喷雾干燥法制备蛋氨酸微胶囊工艺的优化

第29卷第5期2010年9月食品与生物技术学报JournalofFoodScienceandBiotechnologyVol.29No.5Sept2010:1673-1689(2010)05-0681-06:2009-11-3:(BA2007089):(1978-),,,,Email:[e-mail]*:(1953-),,,,,Email:[e-mail]喷雾干燥法制备蛋氨酸微胶囊工艺的优化牛化欣1,2,过世东*1,谢中国1,祝爱侠1(1.江南大学食品学院,江苏无锡214122;2.内蒙古民族大学动物科技学院,通辽028000):为了制备出在对虾配合饲料里能被对虾利用的微胶囊蛋氨酸,选用明胶和海藻酸钠作微胶囊壁材,对喷雾干燥法制备晶体蛋氨酸微胶囊的工艺优化进行了研究,采用正交试验法确定了微胶囊的最佳配方和喷雾干燥的最佳工艺参数结果表明,晶体蛋氨酸微胶囊的优化配方:壁材为海藻酸钠质量分数1%和明胶质量分数1%芯材为蛋氨酸质量分数15%进料温度65;喷雾干燥最佳工艺条件为:进风温度195出风温度80进料速度45mL/min,在此条件下,晶体蛋氨酸微胶囊包埋率为89.6%,用扫描电镜(SEM)观察微胶囊表面光滑圆整无小孔,形状规则,无粘连现象:喷雾干燥;晶体蛋氨酸;微胶囊;工艺参数;优化:S963.73:AOptimizationofTechnologyforMicroencapsulationofMethioninebySpray-dryingNIUHua-xin1,2,GUOSh-idong*1,YUANXin-hua1,XIEZhong-guo1,ZHUA-ixia1(1.SchoolofFoodScienceandTechnology,JiangnanUniversity,Wuxi214122,China;2.SchoolofAnimalSc-ienceandTechnology,InnerMonglliaUniversityfortheNationalties,Tongliao028000,China)Abstract:Inordertopreparemicroencapsulationmethionine,whichcouldbeabsorbedandutilizedinshrimpfeeds,thewallmaterialsandthetechnologyofembeddingbyspray-dryingwerestudiedinthismanuscript.Themethodwasconfirmed,whichusedthealginateandgelatinaswallmaterials,themethioninepowderascorematerialandspraydryingtechnology.Resultsshowedthatthebestparametersoftheembeddingofthemicrocapsulewerethatthe1%alginateand1%gelatin,themethioninepowderwas15%,thefeedtemperaturewas65,theairinlettemperaturewas195andtheairoutlettemperaturewas80,thefeedspeedwas45mL/min,andtheembeddingratereachedto89.6%.ScanningElectronMicroscopy(SEM)hasbeenappliedtothestudyonthesurfacesuperstructureofmicroencapsulatedmethionine,whichwasofregulationanduniformity,notconglutination.Keywords:spray-drying,crystallinemethionine,microcapsulation,technologyparameter,optimization,,,,,,,[1][2][3],[4],[5][6],[7],,[8-9][10][11],,1材料与方法11DL-,,985%,;,12QZ-5:;FW100:;NDL-:;APV1000:APV;:Agilent1100;(SEM)Quanta-200:FEI13131原料预处理(60m)132微胶囊复合壁材配比,,,123g/dL1g/dL254565,133晶体蛋氨酸微胶囊制备,,30min,30MPa,,:134微胶囊配方正交试验设计1g/dL(1),04MPa,190,80,,,1Tab.1Factorsandcodedlevelsoftheorthogonalexperimentsonmicroencapsulationformula/%/%/11525221545332565682食品与生物技术学报第29卷135晶体蛋氨酸微胶囊化工艺条件参数优化试验,[7],(2),2Tab.2Theorthogonalexperimentsonmicroencapsulationtechnologyparameter///mL/min11656515218080303195954514141粘度的测定,254565142乳化稳定性的测定30MPa,50mL50mL,2545652h:(%)=-100%143微胶囊化效果可用包埋率来衡量:(%)=100%5g50mL,,,65,70mg100mL,;30min,;15,,20kV,,2结果与讨论21,,,,(pH09~15),[12],,(pH15),(pH65),,,,31%Tab.3Viscosityandstabilityof1%sodiumalginateanddifferentlevelgelatinsolution//(g/dL)001020304050/MPas001020304050/%25247.5295.6366.5734.91208.61664.081899293949545152.5203.6271.0529.6874.51268.572868990929465103.0126.2159.6367.1519.7738.06682878890933,,,,,683第5期牛化欣等:喷雾干燥法制备蛋氨酸微胶囊工艺的优化,,,,,,,,65,3g/dL+1%3671MPas1g/dL1%2%3%2265,65,18080,454Tab.4ResultsoforthogonalexperimentA/%B/%C/D()/%1111172.72122287.53133386.94212370.45223188.26231281.77313266.48321375.99332178.6182.3769.8376.7779.83280.1083.8778.8378.53373.6382.4080.5077.738.73.14.033.732.105Tab.5VarianceanalysisoforthogonalexperimentABC1232335701209967422226161178501049337F18285297311P005190018502431()(P005),(P005),A1B2C31%1%15%652.322,1%1%15%65,,67684食品与生物技术学报第29卷6Tab.6ResultsoforthogonalexperimentA/B/C/(mL/min)D()/%1111185.62122289.83133377.54212381.25223184.76231268.67313271.38321372.99332166.5170.2379.3776.5777.83278.1782.4777.2075.70384.3070.8778.9379.1714.0711.602.373.477Tab.7VarianceanalysisoforthogonalexperimentA/%B/%C/29843216.4218359012222149.2110821917450F16271180049P005790078106707(P005),A3B2C31958045mL/min242223,1%1%15%651958045mL/min3,904%,8885%,8963%,,25SEMSEM10~60m,,,215~60m,,,,1Fig.1SEMphotoofcrystallinemethionine2Fig.2SEMphotosofmicroencapsulationmethionine685第5期牛化欣等:喷雾干燥法制备蛋氨酸微胶囊工艺的优化3结语:1%1%15%651958045mL/min896%SEM,,,,,,(References):[1]OnoderaR.Methionineandlysinemetabolismintherumenandthepossibleeffectsoftheirmetabolitesonthenutritionandphysiologyofruminants[J].AminoAcids,1993,5(2):217-237.[2]LiP,MaiK,TrushenskiJ,etal.Newdevelopmentsinfishaminoacidnutrition:towardsfunctionalandenvironmentallyorientedaquafeeds[J].AminoAcids,2009,37(1):43-53.[3]AlamMS,TeshimaS,KoshioS,etal.Effectsofsupplementationofcoatedcrystallineaminoacidsongrowthperform-anceandbodycompositionofjuvenilekurumashrimpMarsupenaeusjaponicas.AquacultureNutrition,2004,10(5):309-316.[4]BenitaS.Microencapsulation:Methodsandindustrialapplication[M].MarcelDekker,Inc.,1996:1-20.[5].[D].,2006.[6],,,.[J].,2009,28(4):466-473.ZHUXuan,CAOJun-ming,XUEFeng-meng,etal.Effectoflysine/methionineontilapia(Oreochromisniloticus)growth[J].JournalofFoodScienceandBiotechnology,2009,28(4):466-473.(inChinese)[7]AdemG,GalleR,OdileC,etal.Applicationsofspray-dryinginmicroencapsulationoffoodingredients:Anoverview[J].FoodResearchInternational,2007,40(9):1107-1121.[8],.[J].,2009,24(7):45-49.CAOLong-kui,LiLi.Microencapsulationandstabilityevaluationofmaizeyellowpigment[J].JournaloftheChineseCe-realsandOilsAssociation,2009,24(7):45-49.(inChinese)[9],,.[J].,2006,25(2):60-65.ZHUWe-ihong,XUSh-iying,JIANGBo.Preparationofmicroencapsulatedmintoilusingstar