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当前位置:首页 > 办公文档 > 工作计划 > 四种增色剂对锦鲤的生长-形体-体色和抗氧化能力的影响
200916,。,,。(1999),。(2005),,。,。、,,。11.11.1.1。、、*[]、、、、、。1050(19.28±0.51)g14,3,25,3(50、100、200mg/kg),400mg/kg,60d。:(1)、、(P0.05),(P0.05);(2)、;(3):200mg/kg100mg/kg100mg/kg200mg/kg。,,,200mg/kg。[];;;;;[]S963.73[]A[]1004-3314(2009)16-0033-04[Abstract]Thisstudywasconductedtoevaluatetheeffectsofdietsadded4kindsofcolorants(astaxanthin,lutein,apo-esterandcapsanthin)ongrowth,morphologicalindices,body-colorandantioxidantcapacityofredkoicarpwithaver-ageinitialweightof(19.28±0.51)g.1050redkoicarpwasrandomlydiviedeinto14treatmentsof75each,25foreachreplicate.Thelevelof4colorantsaddingintothebasaldietswere50,100mg/kgand200mg/kg.Adietwithoutcolorantssupplementservedascontrolandadietcontained400mg/kgastaxanthinwasincluded.After60daysoffeeding,there-sultsshowedthat:(1)Colorantshadremarkablyeffectonthegrowth,body-colorandantioxidantcapacityofkoicarp(P0.05),butnosignificantonmorphologicalindices(P0.05);(2)Theweightgain(WG),specialgrowthratio(SGR)andantioxidantcapacityofcolorantgroupswerelowerthanthoseofcontrolgroup;(3)Thesequenceoftotalcarotenoidscon-tentinbodysurfaceofkoicarpwas:capsanthin200mg/kggroupcapsanthin100mg/kggroupapo-ester100mg/kggrouplutein200mg/kgtheothercolorantgroupscontrolgroup.Accordingtotheresults,inconsiderationofthecom-petitivepriceandbettereffect,capsanthinof200mg/kgaddedisoptimumtoimproveredkoicarpbody-colorintheex-periment.[Keywords]koicarp;colorant;growth;morphologicalindices;body-color;antioxidantcapacity:“”(ZX[2007]031);“”(G[2007]4010)*33200916,、。1.1.2:,8%,;,20%,;,10%,;,20%。1。1.21050(19.28±0.51)g14,3,25,。50、100、200、400mg/kg、50、100、200mg/kg、50、100、200mg/kg、50、100、200mg/kg,()。(1m×0.5m×0.5m),60d。3(08∶00,13∶0018∶00),3%~5%,。25~30℃,5~6h,5mg/L,pH6.6~7.2,0.5mg/L。1.31.3.1、,24h,/、/、、、、、、、,:(WG)=(Wt-W0)/W0×100%;(SGR,%/d)=(lnWt-lnW0)×100/d;/(L/T)=L/T;/(W/L)=W/L;(CF)=W/L3×100%;(VIS)=Wn/W×100%;(DOR)=(W-Wn)/W×100%;W0,g;Wt,g;Wn,g;d,d;L,cm;T,cm;W,g。1.3.23,,4℃24h。24h。,0.4g,28mL(V∶V=2∶1),,8h。,(4000r/min)10min,,5%,15mL,。10g,10mL,,10mL,。1cm,,448nm,(,2008)。:X=A×y×106A%1cm×1000×g;X,mg/100g;A448nm;y,mL;A%1cm2500(Jeffrey,1997);g。1.3.3(SOD),TBA(MDA),T-AOC(T-AOC),。,。1.4±,SPSS11.51%/%7/%17/%8/%10/%23/%2/%23/%2/%7/%1*/%10.02/%4.96/%30.78/%11.71:(1)Fe150mg,Cu3.2mg,Zn34.1mg,Mn13.0mg,I5.7mg,Se0.3mg,Co12.4mg;A2000.0IU,D2000.0IU,E100.0mg,K310.0mg,B15.0mg,B210.0mg,100.0mg,B610.0mg,40.0mg,5.0mg,B120.02mg,1.0mg,C300mg;(2)*。342009162(W0)/g(Wt)/g(WG)/%(SGR)/(%/d)19.41±0.3651.62±1.08166.00±2.22g1.63±0.01gA-50mg/kg18.53±0.5645.43±1.21158.78±1.38def1.49±0.01defA-100mg/kg19.71±0.3748.61±1.06146.58±2.89ef1.50±0.02efA-200mg/kg19.16±0.5947.75±1.00149.39±7.57f1.52±0.05fA-400mg/kg19.25±0.6549.86±1.59159.01±2.48g1.59±0.02gL-50mg/kg19.38±0.6846.55±1.59140.22±1.92bcde1.46±0.01bcdeL-100mg/kg19.59±0.5246.45±3.02137.01±9.29bc1.44±0.07bcL-200mg/kg18.99±0.2444.53±0.31134.50±3.99ab1.42±0.03abAE-50mg/kg19.98±0.1948.57±0.87143.06±2.79cdef1.48±0.02cdefAE-100mg/kg19.82±0.1147.12±0.75137.74±2.85bcd1.44±0.02bcdAE-200mg/kg18.92±0.2044.59±0.34135.69±1.09bc1.43±0.01bcdC-50mg/kg18.92±0.7844.94±1.38137.64±6.97bcd1.44±0.05abcC-100mg/kg19.90±0.1945.26±0.40127.47±1.14a1.37±0.01aC-200mg/kg19.39±0.5644.14±0.99127.61±2.31a1.37±0.02a:(1)A-,L-,AE-,C-,。(2),(P0.05);(P0.05);。3/(L/T)/(W/L)(CF)/%(VIS)/%(DOR)/%2.91±0.204.06±1.162.61±0.196.48±0.7693.52±0.76A-50mg/kg2.83±0.173.56±0.762.87±0.466.90±0.3493.10±0.34A-100mg/kg2.96±0.153.67±0.942.73±0.226.60±0.8193.40±0.81A-200mg/kg2.94±0.254.18±0.582.77±0.186.72±0.3593.28±0.35A-400mg/kg2.86±0.233.58±0.622.83±0.276.56±0.5993.44±0.59L-50mg/kg2.93±0.093.50±0.672.64±0.166.93±0.2593.05±0.68L-100mg/kg2.96±0.113.72±0.482.66±0.246.39±0.8393.61±0.83L-200mg/kg2.80±0.193.60±0.762.87±0.236.72±0.3493.28±0.34AE-50mg/kg2.89±0.183.71±0.722.76±0.336.36±0.4693.64±0.46AE-100mg/kg2.91±0.114.61±0.952.91±0.766.24±0.4693.76±0.46AE-200mg/kg2.84±0.094.31±1.282.90±0.196.28±0.7593.72±0.75C-50mg/kg2.91±0.173.21±0.872.70±0.246.15±0.4093.85±0.40C-100mg/kg2.94±0.163.94±1.652.72±0.216.35±0.3593.88±0.56C-200mg/kg2.89±0.154.63±2.012.90±0.286.24±0.6493.76±0.644/(mg/100g)6.10±0.13aA-50mg/kg8.42±0.06bA-100mg/kg11.27±0.15cA-200mg/kg11.20±0.02cA-400mg/kg12.19±0.08dL-50mg/kg13.97±0.03hL-100mg/kg13.79±0.04gL-200mg/kg14.07±0.08hAE-50mg/kg13.72±0.01gAE-100mg/kg15.38±0.07iAE-200mg/kg12.86±0.10fC-50mg/kg12.58±0.08eC-100mg/kg16.48±0.11jC-200mg/kg18.04±0.02k,Duncan’s。22.12,(P0.05);,50、100mg/kg200mg/kg(P0.05),400mg/kg(P0.05),;(P0.05),;(P0.05),;50mg/kg100mg/kg200mg/kg(P0.05),(P0.05),。2.23,(P0.05)。2.34,,(P0.05)。100mg/kg200mg/kg(P0.05),(P0.05),;50mg/kg200mg/kg(P0.05),,;(P0.05),;(P0.05),,。:(200mg/kg)(100mg/kg)(200mg/kg)(400mg/kg)。2.45,SOD,T-AOC。,T-AOCSOD,MDA;,T-AOCSOD,MDA;T-AOC352009165T-AOC/(U/mgprot)SOD/(U/mgprot)MDA/(nmol/mgprot)2.28±0.09h395.10±1.40l9.74±0.09hA-50mg/kg1.04±0.06bc296.78±2.57d11.28±0.09lA-100mg/kg1.04±0.10bc299.50±1.30e10.70±0.06jA-200mg/kg1.62±0.11f322.53±0.98g9.84±0.08hA-400mg/kg1.95±0.07g344.10±1.50k9.41±0.06gL-50mg/kg3.02±0.06i178.04±1.24c6.76±0.05eL-100mg/kg1.70±0.11f142.14±1.00b10.51±0.07iL-200mg/kg0.96±0.04ab103.28±1.52a11.05±0.08k
本文标题:四种增色剂对锦鲤的生长-形体-体色和抗氧化能力的影响
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