蛋白质组技术方法英文版

蛋白质组学的研究技术Themethodofproteomicsisaboutthreeparts:theseparationoftargetcellsintheorganicorganization,thesegregationofproteininthetargetcellsandtheidentificationofthekindofprotein.So,itformsthreedifferentmainmethodandmanykindsoftechnologytoachievethem.TheMethodofLCMThismethod,shortingforLaserCaptureMicrodissection,isissuedbytheNationalTumourResearchInstitutionofNIHin1996,Itcanaccuratelyseparatethecellswhatweneedforthenextstudyofproteomicsfromtheothercellsmixedinthesameorganization.Thistechnologyisthefirstimportantmethodingainingthetargetcells.TheinstrumentofLCMTheMethodof2-DETheTwo-DimensionalGelElectrophoresis,whichisintroducedintheParagraph6ofourtext,isabbreviatedby2-DE.O’Farrel,Scheele,Kloseandtheircolleaguescreateditin1975.Thistechnology,whichhasbeenacceptedasthemostvaluablemethodsinceitwasinvited,successfullyhelpustodetachallkindsofproteinfromthetargetcells.But,perplex,instability,andlowsensitivityareitsmainweakness.Theinstrumentof2-DEThetheoryof2-DETheMethodofBiomassSpectrometryThismethodbeautifullysolutetheproblemabouthowtoconfirmthespeciesoftheprotein,throughweighingandcalculatingthatproteinmolecule.Itisthelaststageoftheproteomics.Therelativemethodfirstappearedin1886,andthefirstmachinethatisusedinbusinessisputintousein1942.MALDI-TOF-MS(机制辅助激光解吸电离—飞行时间质谱技术)andESI-MS(电喷雾质谱技术)arethemostpopulartechnologyaboutmethodatpresentThemachineofBiomassSpectrometrytheTheoryofthemachineofBiomassSpectrometry