Ultimate液相色谱柱技术培训资料

WelchMaterials,Inc.UltimateTMColumnforUltimatePerformance2••••••ISO90013••45ØØ6•1970HPLC10µm•70•80HPLC510µm•905µmBB•903µm3.5µm•212µm•HPLCB7HPLC•HPLC––••–––81.2.3.4.5.6.pHpH287.91.2.pHpH1133.4.pH5.6.7.10:•HPLC•(5µm,3µm,2µm)•••:•HPLC•5µm•••11••A,A•(B)•12M+MSiOHAC18BC1813DNA1,000nm300–1,000nm(MW4,000to130,000),60–150nmHPLC60nm147–10µm10–20µmhighthroughput,2µm3–3.5µm5µm15HPLC5-2010-252.0–5.05-2010-250.8–1.03-815,250.1,0.213-103-250.3–0.46(µm)(cm)D(cm)16•:–:70%;ØC18,65%;C8,15%;Cyano,5%,5%,–:20%;Ø-NH2-CN–(,):10%••C18C817?•••••pH18•C18•C1850%•••191N2Tf34)5pH620N17,000-20,00015312,000-14,0001039,000-11,0007.536,000-7,0005317,000-20,00025510,000-12,0001557,000-9,0001058,000-10,00025106,000-7,0001510N(cm)(µm)21TfAs=1.0-1.05As=1.2As=2As=42.02.51.82.21.61.91.41.61.21.31.01.0Tf(at5%)As(at10%)(As)=BAA(Tf)=A+B2A10%5%221.2.303.4.P=3000Lt0dp2P(psi)L(cm),(cP)t0,dp(µm)23?1.——2.——3.——4.——5.——241.B——2.50Å3.3µm10µm——4.——5.——6.7.——8.——pH,25•:–:XB-C18,AQ-C18,XB-C8–:XB-Phenyl,XB-CN•:–5µm–LC/MS3µm–10µm•:–:4.6x250mm,4.6x150mm,4.6x100mm–:4.6x75mm,4.6x50mmUltimateTM26UltimateTM••ØUltimateTMXBC18ØUltimateTMAQC18ØUltimateTMXBC8ØUltimateTMXBPhenylØUltimateTMXBCN27UltimateTMl99.999%lllll3µm10µm28UltimateTM00.050.10.150.20.250.30.350.4101001000PoreSize(Å)PoreVolume(cm3/g)l120Åll50Ål29min02468101214mAU020406080100120140160min02468101214mAU020406080100120140160NNNNOHHO312:UltimateTMXB-C18,4.6x150mm,5µm:45%/55%20mM,pH7.6:1ml/min:215nm:250C:1µl:1)4,4’-2)2,2’-3)1,2-123pH2.7pH7.630:4.6x150mm,5µm:45%/55%20mM,pH7.6:1ml/min:215nm:250C:1µl:1)4,4’-2)2,2’-3)1,2-min02.557.51012.51517.5mAU020406080min02.557.51012.51517.5mAU020406080min02.557.51012.51517.5mAU0204060min02.557.51012.51517.5mAU0204060312312312312WelchUltimateTMXB-C18ThermoHypersilC18EliteSinoChromA-C18AnalyticalTechnologyChromC18PeakdisappearedPeaktailedPeaktailed31UltimateTMXBllllpHpH1.510.0lll32UltimateTMAQ-C18lllllC18,C18l33UltimateTM1.510.01.59.01.510.01.510.01.510.0pH12%7%13%12%17%320m2/g320m2/g320m2/g320m2/g320m2/g120Å120Å120Å120Å120Å3,5,10µm3,5,10µm3,5,10µm3,5,10µm3,5,10µmAQ-C18XB-CNXB-PhenylXB-C8XB-C18341649,8989,780WelchUltimateTMXB-C181839,2209,110PhenomenexLunaC18(2)183148195181(bar)5,9106,3809,4308,650N(Butylbenzene)N(Amybenzene)(4.6x100mm,5µm)6,360WatersXTerraMSC186,720VarianInertsilODS-39,280SupelcoDiscoveryHS-C188,330AgilentXDBC18UltimateTM35UltimateTM:4.6x150mm,5µm:78%20mM,pH2.7/22%:1ml/min:215nm:400C:1.2.3.3-4.2,3-5.6.4-7.min02.557.51012.51517.5mAU050100150min02.557.51012.51517.5mAU050100150min02.557.51012.51517.5mAU0255075100125min02.557.51012.51517.5mAU0501001502002134567213456752134676251347UltimateTMXB-C18UltimateTMXB-C8UltimateTMXB-PhenylUltimateTMXB-CN36UltimateTMXB-C18lllC18lpHpH1.510.0lll37UltimateTMXB-C18llllllllpH=1.3pH=10.0l38:UltimateTMXB-C18,4.6x150mm,5µm:80%/20%:1ml/min:254nm:250C:1µl:1)2)3)4)5)min024681012141618mAU010203040501423539min02468101214mAU0100200300400500600700min02468101214mAU020406080100120140413241321n:UltimateTMXB-C18,4.6x100mm,5µm:30%/70%:1ml/min:215nm:25:1µl:1)2)3)4)40:UltimateTMXB-C18,4.6x100mm,5µm:30%/70%20mM:1ml/min:215nm:25:1µl:1)2)3)min0123456789mAU020406080min0123456789mAU050100150200250300132132pH2.7pH7.641UltimateTMXB-C18:4.6x100mm,5µm:70%20mM,pH7.630%:1ml/min:215nm:250C:1µl:1)2)3)min02468101214mAU010203040min02468101214mAU010203040min02468101214mAU010203040min02468101214mAU010203040WatersXTerraMS-C18Tf=1.95Tf=6.79Tf=1.53132132132WelchUltimateTMXB-C18132Tf=3.06SupelcoDiscoveryHS-C18VarianInertsilODS-342EngelhardtTestMixmin0510152025mAU0102030405060708021345678:UltimateTMXB-C18,4.6x150mm,5µm:55%/45%:1.5ml/min:254nm::1)2)3)4)5)N,N-6)7)8)43min024681012141618mAU020406080100120140:UltimateTMXB-C18,4.6x150mm,5µm:20%20mM,pH7.0/80%:1ml/min:215nm:250C:1)2)3)4)5)54132pH7.044min02.557.51012.51517.5mAU020406080100120min02.557.51012.51517.5mAU0102030405060min02.557.51012.51517.5mAU020406080AnalyticalTechnologyChromC18EliteSinoChromA-C18N=6854,Tf=1.78N=5312,Tf=1.98N=10840,Tf=1.18WelchUltimateTMXB-C18413254132541325:4.6x150mm,5µm:80%MeOH/20%20mMp,pH7.0:1ml/min:215nm:250C:1)2)3)4)5)UltimateTMXB-C1845UltimateTMXB-C181:4.6x100mm,5µm:80%/20%20mM,pH7.0:1ml/min:215nm:250C:1)2)3)4)5)min02468101214mAU0255075100125min02468101214mAU0255075100125min02468101214mAU0255075100125min02468101214mAU0255075100125WatersXTerraMS-C18AgilentRx80XDB-C18N=6272,Tf=1.20N=3891,Tf=1.25N=7256,Tf=1.05413254132541325WelchUltimateTMXB-C1841325PhenomenexLunaC18(2)N=6258,Tf=1.2746min02468101214mAU020406080100120140min02468101214mAU020406080100120140min02468101214mAU020406080100120140N=4904,Tf=1.14SupelcoDiscoveryHS-C18VarianInertsilODS-3N=6327,Tf=1.47N=7256,Tf=1.054132541325WelchUltimateTMXB-C1841325UltimateTMXB-C182:4.6x100mm,5µm:80%/20%20mM,pH7.0:1ml/min:215nm:250C:1)2)3)4)5)147pH1.3:4.6x150mm,5µm:•50%1%TFAinwaterpH1.3/50%Methanol•:3mL/min,:80C,pH=1.3•:500min(1000):•:50%1%TFAinwater,pH1.3/50%Methanol•:1mL/min,•:80C:20minutes•:02040608010012005000100001500020000250003000035000Columnvolumnpurged%ChangeofrelativeretentionUltimateXB-C18AlltechAdsorbosphereHSGlScienceInertsilODS-3