论文格式模板(例文)

贵阳护理职业学院2014届高职高专学生毕业论文Noggin、BMP2和IGF-I对成骨细胞生长的调节作用系别：专业：指导老师：学生：中国﹒贵州﹒贵阳2013年6月论文编号：2014届贵阳护理职业学院学生毕业论文II目录中文摘要.........................................................................................................................................III英文摘要.........................................................................................................................................IV前言.........................................................................................................................................61.BMP蛋白.............................................................................................................................61.1BMP结构和功能.......................................................................................................61.2BMP诱导成骨机制...................................................................................................72.Noggin基因的概况..............................................................................................................82.1Noggin基因的结构....................................................................................................82.1.1Noggin基因结构特征.............................................................错误!未定义书签。结果.....................................................................................................................................9致谢...........................................................................................................错误!未定义书签。参考文献.....................................................................................................错误!未定义书签。2014届贵阳护理职业学院学生毕业论文III中文摘要目的：研究noggin、骨形态发生蛋白2（bonemorphogeneticprotein2，BMP2）、胰岛素样生长因子I（insulinlikegrowthfactorI，IGF-I）对成骨细胞增殖和分化的调节作用。方法：1、将人成骨肉瘤细胞MG63于RPMI1640培养基中培养，添加不同浓度的noggin、BMP2、IGF-I蛋白进行药物干预，用四甲基偶氮唑盐（methylthiazolyl-tetrazolium，MTT）法研究细胞的增殖率。2、选用最适浓度的noggin、BMP2、IGF-I蛋白作用成骨细胞，运用ELISA方法和RT-PCR技术分别检测成骨细胞分化标志物骨碱性磷酸酶（bonealkalinephosphatase,BALP）和骨钙蛋白（osteocalcin,OC）的蛋白含量和mRNA的表达情况。结果：1、各药物干预组OD值与对照组相比有统计学差异（P＜0.05），对成骨细胞增殖的作用效应呈剂量和时间依赖性，其作用的最佳时间均在72h。各药物组内之间比较，确定了最佳作用浓度，BMP2为2×10-9mol/L，对MG63细胞的增殖率达179.59℅；noggin为8×10-9mol/L，对其增殖的抑制率达到15.51℅；IGF-I为1×10-8mol/L，其增殖率达189.80℅。结论：BMP2、IGF-I对MG63细胞增殖有促进作用，而noggin则抑制MG63细胞的增殖。Noggin、BMP2和IGF-I促进BALP的分泌。Noggin对OC蛋白的分泌有抑制作用，而BMP2、IGF-I则促进OC蛋白的分泌。关键词：Noggin蛋白，BMP2蛋白，IGF-I蛋白，成骨细胞，增值，分化2014届贵阳护理职业学院学生毕业论文IV英文摘要Object:Theaimsistostudytheeffectsofnoggin,bonemorphogeneticprotein2(BMP2)andinsulin-likegrowthfactorI(IGF-I)ontheproliferationratesanddifferentiationofosteoblasts.Methods:ThehumanosteosarcomacelllineMG63werechosenusingtheRPMI1640mediumwith10℅fetalbovineserum(FBS).Then,theculturedMG63cellswereexposedtoBMP2,nogginandIGF-Iatdifferentconcentrationswithzeroconcentrationascontrolgroup(CON).AbilityoftheproliferationofMG63cellswasdetectedbymethylthiazolyltetrazoliummethod(MTT).theconcentrationofBALPandOCofosteoblastsweredeterminedbyELISAmethod.atdifferenttimepoints(12h,24h,48h,72h)afteroptimaldosageofnogginBMP2,IGF-Iwereaddedintotheculturemedium.AndtheexpressionlevelofBALPandOCwereinvestigatedbyreversetranscriptionPCRatthesametime.Results:1.TheproliferationabilityofMG63cellstreatedwithBMP2,nogginandIGF-IgroupsweresignificantlydifferentcomparedwiththatofCONgroup(P＜0.05).TheeffectsofthreefactorsonMG63cellwasdose-dependentlyandtime-dependentlyafterculturedfor72h.ThemosteffectivegrowthofMG63cellsshowedat2×10-9mol/LofBMP2withtheproliferationrateof179.59℅and1×10-8mol/LofIGF-1withtherateof189.80℅,while8×10-9mol/Lnogginwiththeinhibitoryproliferationrateof15.51℅,respectively.2.TheconcentrationofBALPtreatedwithBMP2,nogginandIGF-IgroupsweresignificantlydifferentcomparedwiththatofCONgroup(P＜0.05)for48h,especially,thenoggingroupwasextremelysignificant(P＜0.01),andtheBALPmRNAexpressionwerealsosignificantlydifferent(P＜0.05).3.TheconcentrationofOCtreatedwithnoggingroupsweresignificantlydifferentcomparedwiththatofCONgroup(P＜0.01)for24h,buttheOCmRNAexpressionwerenotsignificantlydifferent(P＞0.05).TheconcentrationofOCofBMP2groupweresignificantlydifferentcomparedwiththatofCONgroup2014届贵阳护理职业学院学生毕业论文VsignificantlydifferentcomparedwiththatofCONgroup(P＜0.01),andtheOCmRNAexpressionwerealsosignificantlydifferent(P＜0.05).TheconcentrationofOCofIGF-IgroupweresignificantlydifferentcomparedwiththatofCONgroupsignificantlydifferentcomparedwiththatofCONgroup(P＜0.01),andtheOCmRNAexpressionwerealsosignificantlydifferent(P＜0.05).Conclusion:BMP2andIGF-IpromotedtheproliferationofMG63cellswhereasnoggininhibited.Noggin,BMP2,andIGF-IenhancedtheconcentrationofBALPinosteoblasts.BMP2andIGF-IimprovedtheconcentrationofOCinosteoblasts,whereasnoggininhibited.Keywords:Noggin;bonemorphogeneticprotein2(BMP2);insulin-likegrowthfactorI(IGF-I);MG63cell;proliferation;differentiation.前言生长是动物个体发育特有的生理特点，其主要表现在体高体长的增长方面。而体高体长的增长则源于骨骼的纵向生长，从而形成相应的骨骼系统，达到体高体长的增长的目的。骨骼系统也是机体非常重要的一个组成部分，它支撑躯体，保护内脏，并且和骨骼肌一起协调配合完成其运动功能。在骨骼的生长发育过程中，骨组织不断进行着新骨形成和旧骨吸收的骨重建过程，即骨形成（boneformation）与骨吸收（boneresorption）的动态平衡代谢过程。骨形成由成骨细胞介导来完成。在骨形成过程中，成骨细胞主要经历细胞增殖、细胞外基质成熟、细胞外基质矿化和细胞凋亡四个阶段。在幼年生长时期