ELISA法检测红曲中的黄曲霉毒素B1

23420047JournalofWuxiUniversityofLightIndustryVol.23No.4Jul.2004:10092038X(2004)0420010203:2003210223;:2003212206.:(02EFN213).:(19742),,,,.ELISAB11,1,12,2(1.,214063;2.,350500):B1,/(11),ELISA,,.2.5,5,10g/kg,101%146.8%,,13%,2.5g/kg.:;;ELISA;B1:TS201.6:ADeterminationofAflatoxinB1inRedLeavenbyELISAGONGYan1,CAIJian2rong1,ZHAOXiao2lian1,HUANGGuo2shui2,SUNKai2xi2(1.JiangsuInstituteofMicrobiology,Wuxi214063,China;2.HealthandEpidemicPreventionStationofLiangjiangCity,Liangjiang350500,China)Abstract:TheextractionmethodofaflatoxinB1inredleavenwasstudiedinthispaper.Theresultsshowedthat10mLmethanolandwatersolutionoftotal25mLextractextractedbychloroformcaneliminatenegativeerrorandimprovethedetectionsensitivity.TherecoveriesofaflatoxinwhenB1spikedat2.510g/kgcouldbeattherangeof101%146.8%andtherelativedeviationislessthan13%.Thedetectionlowlimitwas2.5g/kg.Keywords:redleaven;extractionmethod;ELISA;AFB1[1,2],,,.,,,.,,(AFB1).,,(Monascus).B1,AFB1.,AFB1[3],ELISA.11.1:MK3,;:MS2,IKA;:;:HH2S;:AB2042S,10mg210g,;:;:(100mL),(125mL),(50mL),(25mL),(10mL),.1.2B1ELISA:;:,;:,;Na2SO4:;PBS(pH7.4):Na2HPO43.0g,NaH2PO40.25g,NaCl8.7g,1L.1.3.():2.5g100mL,25mL/(11),15min,,1/4,.():2.5g100mL,25mL/(11),15min,,.10mL(1g)125mL,20mL,5min,(30min),,5gNa2SO450mL,10mL,,,,.,65,.5mL,10mL,5mL,,,10mL,.1.4AFB1AFB11mg/mL,/PBS(14)10g/mL,(361nm),:=AM1000f/E:AFB1(g/mL);A;MAFB1312;E21800;f(1).10g/mL,/PBS(0.1,0.5,1,5,10g/kg).1.5ELISAB1ELISA,.1.5mL(D)(C),,.2,.50LAFB1,50LAFB1(,AFB1),50L,50L,,3730min.,5,.50L50L,3715min.50L,450nmA.22.1AFB1(0,0.1,0.5,1,5,10g/kg),,,Ai/Ao,(1).r=0.9926,y=-0.9415x+8123,0.15g/kg.1Fig.1StandardcurveforAFB1quantitatineanalysis2.2,AFB1,0.5,1,2.5,5,10g/kg,,AFB1,,,,114:ELISAB11.1Tab.1TherecoveriesofAFB1spikedinsampleAFB1/(g/kg)AFB1/(g/kg)/(g/kg)/%0.517.51648.0118.88461.014.272.522.03310.4523.93193.21019.80198.00.58.53258.019.05181.07.242.510.91146.8513.15118.21017.35101.1=AFB1-AFB1AFB1100%:AFB1,,,AFB1.1,2.5g/kg.2.31#,2#,3#,,3AFB1(n=10),,2.2,ELISAAFB1,13%,B1ELISA.2Tab.2Resultsofrepetibility/(g/kg)/(g/kg)/%1#7.367.117.337.257.197.187.287.137.057.387.220.1312.052#5.455.335.495.305.515.255.285.395.415.555.430.1312.583#12.8213.0112.7512.9012.8712.9312.6912.6412.8912.7012.820.1211.4331)ELISA[4],,,,,,,.AFB1[5],,.2)[6],/AFB1,,,.,,,.3)AFB1,[7]()5g/kg.,AFB1,2.5g/kg,,.:[1].[J].,2002,(1):22-23.[2].[J].,2001,(5):5-6.[3].[M].:,1996.137-149.[4],,.ELISAB1[J].,2003,(4):82-84.[5],,.[M].:,2001.185-194.[6],,.[J].,1999,(5):26-27.[7]GB2761281,B1[S].(:)2123