日粮纤维对猪回肠末端氨基酸及其内源性氨基酸流量的影响

,,,(,410125):S828.5:A:036626964(2004)0420473204,,,,,,,,,;,,,,2()()11.1530kg,,T[1]5(:(A),4%(B)8%(C)12%(D)8%(E)[2],1.2,,[3]:,:2003203207::(19672),,,,,,E2mail:[e-mail],,pH10.3;44dpH10.3;,pH4.5,4(4000römin)10min,(pH4.5),,,3;,50%,,(Sigma),100mgökg1.3559d,7,24h,;9,(8:00),24h[4],SPSS10.0,,Duncan1.4AAflow=[AA]digesta([Marker]dietö[Marker]digesta)AAflow,[AA]digesta,[Marker]diet[Marker]digesta(7d)(9d)TDlys=([HA]diet-[HA]flow)ö[HA]diet100TDlys,[HA]diet,[HA]flowLYSeflow=LYSflow-[LYS]diet(1-TDlys0.01)LYSeflow,LYSflow,[LYS]diet,TDlys,2004,35(4),4732476ActaVeterinariaetZootechnicaSinicaEndAAflow=[AAliteratureöLYSliterature]LYSeflowEndAAflow(),AAliteratureöLYSliterature([5],[6]),LYSeflow22.11,D,6.00gökgDMI;,,(A),8%(E)4%(B)8%(C),,(P0.05);12%(D),(P0.05)8%4%8%,,(P0.05);12%,(P0.05)3,,();,4%8%,;12%,1.00gökgDMI0.26gökgDMI(P0.05),4,(P0.05),,,1(gökg)Table1ThetotalNflowandtheflowofeachaminoacidinilealdigestagökgDMIItemADietABDietBCDietCDDietDEDietEIlealTN33.960.43a5.001.13a5.740.81a6.000.94b4.000.35aLys0.850.16a1.610.43ab1.750.27ab1.570.33b0.950.11aMet0.320.05a0.390.13ab0.410.07ab0.460.08b0.310.03aCys0.410.06a0.490.11a0.570.15ab0.690.14b0.420.07aThr1.220.13a1.450.37ab1.690.26ab1.850.32b1.250.15aTrp0.310.05a0.370.11ab0.400.08ab0.480.09b0.340.03aArg0.890.12a1.020.27ab1.250.23ab1.480.21b0.900.13aIle7.411.20a9.182.74ab9.462.32ab11.852.09b7.671.34aLeu1.410.15a1.800.59ab1.830.37ab2.180.41b1.460.19aVal1.040.13a1.320.45ab1.340.28ab1.640.31b1.060.14aHis0.440.05a0.550.16ab0.580.11ab0.690.14b0.460.04aPhe1.040.11a1.320.40ab1.390.24ab1.630.29b1.100.16aGly1.200.20a1.490.35a1.650.41a2.220.39b1.350.26aSer1.010.15a1.170.26ab1.280.27ab1.480.28b1.020.15aPro1.320.15a1.600.28ab1.790.35bc2.230.36c1.460.36abAla1.100.16a1.350.45ab1.390.26ab1.720.28b1.080.14aAsp2.030.33a2.370.63ab2.430.52ab2.900.58b2.080.34aGlu2.490.31a3.550.97a3.670.92ab4.890.95b2.690.54aMSD,(P0.05)3ThedataintableareMSD,andthedatawithdifferentsmalllettersinthesamerowarediffersignificantly(P0.05).Thesamebelow.3ThetotalNinilealdigesta.2.22,D,5.69gökgDMI,,;,,,8%4%8%(P0.05);12%,(P0.05)47435,8%4%8%,(P0.05),12%,(P0.05)3,8%,4%,12%,,(P0.05)(0.651.30gökgDMI)(0.490.52gökgDMI0.591.43gökgDMI0.440.86gökgDMI)[68];(0.23gökgDMI0.24gökgDMI0.22gökgDMI)[6,9,10],,,2(gökg)Table2TheendogenousNflowandendogenousflowofeachaminoacidattheterminalileumgökgDMIItemADietABDietBCDietCDDietDEDietEEndN32.780.66a4.541.76ab4.481.26ab5.691.53b3.200.43aLys0.650.14a1.020.37ab0.940.27ab1.300.32b0.700.09aMet0.200.04a0.300.10ab0.310.07ab0.410.08b0.230.03aCys0.290.07a0.480.20ab0.440.14ab0.580.17b0.330.05aThr0.930.21a1.520.57ab1.610.41ab2.080.49b1.000.14aTrp0.290.05a0.420.13ab0.440.09ab0.680.11b0.320.03aArg0.530.14a0.890.36ab0.820.26ab1.070.31b0.620.09aIle0.510.13a0.870.35ab0.800.25ab1.040.31b0.610.09aLeu0.670.17a1.120.46ab1.030.33ab1.350.40b0.780.11aVal0.740.19a1.240.50ab1.140.36ab1.480.44b0.860.12aHis0.260.07a0.440.18ab0.400.13ab0.520.15b0.300.04aPhe0.460.12a0.780.32ab0.710.23ab0.930.27b0.540.08aGly1.250.32a2.110.86ab1.930.61ab2.520.74b1.470.21aSer0.930.24a1.570.63ab1.430.45ab1.870.55b1.080.16aPro1.550.40a2.621.06ab2.390.78ab3.130.92b1.820.26aAla0.680.17a1.150.47ab1.050.33ab1.370.40b0.800.11aAsp1.120.29a1.880.76ab1.720.55ab2.250.66b1.310.19aGlu1.930.44a3.111.18ab2.970.84ab4.001.03b2.080.29a3EndogenousNattheterminalileum.2.33,,8%,4%8%12%,(P0.05),;,8%8%12%,(P0.05),4%3.34%,(P0.05)3,,(P0.05)3Table3Thetruedigestibilityoflysineandthetruedigestibilityofproteinattheterminalileum%ItemADietABDietBCDietCDDietDEDietELys94.031.0695.550.9896.000.6194.670.9395.320.97CP94.082.32a97.422.70b95.251.86ab96.283.76ab95.980.74ab3,,,,,5744::[1].2T[J].,1989,5:8185.[2],,,.[J].,2003,34(3):239245.[3]MagaJA.Measurementofavailablelysineusingtheguanidinationreaction[J].JFoodSci,1981.46:132134.[4]deLangeCFM,SauerWC,MosenthinR,etal.Theeffectoffeedingdifferentprotein2freedietsontherecoveryandaminoacidcompositionofendogenousproteincollectedfromthedistalileumandfecesinpigs[J].JAnimSci,1989,67:746754.[5]BoisenS,MoughanPJ.Dietaryinfluencesonendoge2nousilealproteinandaminoacidlossinthepig2are2view[J].ActaAgricScandSectAnimSci,1996,46:154164.[6]deLangeCFM,SouffrantWB,SauerWC.Realilealproteinandaminoaciddigestibilitiesinfeedstuffsforgrowingpigsasdeterminedwiththe15N2isotopedilutiontechnique[J].JAnimSci,1990,68:409418.[7]MosenthinR,SauerWC,AhrensF.Dietarypectin’seffectonilealandfecalaminoaciddigestibilityandex2ocrinepancreaticsecretionsingrowingpig[J].JNu2tr,1994,124:12221229.[8]NyachotiCM,deLangeCFM,SchulzeH.Estimat2ingendogenousaminoacidflowsattheterminalileumandtrueilealaminoaciddigestibilitiesinfeedstuffsforgrowingpigsusingthehomoargininemethod[J].JAnimSci,1997,75:32063213.[9]CaineWR.Ilealrecoveryofendogenousaminoacidsinpigs[D].Wageningen,Netherlands:Departmentofanimalnutrition,Wageningeninstituteofanimalsci2ences,Wageningenagriculturaluniversity,1997.[10]ButtsCA,MoughanPJ,andSmithWC.Endoge2nousaminoacidflowattheterminalileumoftheratdeterminedunderconditionsofpeptidealimentation[J].JSciFoodAgric,1991,55:175187.DietaryCellulose’sEffectontheFlowo