DPPH和FRAP法测定41种中草药抗氧化活性-陈玉霞

30620116RESEARCHANDEXPLORATIONINLABORATORYVol．30No．6Jun．2011DPPHFRAP4145000241。70%DPPHFRAP。41DPPHFRAP。、、、、、10mg/mLDPPH70%FRAP＞200。FRAPDPPHR932R917A1006－7167201106－0011－04DeterminationofAntioxidativeActivityof41KindsofChineseHerbalMedicinesbyUsingDPPHandFRAPMethodsCHENYu-xiaLIUJian-huaLINFengDUXiang-dangCollegeofAnimalHusbandryandVeterinaryHenanAgriculturalUniversityZhengzhou450002ChinaAbstractTheantioxidativeactivitiesof41kindsofChineseherbalmedicinesweredeterminedandselected．TheantioxidativesubstanceswereextractedfromChineseherbalmedicinesby70percentethanolandomniruptor．ThespectrophotometricmethodwasusedtostudytheantioxidantactivitiesofmedicinalherbsbyDPPHandFRAPmethods．TheresultsshowthatthesekindsofmedicinalherbsincludingPolygonummultiflorumRheumpamatumEpimediumgrandiforumCrataegustifigaPaeonialactifloraCornusofficinalishavestrongantioxidantactivities．Whentheconcentrationis10mg/mLtheextractsfromthesesixherbsreduceatleast70%ofDPPHandtheFRAPvalueofantioxidativecapacityismorethan200andtheothershaveweakerantioxidativeactivity．KeywordsspectrophotometryChineseherbalmedicinesDPPHmethodFRAPmethodantioxidativeactivity2010－12－26“”2006BAK02A03-31973－。[e-mail]0。BHT、BHA、1-2。3-4。5-8。41DPPHFRAP。11．1JY92-ⅡUV-2800EppendorfFZ10230。1．211-pheny-2-picrylhydrazylDPPHSigmaFRAP300mmol/L10mmol/LTPTZ20mmol/LFeCL3。TPTZ246-tripyridyl-s-trizineSigma411。141DPPHFRAPDPPH/%FRAPDPPH/%FRAP94．20±1．25700．00±37．3715．11±3．7543．00±3．2392．14±0．73660．70±16．1610．77±2．2940．42±2．4287．48±3．90209．14±5．259．31±0．2227．28±9．0975．16±4．75332．00±3．239．13±3．6738．35±1．5174．23±5．12242．70±26．469．13±1．0936．28±5．8572．11±5．17311．10±17．778．47±4．3126．07±0．5068．26±7．21284．10±36．567．96±3．7925．07±0．5057．26±6．54143．64±3．137．85±1．5542．92±1．3147．09±7．83116．28±6．067．64±1．6416．21±3．7346．00±3．88101．20±15．457．55±1．7541．21±1．7142．65±5．76143．78±0．107．04±3．0723．85±3．4337．41±6．70106．50±5．956．89±1．6549．50±2．9234．15±5．89194．00±11．816．27±3．8816．64±0．9028．83±1．7998．50±2．526．56±0．055．71±0．6027．40±2．41109．14±3．436．07±3．4718．35±5．5525．53±3．3474．35±5．955．71±0．6012．36±5．3522．15±0．0287．57±2．225．61±2．7523．07±0．3017．60±3．1951．21±0．905．20±0．109．14±6．6616．72±3．0227．42±3．634．69±0．419．14±1．4115．88±3．3549．64±1．512．75±0．985．78±1．7115．40±2．2654．64±4．941．31。6060℃24h。2。10．5g50mL70%20、15、15mL33min10s400Hz10s18370%50mL。31。①FRAP。100μLFRAP3mL20min593nm。FeSO41Y=0．007X－0．019R2=0．994FRAP1FRAP=1mmol/LFeSO4FeSO4mmol/L。②DPPH。100μL2mL0．2mmol/LDPPH20min517nm2mLDPPH+100μL。1FeSO4%=1－A517/A517×100%A517DPPHA517DPPH。216DPPHFRAP4122．11。2．2141DPPH、、、、、10mg/mLDPPH70%FRAP200、、、、、、DPPH30%FRAP100。。1DPPHFRAP。2．31。DPPH。2DPPH517nm。DPPHDPPH。。FRAPpHFe3+TPTZFe3+-TPTZ593nm。3。30、、、、、、、5DPPH228。。BenzieFRAP、、FRAP9。DPPH10-11FRAP12。4、、。。、、、、、13-15。3241。、、、、、6DPPH70%FRAP＞20014．6%。。References1．20J．200221250-52．2．DPPHJ．2010311132-135．3．DPPH26J．2010363148-150．4．DPPHJ．200536135-38．5．30J．200013170-73．6．J．2006456131-134．7．DPPHJ．2000276658-661．8．22J．200622148-52．9．3130J．2004162135-136．10YenWJChangLWLeeCPetal．InhibitionoflipidperoxidationandnonlipidoxidativedamagebycarnosineJ．JAOCS2002794329-333．11．6J．2010193204-206．12BenzieIFStrainJJ．TheferricabilityofplasmaasameasureofantioxidantpowertheFRAPmethodJ．AnalBiochem199623970-76．13．J．2010392164-167．14．J．200618514-518．15．B—FetonJ．20101檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿檿122-124．1073/℃/℃40～581430～603033～39632～3534。10～50℃。2mm4°。。References1MehrdadEhsani．ModrenelectrichybridelecticandfuelcellvehiclesM．BocaRatonCRCPress2005．2．M．2009．3．D．2009．4．J．20091028-30．5．LiFePO4/CJ．2008295-98．6PesaranAABurchS．AnapproachfordesigningthermalmanagementsystemsforellecticandhybridvehiclebatterypacksC∥OSTI．19991-16．7．M．2002．8．RAV-4J．2008713-16．9．J．20093224-227．10．J．20053119-123．11HaipingXuLiKongXuhuiWen．FuelcellpowersystemandhighpowerDC/DCconverterJ．IEEETransitionsonPowerElectronic200419951-53．41