ELISA和GICA在食源性致病菌检测中的应用

[](1962-),,,,ELISAGICA1,1,2,3,4,1(11,510070;21,430071;31,100049;41,510070)[]ELISA;GICA;;[]R446161[]A[]1004-8685(2007)03-0566-03,,,,39162%[1],,(PCR),(ELISA)(GICA)ELISAGICA,,,,,[2]ELISAGICA,(Salmonel2la)O157(EscherichiacoliO157)(Listeriamonocytogenes)B1M1(AflatoxinB1&AflatoxinM1,AFB&AFM)1ELISAELISA2070,EngvallVanWeemen(radioim2munoassay,RIA)ELISA,,,ELISA3:,3113ELISA111,,[3]ELISA350,100%9917%[4]ELISA,9414%9717%[5]ELISAA-F106cfu/LELISA,,ELISAMansfieldLP[6]ELISA2103cfu/25g,27h,18h6h;7296h112O157O157,[7]O157:H7,ELISA103104cfu/ml;O157,O157;,O157011cfu/g(ml)NorvalJC[8],3h103cfu/mlO157:H7SongJM[9]ELISA,3cfu/ml,,,PCRELISA,PCR-66520073173ChineseJournalofHealthLaboratoryTechnology,Mar2007;Vol17No3©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[10]O157:H7PCR-ELISA01110cfu;105cfu/g6h,113AFBAFM,[11]ELISAB110pg/ml,2006,SunXL[12]AFBAFBAFB,10min,215ng/ml[13]M1,AFM,MihaelaB[14]AFM,11pg/ml114L1monocytogenes,,1999,EnnedeBoer[15]ELISAL1monocytogenes,103105cfu/ml,1624h,SewelldAM[16]L1monocytogenes,104105cfu/ml,52h,22070,FaulkTaylor,,,,,GICA,[17]A,85%,214107cfu/ml,100%[18],,,211106cfu/ml,,3733d,[19]O157:H7,215%311106cfu/ml[20]O_9,O_94105cfu/;2105cfu/,[21],O_9,10min6104cfu/,O_9,[22]EV76214105cfu/,,10min3,ELISA,,,,;,ELISA,,,,;,,106cfu/L[4],ELISA,,,,,6104cfu/ml[23],,,,,,,,[][1]1[N]1,2004-11-241[2]1[J]1,2005,14(19):2609-26111[3],1ELISA76520073173ChineseJournalofHealthLaboratoryTechnology,Mar2007;Vol17No3©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[J]1,1998,26(5):32-331[4],,1[J]1,2002,19(7):25-281[5],,,1[J]1,1997,7(3):136-1391[6]MansfieldLP,ForsytheSJ1ThedetectionofSalmonellaserovarsfromanimalfeedandrawchickenusingacombinedimmunomagneticsepa2rationandELISAmethod[J]1FoodMicrobiology,2001,18(4):361-3661[7],1O157ELISA[J]1,2003,32(6):606-6091[8]NorvalJCStrachan,IainDO1AsensitivemicrospherecoagulationELISAforEscherichiacoliO157:H7usingRussellsvipervenom[J].FEMSMicrobiologyLetters,2000,186(1):79-841[9]SongJM,TuanVo-Dinh1MiniaturizedbiochipsystemfordetectionofE1coliO157:H7basedonantibody-immobilizedcapillaryreactorsandenzyme-linkedimmunoassay[J]1AnalyticaChimicaActa,2004,507(1):115-1211[10]GeBeilei,ZhaoShaohua,HallR,etal1APCR-ELISAfordetec2tingshigatoxin-producingEscherichiacoli[J]1MicrobesandInfec2tion,2002,4(3):285-2901[11]1ELISAAFB_1[J]1,2001,11(3):335-3361[12]SunXiulan,ZhaoXiaolian,TangJian,etal1Developmentofanim2munochromatographicassayfordetectionofaflatoxinB[1]infoods[J]1FoodControl,2006,17(4):256-2621[13]1M_1[J]1,2004,5(5):60-611[14]MihaelaB,LauraM,MariaCM,etal1AflatoxinM1determinationinrawmilkusingaflow-injectionimmunoassaysystem[J]1AnalyticaChimicaActa,2004,520(1-2):141-1481[15]EnnedeBoer,RijkeltRBeumer1Methodologyfordetectionandtyp2ingoffoodbornemicroorganisms[J]1IntJFoodMicrobiol,1999,50(1):119-1301[16]SewellAM,WarburtonDW,BovilleA,etal1Thedevelopmentofanefficientandrapidenzymelinkedfluorescentassaymethodforthede2tectionofListeriaspp1fromfoods[J]1IntJFoodMicrobiol,2003,81(2):123-1291[17],,,1[J]1,2001,29(4):45-471[18],,,1[J]1,2004,32(4):36-381[19],,,1O157:H7[J]1,2005,21(6):705-7061[20],,1O_9[J]1,2005,15(3):30-321[21],,,1O_9[J]1,2005,21(3):210-2121[22],,,1[J]1,2005,20(1):1-31(:2006-11-21)(501)221111(cfu/)(%)100113104781621009121036713410021410482169100715103721461005161048318121222(%)(%)(%)100301403112111410022174214301801003415671062150100161441156010036128715411403,,67134%83181%,16144%36128%,1156%7154%,0%215%12,25,715103(cfu/),516104(cfu/),76198%,28108%,4134%,1117%,,,,,-,,,,,,,,[][1],,,1[J]1,1996,6(2):1241[2],,,1[J]1,2004,14(5):6161[3]1[J]1,2000,15(1):321(:2006-10-31)86520073173ChineseJournalofHealthLaboratoryTechnology,Mar2007;Vol17No3©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.