蛋鸡疑似感染大肠杆菌的诊断和综合防治

沈阳农业大学科学技术学院本科毕业论文题目：蛋鸡疑似感染大肠杆菌的诊断及综合防治学院:生命工程学院专业:动物科学学号:1101064011学生姓名:侯爽指导教师:郭文洁2015年05月10日沈阳农业大学科学技术学院毕业论文设计I摘要禽大肠杆菌病是由禽致病性大肠埃希氏杆菌引起各种禽类的局部或全身性感染的慢性或急性传染病，是以引起卵黄性腹膜炎、关节炎、纤维性心包炎、纤维素性腹膜炎以及肉芽肿和输卵管炎等主要特征的疾病。随着养禽业的发展，禽大肠杆菌病已成为危害养禽业的一种常见的、多发的细菌性传染病，环境因素、应激反应等均可导致发病，由于其血清型复杂，细菌的耐药性不断增强，造成污染环境，禽产品质量下降，使世界各国养禽业遭受很严重的经济损失。2014年8月，沈阳法库县某蛋鸡养殖场爆发一场疑似鸡大肠杆菌病的疫病，从而导致的呼吸道疾病，肠炎、卵黄囊炎、腹泻、卵黄性腹膜炎等，导致死亡率升高，产蛋率降低，蛋品质减退。本试验通过检测发病鸡的烟腔拭子、饮水及患病鸡只的肠内容物和粪便，为了对该病进行准确的诊断，对患病鸡只需要进行细菌学检验。试验结果表明，在发病鸡的烟腔拭子、饮水及病死鸡只的粪便和肠内容物中，大肠杆菌数比标准值高于很多，说明其含有大量的大肠杆菌。在对发病鸡只进行的细菌学检验结果表明，其送检样本感染大肠杆菌。根据其流行病学特点、临床症状和病理化特点，进行针对性治疗和支持治疗，有效地控制疾病的传播,减少经济损失。同时，根据该场发病情况，将鸡场划分为非疫区和疫区，分别对针对性的综合防制进行了讨论分析。本研究具有重要的临床意义，为诊断和防治大肠杆菌病提供了重要的理论依据。关键词：禽大肠杆菌病；蛋鸡；诊断；综合防制沈阳农业大学科学技术学院毕业论文设计IIAbstractAviancolibacillosisiscausedbyavianpathogenicescherichiacoliinfectiousdiseaseofavarietyofacuteorchronic,iscausedbyhepaticfiberpericarditis,celluloseperitonitisandyolkperitonitisandofthediseaseofsalpingitisasmaincharacteristic.Aviancolibacillosishasbecomeitwhichcauseseriousdamagetothepoultryindustryanimportantkindofbacterialinfectiousdiseases,duetothecomplexityofitsserotype,drugresistantstrainscontinuetoincrease,hasmadethenationsoftheworldPoultryfarmingindustrysufferedgreateconomiclosses.InJuly2014,Shenyangfakularge-scalepoultyhouseLayerchickenproductionpeakofbroilerbreederinfectioncolibacillosis,whichisleadtorespiratorydisease,diarrhea,vitellineperitonitis,leadingtoincreasedmortality,layingratedecline.Thisexperimentbydetectingtheonsetofhenhouseofdrinkingwater,padding,andsickchickenfecesandintestinalContents,atthesametime,inordertomakeadefinitediagnosisofthediseaseondiseasedchickenbacteriologyinspection.Experimentalresultsshowthat,theonsetofhenhouseofdrinkingwater,padding,andsickchickenfecesandintestinalContents,itcontainsthenumberofe.coliismuchhigherthanstandardvalue.Itshowedthatitcontainsalotofe.coli.Thesickchickenbacteriologyinspection,accordingtotheresultsoftheinspectionsamplesinfectedwithe.coli.Accordingtoitscharacteristicsandtheepidemiologicalcharacteristicsandtakethecorrespondingtreatmentandsupport,toeffectivelycontrolthespreadofdisease,reduceeconomicloss.Atthesametime,divideachickenfarmtopayfortheepidemicareaandthenon-epizooticarea,respectivelyanalyzedthecorrespondingcomprehensivepreventionandcontrolmeasures.Thisstudyhasimportantclinicalsignificance,todiagnosisandtreatmentforescherichiacolidiseaseprovidesanimportanttheoreticalbasis.Keywords：Aviancolibacillosis;Layerchicken;Diagnosis;Comprehensiveprevention沈阳农业大学科学技术学院毕业论文设计1目录摘要....................................................................IAbstract................................................................II1前言....................................................................................................................................41.1病原学.............................................................41.2流行病学...........................................................51.3病理变化...........................................................51.4诊断...............................................................51.4.1病原学的诊断....................................................51.4.2血清学的诊断....................................................61.4.3分子生物学方法..................................................61.5选题依据........................................................................................................................61.5.1养殖概况..................................................................................................................61.5.2发病情况.......................................................61.5.3临床症状........................................................71.5.4主要解剖变化....................................................72材料与方法..........................................................................................................................82.1材料..................................................................................................................................82.1.1病料来源.................................................................................................................82.1.2培养基和血清..........................................................................................................82.1.3实验仪器.................................................................................................................82.2方法..................................................................................................................................82.2.1病死鸡细菌学检测...................................................................................................82.2.2饮用水细菌学检测...................................................................................................82.2.3细菌的分离培养.....................................................................................................82.2.4细菌形态学镜检.....................................................................................................92.2.5生化试验.....................................................................................