16S-rDNA引物

一般细菌鉴定选择通用引物，最常用的通用引物为27F/1492R。27F：5'-AGAGTTTGATCCTGGCTCAG-3'1492R：5'-GGTTACCTTGTTACGACTT-3'Lane,D.J.(1991).16S/23SrRNAsequencing.Nucleicacidtechniquesinbacterialsystematics.E.StackebrandtandM.Goodfellow,eds.NewYork,NY,JohnWileyandSons:115-175.27f5'AGAGTTTGATCMTGGCTCAG342r5'CTGCTGCSYCCCGTAG357f5'CTCCTACGGGAGGCAGCAG519r5'GWATTACCGCGGCKGCTG530f5'GTGCCAGCMGCCGCGG1100r5'GGGTTGCGCTCGTTG1114f5'GCAACGAGCGCAACCC1392r5'ACGGGCGGTGTGTRC1406f5'TGYACACACCTCCCGT1492r5'TACGGYTACCTTGTTACGACTT1525r5'AAGGAGGTGWTCCARCCUniversalprimersfromLeser02EditedfromLane91,namingfromAlm96,PMID8837410S-*-Univ-1492-a-A-19GGTTACCTTGTTACGACTT-3S-D-Bact-0008-a-S-20AGAGTTTGATCMTGGCTCAG-3S-D-Bact-0907-a-S-20AAACTCAAAGGAATTGACGG-3S-*-Univ-0519-a-A-18GTATTACCGCGGCTGCTG-3S-D-Bact-0786-a-A-20GACTACCAGGGTATCTAATC-3S-D-Bact-1054-a-A-20ACGAGCTGACGACAGCCATG-3MycoplasmauniversalprimersJohanssonK-E,CharacterizationofmycoplasmasbyPCRandsequenceanalysiswithuniversal16SrDNAprimers,Chapter18,MycoplasmaProtocols,1998.U1F12-27ttgatcctggctcaggaydaacgU2F327-348ccaractcctacggraggcU3R512-527attaccgcggckgctgU4F792-810gtagtccacgccgtaaacgU5R902-924cttgtgcgggyycccgtcaaU6F1154-1172gaggaaggygrggaygaygU7R1359-1374acaagrcccgagaacgU8R1502-1524gaaaggaggtrwtccayccscac