基因工程基因工程

523416789DNADNADNADNADNADNADNADNADD88CCBBAAEEAA88BB885--1-PRPP5--1-PRPPHMPHMPHRHRGMPGMPAMPAMPUMPUMPTMPTMPTRTRHPRTHPRTTKTKAPAPAPAPTKtk-TKtk-HATtkHATtkHPRTHPRTHAThprtHAThprthprt-hprt-APH-G418APHG418APH-G418APHG418DHFRDHFRDHFRDHFRHPH-BBHPHBHPH-BBHPHBTK-TKTMPTK-TKTMPXGPRT-XGPRTGMPXGPRT-XGPRTGMPADA-ADAXyl-AADA-ADAXyl-ACHOCHOFDAGRASFDAGRASCC88DNADNADNASV40DNASV40DNABKVDNABKVDNADNADNADNADNA93DNA936C2Ad256C2Ad25Ad5Ad5DNADNADNAITRITRITRITRE1E1E2E2E3E3E4E4IVa2IVa2VAVAL1L1L2L2L3L3L4L4L5L5DNA36kb105%DNADNA36kb105%DNAITRE1-E4ITRE1-E4E3L1-L5E3L1-L5IVa2VARNAE3IVa2VARNAE32.2kb2.2kb4kb4kbDNADNADNADNADNADNADNADNASV40DNASV40SV40VP1SV40VP1VP2VP35224bpDNAVP2VP35224bpDNASV40SV40H2H3H4SV40H2H3H4DNADNASV40SV40DNASV40DNASV40SV40DNAt/Tt/TSV40DNASV40DNAorioriVP2VP2TTVP3VP3VP1VP1ttSV40SV40105105DNADNADNASV40DNASV40SV40DNA-DNASV40DNASV40DNADNADNASV40SV40SV40SV40AprAprorioriviralgeneviralgenegptgptSV40oriSV40oripV2-GPTpV2-GPTpBR322pBR322pBR322pBR322SV40SV40pV2-GPTSV40DNADNApV2-GPTSV40DNADNAgpt-gpt-β-.β-.DNASV40DNASV40SV40DNASV40SV40orioriSV40SV40DNADNADNASV40DNASV40SV40DNADNABKVDNABKVBKVBKVDNADNA500500DNABKVDNABKVBKV-E.coliBKV-E.coliBKVoriBKVoriBKVgeneBKVgeneDREDREMMTPMMTPAprAprE.colioriE.colioriSV40PSV40PNeorNeorP450dioxinP450dioxinDREMMTPDREMMTPSV40NeorG418SV40NeorG418β1-Bβ1-BDNADNADNA185kbDNA185kbDNADNADNADNAT7RNAT7RNADNAT7RNAT7T7RNAT7DNAT7RNADNAT7RNADNADNAT7RNAT7RNAT7T7DD88DNADNADNACaCl2DNACaCl2DNADNADNADNA374-16374-16DNA7DNA7DNADNADNADNADNADNADNADNADNADNADNADNADNADNADNADNAHeLaHeLaDNADNADNADNADNADNADNADNADNADNAEE88VIIIVIIIMTXMTXdhfrdhfrDHFRDHFRdhfrdhfrDNAdhfrdhfrDNADHFR-MTXDHFR-MTXdhfrdhfrdhfr-dhfr-0.05µMMTX0.05µMMTX5µMMTX5µMMTX0.25µMMTX0.25µMMTX100100GS-MSXGS-MSXGSMSXGSMSXGSGSGSMSXGSMSXGS-MSXDHFR-GS-MSXDHFR-GSMSXGSMSXMTXMTXAprAprM13oriM13oriColE1oriColE1oriCMVPCMVPPolylinkerPolylinkerGeneGeneIntronIntronSV40TSV40TPolyAsignalPolyAsignalmRNAmRNAAAAAAAAAAAAAAAAAAAmRNAmRNACMVCMVSV40polyASV40polyAmRNAmRNAmRNAmRNA300300βγβγVIIIIXVIIIIXEPOhGHEPOhGH2IL-22IL-2CD4CD4tPAtPANeorNeorββcDNAcDNAββpLpLdhfrdhfrSV40SV40ββcDNAcDNAββpHpHcampathcampathcampathcampathDHFR-MDHFR-MTXTXdhfrdhfrtPAcDNAtPAcDNAtPAtPADHFR-MTXDHFR-MTXCHOCHOtPAtPAtPAtPAVIIIVIIIVIIIVIIIVIIIVIII10%10%VIIIcDNAVIIIcDNAtPAtPAVIIIVIIIVIIIVIII