胶体金免疫层析技术的研究进展-张莹

△11213100142300072、、、、、。、、。、。R392-33A1673-6273201512-2349-03ProgressoftheColloidalGold-basedImmunochromatographicAssayZHANGYing1,FENGHai1△,WUQiu-ping1,TANZeng1,HEJia-bei2(1Collegeofpharmacy,ZhejingUniversityofTechnology,Hangzhou,Zhejiang,310014,China;2TheSchoolofPharmaceuticalScienceandTechnology,TianJinUniversity,Tianjin,300072,China)ABSTRACT:Colloidalgold-basedimmunochromatographicassayisacommonusedlateralflowimmunoassay,whichofferstheadvantageofsimplicity,rapidity,relativelylowmanufacturingcostsandeasyevaluation,anditisideallysuitedforonsitetesting,andwidelyusedinbiology,medicine,foodandotherfields.Buttherearesomedisadvantages,suchas,lowsensitivity,beingdifficulttoqualitycontrolhardtoquantitativedetectionandsoon.Theshortcomingslimititsapplicationinacertainextent.Thispaperdescribestheprinciple,characteristicsanddomesticandforeignprogressofthemethod,andemphasizesonitslacksandthepossibilitiesforimprovement,soastohelptobroadenitsarrayofnewapplicationareas.Keywords:Colloidalgold;GICA;Sensitivity;Quantitativedetection;MultipledetectionChineseLibraryClassification(CLC):R392-33Documentcode:AArticleID:1673-6273(2015)12-2349-031988-13958029139E-mail:[e-mail]△0571-88320776E-mail:[e-mail]2013-10-152013-11-10(Colloidalgold-basedimmunochromato-graphicassay,GICA、[1]、、、、。、、/[2]。、、、。、GICA。1。20~40nm[3]。。GICA。GICA、、、。。。。290Beggs[4]GICA(humanchorionicgonadothphin,hCG)。①、②、ELISAGICA5~15min③2349··④、、、、、。GI-CA、。3、、。3.1。、、。。WhatmanFusion5TM、、、[5]。。。。[6][7-9]。3.2GICAELISA。、、、。、。GICA-。-。。、、。。10μm[6]。、。Horton[10]Tween-205GICA。NaokiNagatani[11]。RyoTanaka[12]GICA。ClaudioParolo[13](HRP)HRP。DongYang[14]Fe3O4/AuGICA。3.3[15]。1、、2[1]3、。。。QIAGENESE-Quant60[16]SkannexSkanFlexi[17]。3.4。[18]。ZhangCan[19]。Taro2350··[20]3。GICAAdvntBiotech-nologiesProStripsTM[21]5。3.5、。。、、。、[7]。PallVividTMCy-tosepTM。Demecal誖、[22]。、、。。4、、、[23]。、。、、、。GICA、、、、、、、、、、。(References)[1],.[J].,2013,42(1):91-93LinYi-Ming,WangYun-Long.Progressintheclinicaldiagnosisofthecolloidal-goldimmunochromatographyassay[J].JChongqingMedical,2013,42(1):91-93[2]Posthuma-TrumpieGA,KorfJ,vanAmerongenA.Lateralflow(immuno)assay:itsstrengths,weaknesses,opportunitiesandthreats.Aliteraturesurvey[J].Analyticalandbioanalyticalchemistry,2009,393(2):569-582[3]ChandlerJ,GurminT,RobinsonN.Theplaceofgoldinrapidtests[J].IVDTechnology,2000,6(2):37-49[4]BeggsM,NovotnyM,SamocdroS,etal.Aselfperformingchromatographicimmunoassayforthequalitativedeterminationofhumanchorionicgonadotrophininurineandserum[J].ClinicalChemistry,1990:1084-1085[5]RaphaelW,HarleyT.Lateralflowimmunoassay[M].NewYork:HumanaPress,2009:115-129[6]O'FarrellB,BauerJ.Developinghighlysensitive,morereproduciblelateralflowassays.Part2:Newchallengeswithnewapproaches[J].IVDTechnology,2006,12(6):67-75[7]DavidW.TheImmunoassayHandbook[M].4thed.Oxford:Elsevier,2013:89-107[8]TisoneTC.In-linemanufacturingforrapid-flowdiagnosticdevices[J].IVDTechnology,2000,6(3):43-60[9]TisoneTC.In-lineprocessingtrendsforlateral-flowimmunoassaymanufacturing[J].IVDTechnology,2007,13(5):37-41[10]HortonJK,SwinburneS,O'SullivanMJ.Anovel,rapid,single-stepimmunochromatographicprocedureforthedetectionofmouseimmunoglobulin[J].JournalofImmunologicalMethods,1991,140(1):131-134[11]NagataniN,TanakaR,YuhiT,etal.Goldnanoparticle-basednovelenhancementmethodforthedevelopmentofhighlysensitiveimmunochromatographicteststrips[J].ScienceandTechnologyofAdvancedMaterials,2006,7(3):270-275[12]TanakaR,YuhiT,NagataniN,etal.Anovelenhancementassayforimmunochromatographicteststripsusinggoldnanoparticles[J].Analyticalandbioanalyticalchemistry,2006,385(8):1414-1420[13]ParoloC,delaEscosura-MuizA,MerkoiA.Enhancedlateralflowimmunoassayusinggoldnanoparticlesloadedwithenzymes[J].BiosensorsandBioelectronics,2012,40(2013):412-416[14]YangD,MaJ,ZhangQ,etal.Polyelectrolytecoatedgoldmagneticnanoparticlesforimmunoassaydevelopment:towardpointofcarediagnosticsforsyphilisscreening[J].Analyticalchemistry,2013,85:6688-6695[15]RaphaelW,HarleyT.Lateralflowimmunoassay[M].NewYork:HumanaPress,2009:157-183[16]QIAGEN.ESEQuantLateralFlowImmunoassayReader[EB/OL].[2013-10-10].[17]Skannex.SkanFlexi[EB/OL].[2013-10-10].[18].[J].,2011,28(4):36-39QiYing-ying.Immunecolloidalgoldtechniqueanditsapplications[J].JournalofHeNanForestryScienceandTechnology,2011,28(4):36-39[19]ZhangC,ZhangY,WangS.Developmentofmultianalyteflow-throughandlateral-flowassaysusinggoldparticlesandhorseradishperoxidaseastracersfortherapiddeterminationofcarbarylandendosulfaninagriculturalproducts[J].JournalofAgriculturalandFoodChemistry,2006,54(7):2502-2507[20]YonekitaT,OhtsukiR,HojoE