蒺藜总黄酮和总皂苷提取工艺的优化

Ξ1,1,1ΞΞ,2(1.,130118;21,130118):,,3,,,7613%,2,270%,30,20min50min,:;;;;;;:R28412:A:100025684(2009)0120045206OptimumExtractingTechnologyofTotalSaponinsandTotalFlavonoidsforTribulusterrestrisbyOrthogonalTextYANGLi1,WANGChun2yu1,HANMei1,YANGLi2min2(11KeyLaboratoryforPhytochemistryandEcologyofJilinProvince,JilinAgriculturalUniversity,Changchun130118,China;21KeyLaboratoryforEcologicalRestorationandEcosystemManagementofJilinProvince,JilinAgriculturalUniversity,Changchun130118,China)Abstract:TheoptimalextractiontechnologyofTribulusterrestriswasstudiedusingthecontentoftotalsaponinsandtotalsflavonoidsastheindicators1Respectiveorthogonaltextwascarriedonwithmi2crowave,ultrasonicwaveextractionandsoxhletmethods,andalltheextractioneffectswerecomparedforthefiltrationofextractingtechnology1Theresultindicatedthatmicrowaveextractionhadtheadvantageofhigherefficiency,whichhadthehighestextractioneffectofsaponinsandthehighestextractioneffectofflavinoidsof7613%1Theoptimalconditionswere70%alcohol,rationsofmaterialtosolvent130,50minextractionafter20minplacement1Themethodissimple,efficient,andcanbeusedtocontrolthequalityofTribulusterrestris1Keywords:TribulusterrestrisL;totalsaponins;totalflavonoids;soxhletmethod;ultrasonicwave;mi2crowave;orthogonaltext(TribulusterrestrisL1),,,,,[1][2],ΞΞΞ:(2006BA106A05205):,,,:232222009,31(1):4550@vip1sina1com:2007020:20080428,,,,1111(Dioscin)(Rutin),11211211(1)1014mg,50mL,,0,1,2,3,4,5,6mL,25mL,0104g/mLNaNO21mL,,6min011g/mLAl(NO3)31mL,,6min011g/mLNaOH10mL,,15min1,(,UV21700),510nm,(y),(x),y=15144x-010144,R2=019997,71629451777g/mL(2)912mg,50mL,,0,0120,0140,0160,0180,1100,1120mL6,5mL,,2530min,1,,409nm(y),(x),y=271242x-010503,R2=019996,713644116g/mL112123,[32],3,,,,,9,(1),,013mm,1g,3h,50ml,,,11Table1.FactorsandlevelsofsoxhletmethordLevelA()/%ConcentrationofalcoholBRatioofliquidandsolidC/hRefluxtimeDFrequency15070212701404239521063(2)1g,3h,,,50mL,,22Table2.FactorsandlevelsofultrasonicwaveLevelA()/%Concent2rationofalcoholB/minSupersonictimeCRatioofliquidandsolidD/Supersonictempera2tureE/HzSupersonicfrequency130103020302502050305037040704070495601005090(3)1g,3h,,,5L6420092fUy,Fy40mJournaloJilinAgriculturalniversit2009ebruar,,,33Table3.FactorsandlevelsofmicrowaveLevelA()/%ConcentrationofalcoholB/minTimeofplacementC/minTimeofextractionDRatioofliquidandsolid150101030270203040395305050221145A3B2C3D3,A2B2C3D3(67),2,4,A3B2C3D3,95%14036h4Table4.Resultsoforthogonaltestofflavonoidswithsoxh2letmethordNo1ABCDw()/(mgg-1)Contentofflavonoids111111156121222216743133321921421233112752231219056231231565731324132683213419699332131654K1141312181028201190161239K2191194211096181910211129K3251897201279201303221035R119310151101232019665Table5.ResultsoforthogonaltestofsaponinswithsoxhletmethordNo1ABCDw()/(mgg-1)Contentofsaponins111111112442122211186231333111654421231219825223113119062312111140731321217258321312174993321101914K1104128110185105140106104K2111194113140107127107118K3109116101113112171112116R01851136018101686Table6.VarianceanalysisoftotalflavonoidswithsoxhletmethordSourceSSDFMSFPA1112772516382911170101B0184220142121173C0119920110001515D31241211620813680101Error117439011947Table7.VarianceanalysisoftotalsaponinswithsoxhletmethordSourceSSDFMSFPA3133721166811257B9133024166531514C3119921160011205D2134821117401884Error2318981811328212,74:fUy89JournaloJilinAgriculturalniversitA3B1C3D4E2,A3B4C1D3E2(1011),,A3B1C3D4E2,70%,70,5050Hz10min8Table81ResultsoforthogonaltestofflavonoidswithultrasonicwaveNo1ABCDEw()/(mgg-1)Contentofflavonoids111111212192122222141431333321468414444213275212343132662214331158723412218188243212194893134271363103243161181113312451317123421351253134142331623144231431946154324141216164413241319K1281284491592451042421710461694K2361752471099451625421905501742K3721342441459501175481880431504K4481313441540441848511195441751R31672014280144401707016039Table9.ResultsoforthogonaltestofsaponinswithultrasonicwaveNo1ABCDEw()/(mgg-1)Contentofsaponins111111811002122229110331333381852414444819195212341012296221431013087234129128782432110113193134215116010324311410461133124141052123421314172513414239129914423149160515432411013391644132121009K1104192128136133141125115127185K2119186129119133119127175136167K3173195127159131124135141129155K4123175137135124165134118128142R5151131618420092fUy,Fy70810708074JournaloJilinAgriculturalniversit2009ebruar10Table10.Varianceanalysisoftotalflavonoidswithultra2sonicwaveSourceSSDFMSFPA911492330149734112090101B11491301497515620101C11592301531519360101D415813115271710820101E21503301834913360101Error21860320108911Table11.VarianceanalysisoftotalsaponinswithultrasonicwaveSourceSSDFMSFPA225107437510258715860101B5114131171421001C4119731139911633D6114532104821391E4119631139911633Error27141132018572131213,A1B3C1D1,A2B3C2D3(1415),CAC,,A2B3C2D1,70%3020min50min12Table12.Resultsoforthogonaltestofflavonoidswithmi2crowaveNo1ABCDw()/(mgg-1)Contentofflavonoids11111510912122241848313332188142123412005223121978623125101873132212578