脂肪源性基质细胞体外诱导分化为星形胶质细胞障碍

Apoptosisisanobstacletothedifferentiationofadipose-derivedstromalcellsintoastrocytesXiaodongYuan1,QiaoyuSun1,YaOu1,ShujuanWang1,WenliZhang2,HongliangDeng1,XiaoyingWu1,LiliZhang11DepartmentofNeurology,KailuanGeneralHospital,HebeiUnitedUniversity,Tangshan,HebeiProvince,China2DepartmentofElectronMicroscopy,HebeiUnitedUniversity,Tangshan,HebeiProvince,China摘要既往研究发现脂肪源性基质细胞经化学诱导后，分化的神经细胞存活时间较短，原因有待证实。实验应用化学诱导剂诱导成人脂肪基质细胞分化为星形胶质细胞，经MTT和流式细胞仪检测，随诱导时间的延长，分化细胞中细胞凋亡率逐渐增加，存活细胞数量逐渐减少。免疫组化染色显示，随着诱导时间的延长，细胞中GFAP，caspase-3和caspase-9阳性细胞数量逐渐增加。透射电镜观察到分化后的细胞部分出现典型的细胞凋亡特征。表明caspase依赖性细胞凋亡是脂肪源性基质细胞分化为星形胶质细胞的障碍，提示抑制这种细胞凋亡可能是改善诱导星形胶质细胞效果的重要措施。关键词：神经再生；成人脂肪源性基质细胞；细胞凋亡；caspase依赖性细胞凋亡；细胞分化；星形胶质细胞；caspase-9；caspase-3；Acceptancedate:2014-03-28Citethisarticle:XiaodongYuan,QiaoyuSun,YaOu,ShujuanWang,WenliZhang,HongliangDeng,XiaoyingWu,LiliZhang.Apoptosisisanobstacletothedifferentiationofadipose-derivedstromalcellsintoastrocytes[J].NeuralRegenerationResearch,2014,9(8):837-844.