theN2O′sreductionbasedonintracellularstoragewasabletotakeplace,whichshowedthatnitriteinhibitedtheN2O′sreduction,andtheinhibitionconcentrationwasverylow.Keywordsdenitrification;ammonia;nitrousoxide;nitrite;inhibition《》@vip.126.com2017-02-172017－04－192014K15-03-02(1989-)[e-mail]*[e-mail]。N2O3CO2、N2O、CH4CO2310110[1]。N2O1220047.9%[2]。N2O8%0.2%~0.3%[3]。N2O0.3~3×109t/aN2O2.5%~25%[4]。N2O。N2O。。→→→→。NosN2O[5]。N2O[6]、[7]、pH、[8]、[9]。。FA-FreeAmmoniaFA。Nara[10]Waki[11]Ahn[12]N2O。Wu[13]N2O。VadiveluFA[14]。N2OSBR/An/A,4N2O。11.11(se－quencingbatchreactorSBR)35cm16cm6L5L(30±1.0)℃。A/O/A38h。5min7.5h1h2h4.5h、25min。2LKG316T。A/O/AA/O/A。1.2A/O/AC6H12O60.21g/L,KH2PO40.02g/L,MgSO4·7H2O0.02g/L,CaCl20.0064g/L,NaHCO30.6g/L,NH4HCO30.271g/L,0.04mL/L[15]。An/A40、40、60、80mg/LC6H12O60.21g/LKH2PO40.02g/LMgSO4·7H2O0.02g/LCaCl20.0064g/LNaHCO30.6g/L0.04mL/LNaNO20.20g/L。。MLSS2800mg/L。FA5mg/L[1617]。0.5mg/L[18]20d99%。16MLSS3800mg/LMLVSS3116mg/L。A/O/A1dAn/A。4N2O。1.3An/AA/O/A330℃5min、3N2O40An/A4COD200mg/L1h30mg/L。Qin“”“”[21]。COD[22]。30min。24tAtBtCtD。2NH4+NH3,NH3+H2O→←NH4++OH-[23]ρ(FA)=1714×ρNH4+-N×10pHexp6334/273+←←ttt+10pH11:ρ(FA)mg/Lρρ(NH4+-N)/(mg·L-1)ρ(NO2--N)/(mg·L-1)ρ(NO3--N)/(mg·L-1)ρ(N2O)/(mg·L-1)NO2--N/min/minA041.210.111.21180310B40.5941.250.513.66280370C59.2139.860.314.05315415D79.7240.260.215.0933042014Table1Theinitialconcentrationofnitrogencompoundsandreactiontimeofthefourexperiments。1mVNaNO2NH4HCO31h。1.41.4.1pHNO3--N、NO2--N、CODNH4+-N[19]pHpHS10Hach-HQ30dHACH。1.4.2N2ON2OUnisense。N2ON2O[20]。22.11。2.2COD12N2ONH4+-N)mg/L;t℃。4FA。FA,[23]。(Nir)、(Nor)(Nos)。2.3NO2--NN2O-N3A、B、C、DNO2--N0mg/LN2ON2O。Zhou[24]NO2--NN2O。NO2--NN2O0mg/L。3、、[25]。、、[26]。NO2--N0.2mg/LN2O0.2mg/LN2O。E=乙0.002·Cx(N2O)dt22EN2Oemission0.002N2OCx(N2O)N2Ot。N2ON2O。N2ON2O[27]。3(b、c、d)3N2O35mg/L3a。N2O。2.4pH44pH3pH8.3pH8.65pH。N2OEMPpH[28]。3１An/ASBR40N2O。２N2O0.2mg/L。３N2OEMPpH。[][1]IPCC.ClimateChange2007:SynthesisReport.ContributionofWorkingGroupsⅠ,ⅡandⅢtotheFourthAssessmentReportoftheIntergovernmentalPanelonClimateChange[C].Geneva,Switzerland:IPCC.[2]IPCC2006,2006IPCCGuidelinesforNationalGreenhouseGasInventories,PreparedbytheNationalGreenhouseGasInventoriesProgramme[R].PublishedbyIGES,Japan.[3]KhalilMAK,RasmussenRA.Theglobalsourcesofnitrousoxide[J].JournalofGeophysicalResearch-Atmospheres(1984-2012),1992,97(D13):14651-14660.[4],,,.SBR[J].,2009,29(3):312-317.LiLingyun,PengYongzhen,YangQing,etal.Factorsopti－mizationofrapidstartupforpartialnitrificationinSBRpro－cess[J].ChinaEnvironmentalScience,2009,29(3):312-317.[5]LuH,ChandranK.Factorspromotingemissionsofnitrousoxideandnitricoxidefromdenitrifyingsequencingbatchre－actorsoperatedwithmethanolandethanolaselectrondonors[J].BiotechnologyandBioengineering,2010,106(3):390-398.[6]DruryCF,FindlayWI,McKenneyDJ.Oxygeninhibitionofdenitrificationinchloroformfumigatedandnon-fumigatedsoil[J].SoilBiologyandBiochemistry,1991,23(8):711-715.[7],,.N2O[J].,2012,38(6):898-903.WangShuying,WangSai,GongYoukui.Effectofρ(COD)/ρ(N)onnitrousoxide(N2O)productioninbiologicaldenitrifi－cation[J].JournalofBeijingPolytechnicUniversity,2012,38(6):898-903.[8],,,.COD/NpHN2O[J].,2014(8):2003-2009.LiPengzh