HPV感染与宫颈癌的关系及诊治进展

3,,,,:;,,,:HPV(510515)HPV,,HPV,HPVHPV1HPV111,,[1]HPVE6E6,-[2],p53,,,HPVE7p27,p15p21CKI,E7,E2F[3],E7E6,,[4],112,HPV[5],HPVp53,,(CIN)HPVCIN[6]p53(SiHa),SiHahTERTmENA,p53SPI,hTERT,[7],,HPVp53Rb113HPV,,,HPVHPV,HPV:E71737p48,-IFN,(IFN)E6TyK2,JAD-STAT,IFNE6IRF-3,IFN-/E6IRF-3,IFN-E6E7TGF-2[8],TGF-2HPV,HPVSouthernBoltHPV,,,HPVHPV-DNASouthernBolt,,HPV-DNA,,,Player[9]DNAHPV-DNA,,HPV(PCR),HPV,HPV,,Jorden[10]HPV,,HPV;Tucker[11]PCR5HPV,HPV6,11,16,184,,3,,,,,570%,,HPV,CIN,742200432533115-FU,,,,,HPV312,,,CIN97%,,3CIN,,34mm,90%95%313,,CIN3314HPV,HPV,HPVLui[12]12(IL-12),IL-12,HPVDNADiaz[13]5%733421,315HPVHPVDNAHPVHPV20021122,HPV-16,HPV-16[14],HPV-16HPV,HPV,HPVHPV-16,1NurseP.Alongtwentiethcenturyofthecellandbeyond.Cell,2000,100(1):712KaoWH,BeaudenonSL,TalisAL,etal.Humanpapillomavirustype16E6inducesself-ubiquitinationoftheE6APubiqutin-proteinligase.JVi2rol,2000,74(14):64083CrishJF,BoneF,BalasubramanianS,etal.Suprabasalexpressionofthehumanpapillomavirustype16oncoproteinsinmouseepidermisaltersex2pressionofcellcycleregulatoryproteins.Carcinogenesis,2002,21(5):10314DuensingS,DuensingA,CrumCP,etal,Humanpapillomavirustype16E7oncoprotein-inducedabnormalcentrosomesynthesisisanearlyeventintheevolvingmalignantphenotype.CancerRes,2001,61(6):23565OhST,KyoS,LaiminsLA.Telomeraseactivationbyhumanpapillo2mavirustype16E6protein:inductionhumantelomerasereversetranscrip2taseexpressionthroughMycandGC-richSPIbindingsites.JVirol,2001,75(12):55596FerenczyA,FravcoE.Persistenthumanpapillomavirusinfectionandcer2vicalneoplasia.LancetOncol,2002,3(1):117KanayaT,KyoS,HamadaK,etal.Adenoviralexpressionofp53repressestelomeraseactivitythroughdown-regulationofhumantelomerasereversetranscriptasetranscription.ClinCancerRes,2000,6:12398NeesM,GeogheganJM,MunsonP,etal.Humanpapillomavirustype16andE7proteinsinhibitdifferentiation-dependentexpressionoftansform2inggrowthfactor-beta2incervicalkeratinocytes.CancerRes,2000,60(15):42899PlayerAN,ShenLP,KennyD,etal.Single-copygenedetectionusingbranchedDNA(bDNA)insituhybridization.JHistochemCytochem,2001,49(5):60310JordensJZ,LanhamS,PickettMA,etal.Amplificationwithmolecularbeaconprimersandreverselineblottingforthedetectiontypingofhumanpapillomaviruses.JVirolMethods,2000,89(12):2911TuckerRA,UngerER,HollowayBP,etal.Real-timePCR-basedfluo2rescentassayforquantitationofhumanpapillomavirusestypes6,11,16and18.MolDiagn,2001,6(1):3912LuiVW,HeY,FaloL,etal.SystemicadministrationofnakedDNAen2codinginterleukin12forthetreatmentofhumanpapillomavirusDNAposi2tivetumor.HumGeneTher,2002,13(2):17713DiazWrrastiaC,Aranyl,RobazettiSC.Clinicalandmolecularresponsesinhigh-gradeintraepithelialneoplasiatreatedwithtoplicalimiquimod5%.ClinCancerRes,2001,7(10):303114KoutskyLA,AultKA,WheelerCM,etal.Acontrolledtrialofahumanpapillomavirustype16vaccine.NEnglJMed,2002,347:1645(:2003-04-28:)PCRHPV13HPVCobasAmplicorHPVPCR()HPV,13HPVHPV,VonProndzynski:HPV842GuangdongMedicalJournalMar.2004,Vol.25,No.3