半抗原的设计、修饰及人工抗原的制备

DOI:10.3724/SP.J.1096.2010.012113(,610064)()();;;;;2009210226;2010201203(No.20675054)3E2mail:[e-mail]1,(),(),[1],[2][324][5][6][7](1000),,T,,,,,,2,,TB,[8]()()(),,,,,22.1,,,,,1/3,1/11[9],,COOH[10],NH2[11],OH[4],,;,,,3820108(FENXIHUAXUE)ChineseJournalofAnalyticalChemistry812111218,,,,,,Goodrow[12],:(1),;(2),;(3),;(4)2.22.2.1,,,,[13],(COOH,NH2,OH,),,,Zhang[14],5,5,(X)(Y)(1a),,,,32Cl[15,16][17]NO2NH2,(1b),3,Cl,,,,,,,,,Kusharyoto[18](Atrazine),ClC,,C,,Cl,(1c)Newsome[19]3,S1S3,(1d)1(a)(b)(c)(d)Fig.1Chemicalmodificationoffenthion(a),imidacloprid(b),trazine(c)andmonuron(d),,,,,;,,2121382.2.2,,(1),()(NO2NH2)(ClHS(CH2)nCOOH)(CNCOOH);(2),,,[20]Jung[21],,,,,Miyamoto[22],;(3),[23],5Gui[24],3,O2(TZM1),O22O2(1221,2,42232)(TZM2),(2a);,I22(2b)[7],,,,,2(a)1(b)Fig.2Synthesisofhaptentriazophos(a)andhaptenSudanI(b),(UV,IR,NMR,MS)2.2.3(),:(1),,,(),;(2),,;,()()Frieia[25]36Johnson[26]4;[27]1,,,Kim[13],,,,31218:;(3)(),,33.12,,,,,,[28],(Globulinfractions)(Bovineserumalbumin,BSA)(Ovalburmn,OVA)(Keyholelimpethemo2cyanin,KLH)(Rabbitserumalbumin,RSA)(Humanserumalbumin,HSA)(Thyroglobulin)(Fibrinogen),(BSA)BSA,,,pH,(N,N2)(KLH),KLH,(PLL),3.2(Chemicalconjunction)(Chemical2biologicalmethods)(Immunologicallabelingmethods),()()3.2.1,,;,();,;,SAMSA(S2);,O2()2,1[29]1Table1Commonmethodsofhapten2derivativecouplingwithcarrierproteinCouplingmethodsActivegroupsReactionprincipleAppliedexamplesCarbodiimidemethodCOOH[30]ActiveestermethodCOOH[24,31]MixedanhydridemethodCOOH[3234]Azo2benzoicacidmethodCOOH[7]4121381(ContinuedtoTable1)CouplingmethodsActivegroupsReactionprincipleAppliedexamplesDiazotizationmethodNH2[5]Glutaraldeh2ydemethodNH2[35,36]CyanogenchloridemethodOH[37]N,N2N,N2Carbonyldii2midazolemethodOH[4,38]AzidemethodCOOCH3[39]O2O2Carboxy2methylhydroxylam2inemethodCO[40,41]242Hydrazin2obenzoicacidmethodCO[42,43]3.2.2:(1);(2)pH(pH=6.08.0);(3),;(),,,();(4);,3.2.3,Wittmann[44]351,Wust[45]700(Atriazine),Schneider[46]1020,,,,,[47],,1,,,4,(2d),,,,51218:,;,,[7][48]SDS2PAGE[49]ESI2MS[50]MALDI2TOF2MS[5153],,;NH2,;;5,,,:(1),,;(2),,,3D[54,55],(),,,,,,,[56,57],[5860][61,62],,[63],,,References1CHENFu2Sheng(),GAOZhi2Xian(),WANGJian2Hua().FoodSafetyMonitoringandMordenBiotechnology().Beijing():ChemicalIndustryPress(),2004:102ShuTW,WenJG,YiRG,GuoNZ.Anal.Chim.Acta,2007,587(1):2872923XingWW,HeL,YangH,SunCJ,LiDW,YangXM,LiY,DengAP.J.Sci.FoodAgri.,2009,89(13):216521734ZhaoD,HeL,PuC,DengAP.Anal.Bioanaly.Chem.,2008,391(7):265326615PuC,WuYF,YangH,DengAP.Anal.Chim.Acta,2008,628(1):73796ShimWB,KimJS,KimJY,ChoiJG,JeJH,KuzminaNS,EreminSA,ChungDH.FoodSci.Biotechnol.2008,17(3):6236307HanD,YuM,KnoppD,NiessnerR,WuM,DengAP.J.Agric.FoodChem.,2007,55(16):642464308JIAOKui(),ZHANGShu2Sheng().TheTechnologyandApplicationofEnzyme2LinkedImmunoassay().Beijing():ChemicalIndustryPress(),2004:29SzurdokiF,BekheitHKM,MarcoMP.J.Agric.FoodChem.,1992,40(8):1459146510JiL.FoodandAgriculturalImmunology,2009,20(1):354711WellerMG,DiemerM,WerschingC,NiessnerR,SochorH.J.AgricFoodChem.,2003,51(23):6668667512GoodrowMH,HammockBD.Anal.Chim.Acta,1998,376(1):839113KimYJ,ChoYA,LeeHS.Anal.Chim.Acta,2003,494(122):294014ZhangQ,LiuFQ.Anal.Chim.Acta,2007,596(2):30331115LiK,QingXL.J.Agric.FoodChem.,2000,48(8):3378338261213816ZHUGuo2Nian(),GUIWen2Jun().ChinaAgriculturalScience(),2005,38(3):51151517LeeJK,AhnKC,ParkOS,KangSY,HammockBD.J.Agric.FoodChem.,2001,49(5):2159216718KusharyotoW,PleissJ,BachmannTT,SchmidRD.ProteinEngineering,2002,15(3):23324119NewsomeWH,CollinsPG.FoodAgric.Immunol.,1990,2:7520ZHANGChun2Zheng(),LIUXian2Jin(),YUXiang2Yang().J.NanjingAgriculturalUniversity(),2002,25(4):374021JungF,MeyerH,HammR.J.AgricFoodChem.,1989,37(4):1183118722MiyamotoT,KuwaharaT,YamamotoI.PesticideBiochemistryandPhysiology,2001,69:17418223YUWan2Jun().ChineseJournalofHealthInspection(),1995,5:13113424GuiWJ,JinRY,ChenZL,ChengJL,ZhuGN.Anal.Biochem.,2006,357:91425FrieiaJ.PesticSci.,1989,26:30330726JohnsonJC,VanEmonJM,PullmanDR,KeeperKR.J.Agric.FoodChem.,1998,46(8):3116312327XUEXiao2Ping(),ZHANGMei2Shun().ChineseJournalofBiologicalEngineering(),2003,23(6):687528HarrisonRO,GoodrowMH,HammockBD.J.Agric.FoodChem,1991,39(1):12212829HONGXiao2Zhuang(),SUNMan2Wen().ProteinlinkingTechnology().ChinaMedicalScienceandTechnologyPress(),1993:21030LiuY,LouY,XuD,XuD,QianGL,ZhangQ,WuRR,HuBS,LiuFQ.MicrochemicalJournal,2009,93(1):364231ChengCC,HsiehKH,LeiYC,TaiYT,ChangTH,SheuSY,LiWR,KuoTF.J.Agric.FoodChem.,2009,57(13):5687569232XIEZhong2Liang(),LIWen(),HEJi2Guo().FoodScience(),2009,30(1):22823133HEXiao2Wei(),LIUXiao2Yun().SouthChinaUniversityofTechnology(),2007,35(11):12512834YANGTing2Ting(),SUNXiu2Lan(),SHAOJing2Dong(),WANGHong2Xin().J.CellularandMolecularImmunology(),2008,24(9):92492735SrtasserA,UsleberE.FoodandAgriculturalImmunology,2003,152:13514336WUYang(),YUANJun2Lin().EnvironmentalScienceandTechnology(),2007,30(4):404237ZHANGJin2Hui(),JIANGZhong2Hua().BiomolecularImmobilizationTechnologyandApplications().Beijing():ChemicalIndustryPress(),1998:576038WUYuan2Yuan(),BAOYong2Ming().ChemicalBulletin(),2005,6:45846339JohnsonJR.Br.J.Ca