蛋白质在城市污水活性污泥处理系统中的降解动力学模型研究黄满红

28　32009　　5　　　ENVIRONMENTALCHEMISTRYVol.28,No.3May　　2009　2008721.　＊(No.50138010,50578114);(No.113-10-0044018);(No.B604);;(DERC)(No.2005RD001).＊＊.＊黄满红1,2　李咏梅2＊＊　顾国维2(1　,,201620;2　,,,200092)　　--,、,、,.,Freundlich、,.,K、K0.400.98,,.(10%).、,.　,A/A/O,,.　　--(A/A/O)[1],,A/A/O、A/A/O.[2—4],.,50%—60%,.　　--,,A/A/O,GFC,.1　　　,CODCr204—440mg·l-1,TOC40—138mg·l-1,TN50—70mg·l-1,TP4.5—6.5mg·l-1,pH7.0—8.0.,1.1　Table1　Componentofthesyntheticwastewater/mg·l-1/mg·l-1NaHCO3125.00FeCl3·6H2O0.0937KH2PO424.00H3BO30.0094NH4Cl100.00CuSO4·5H2O0.0019180KI0.0112KCl4.17MnCl2·4H2O0.0075MgSO4·7H2O27.50ZnSO4·7H2O0.0075CaCl22.50EDTA0.6248　3:405　　　　(A/O).25℃,15d,,MLSS3000mg·l-1.(SRT)15d,100%,//1∶1∶3.　　0—0.20mg·l-1,0.25—2.10mg·l-1,6.45—8.13mg·l-1.　　、1L,125r·min-1,,3500r·min-115min,,.2　　　、FreundlichLangmuir[5](2),3.3,,Langmuir,2,Freundlich.2　(mg·l-1)Table2　Proteinconcentrationintheaqueousphaseunderadsorptionequilibriumconditions(mg·l-1)15.008.517.895.5225.0013.3111.249.3550.0026.7923.6820.68100.0053.6547.3843.35150.0084.0072.2362.89200.00105.7196.7189.783　Table3　AdsorptionisothermsofproteinsontothesludgeR2Kp=0.41110.9915Langmuirqm=19.9158,a=-4.45×10440.5732Freundlichkf=0.3557,n=0.96840.9918Kp=0.49080.9988Langmuirqm=22.0616,a=-2.6425×10460.4568Freundlichkf=0.5798,n=1.03150.9992Kp=0.58530.9889Langmuirqm=23.3129,a=-2.5345×10460.3478Freundlichkf=0.9544,n=1.13060.9954:Kp,qmaLangmuir,kfnFreundlich.　　,、Kp,、,;Freundlich,,.3　　　、,TOC53mg·l-1.2892mg·l-1.1.1,10min,,,10min.,10min1h,406　　　　　　　　28,...1　Fig.1　Degradationofproteinsandaminoacids4　　　A/A/O、、1L,31L,,,.125r·min-1,.　　2(ASM2)[6],,(1)、(2)(3)A/A/O:rb()=ηFeKbSSKS+SSX(1)rb()=ηNO3KbSSKS+SSX(2)rb()=KbSSKS+SSX(3),Kb,KS,ηFeηNO3,XVSS,SS.　　,,(4).dS/dt=(S-Se)/t(4)XtS0-Se=-KSKbSe+1Kb(5),S0,Se,t.x=1/Se,y=Xt/(S0-Se),Km=-(KS/Kb),m=1/Kb,(5)(6):y=Kmx+m(6)　　、4.4　Table4　Degradationkineticparametersofproteins:y=Kmx+mR2Kb/mg·g-1VSS·h-1KS/mg·l-1ηy=3.5336x+452.190.96352.217.810.40y=1.9213x+185.090.93695.4010.380.98y=1.9391x+155.660.97276.4212.461　　4,、Kb,KbKS、.、η0.40,0.981.00,ASM2　3:407　　(ηFeηNO30.10.6).,A/A/O.　　A/A/O,5.5,,10%.5　Table5　　Differencebetweenpredictionandmeasurement/mg·l-1/mg·l-1/mg·l-1/%35.1932.762.436.8917.2815.601.689.724.284.430.143.355　　　(GFC).、,2120min、GFC,2,,、.,1000—2000020000—670000,1000—2000020000—670000.、,10min,7h,,.,.(400),,.2　(A)、(B)(C)120minGFCFig.2　GFCchromatographofeffluentsfromanaerobic(A),anoxic(B)andaerobic(C)reactorsafter120mindegradation　　,Freundlich、.,10%,.、,、,,(400).　　　[1]　KoichiS,ShinyaM,SatoshiOetal.,ModelingandExperimentalStudyontheAnaerobic/Aerobic/AnoxicProcessforSimultaneousNitrogenandPhosphorusRemoval:TheEffectofAcetateAddition.ProcessBiochemistry,2008,43∶605—614[2]　BaezaJA,GabrielD,LafuenteJ,EffectofInternalRecycleontheNitrogenRemovalEfficiencyofanAnaerobic/Anoxic/Oxic(A2O)WastewaterTreatmentPlant(WWTP).ProcessBiochemistry,2004,39∶1615—1624408　　　　　　　　28[3]　RodrigoMA,SecoA,Penya-rojaJMetal.,InfluenceofSludgeAgeonEnhancedPhosphorusRemovalinBiologicalSystems.Wat.Sci.Tech.,1996,34∶41—48[4]　BarkerPS,DoldPL,CODandNitrogenMassBalancesinActivatedSludgeSystems.Wat.Res.,1994,29∶633—643[5]　,,,(Ⅱ).,2006,25∶414—419[6]　MogensH,CharacterizationofWastewaterforModelingofActivatedSludgeProcess.Wat.Sci.Tech.,1992,25∶1—15DEGRADATIONKINETICMODELOFPROTEINSINACTIVATEDSLUDGETREATMENTSYSTEMSFORMUNICIPALWASTEWATERHUANGMan-hong1,2　　LIYong-mei2　　GUGuo-wei2(1　CollegeofEnvironmentalScienceandEngineering,DonghuaUniversity,Shanghai,201620,China;2　StateKeyLaboratoryofPollutionControlandResourceReuse,TongjiUniversity,Shanghai,200092,China)ABSTRACT　　Theremovalcharacteristicsbyadsorptionanddegradationofproteinswerestudiedinanaerobic/anoxic/oxic(A/A/O)municipalwastewatertreatmentsystems.Theadsorptionisothermsandthedegradationkineticsofproteinsbythesludgeunderanaerobic,anoxicandaerobicconditionswereseparatelydetermined.ThedegradationmodelparameterswerecalibratedbytheexperimentaldataobtainedfromtheperformanceofA/A/Osystem.Theresultsshowedthattheadsorptionofproteinsontothethreekindsofsludgecouldbeade-quatelydescribedbyFreundlichmodel.Hydrolysisratewasthekeystepinthedegradationprocessofpro-teins.Theratiosofproteindegradationrateunderanaerobicandanoxicconditionstothatunderoxicconditionswere0.4and0.98,respectively,indicatingthatproteinwaseffectivelydegradedunderanaerobicandanoxicconditions.Themodelwasusefulinforecastingtheeffluentqualityofanaerobic,anoxicandoxictanksintheA/A/Osystem,andtherelativeerrorwaslessthan10%.Theaccumulationofacid-solubleproteinwasnotobsevedintheanaerobic,anoxicandoxictanks.　　Keywords:protein,A/A/Omunicipalwastewatertreatmentsystem,adsorption,degradationkinetics.