五种荨麻属植物提取物抑制前列腺增生作用的研究

1Effectsof5UrticaplantsonBenignProstaticHyperplasiaBaominFeng*LiyingShiLingTangYongqiWangCollegeofBioengineering,DalianUniversity,Dalian,116622,China五种荨麻属植物提取物抑制前列腺增生作用的研究冯宝民*史丽颖唐玲王永奇大连大学生物工程学院，大连市经济技术开发区，116622摘要：目的：对5种荨麻属植物(裂叶荨麻、三角叶荨麻、滇藏荨麻、狭叶荨麻、宽叶荨麻)的地上和地下部分的20%乙醇提取物进行抑制前列腺增生作用的筛选。方法：采用去势大鼠肌肉注射丙酸睾酮注射液复制了大鼠前列腺增生病理模型，造模动物灌胃给予受试药物，连续24天，剖取前列腺称重并进行病理学检查，镜下观察前列腺中卵磷脂小体密度，同时取血清检测酸性磷酸酶含量。结果：这5种荨麻属植物的地上和地下部分的提取物大多对模型动物前列腺增生表现抑制出抑制作用；其中裂叶荨麻地上和地下部分、滇藏荨麻的地上部分、狭叶荨麻的地下部分以及宽叶荨麻的地上部分提取物可明显增加前列腺中卵磷脂小体密度；对血清中酸性磷酸酶含量影响各组同模型组比较均无统计学意义，但其中裂叶荨麻地上和地下部分、滇藏荨麻的地上部分提取物有一定减少趋势（t1.6）。组织病理学检查发现，裂叶荨麻地上部分、滇藏荨麻的地下部分、狭叶荨麻的地上和地下部分以及宽叶荨麻的地上部分对病理改变程度有减轻趋势。结论：综合各项指标发现，裂叶荨麻和滇藏荨麻的地上部分作用最强，有望开发成为抗前列腺增生的药物。关键词：荨麻属植物，抗前列腺增生，酸性磷酸酶AbstractObjective:Tostudytheeffectsof20%ethanolextractsofaerialandundergroundpartsof5Urticaplants[UrticafissaE.Pritz,UrticatriangularisHand-Mass,UrticamaireiLevl,UrticaangustifoliaFisch.ExHornemandUrticalaetevirensMaximsubsp.dentate(Hand-Mazz.)C.J.Chen.Urticaceae]againstbenignprostatichyperplasia(BPH)wereobservedinthispaper.Method：Eachextractwasadministratedorallyfor24dayscontinuouslyaftertheestablishmentoftheratsexperimentalprostatichyperplasiamodelwhichwasinducedbytheinjectionoftestosteronepropionateincastratedrats.Prostatesofratswerebisectedandweighedandthedensityoflecithincorpusclewasobservedundermicroscope.Seraofratswerepreparedandthecontentsofacidphosphatase(ACP)inwhichweredetected.Resultsanddiscussion:MostextractsofUrticaplantshaveinhibitoryactivitiesagainstBPHindifferentdegrees.Particularly,extractsofaerialpartofUrticafissaE.PritzandUrticamaireiLevlwerethemosteffectiveandmightbeusedasagentsinnewdrugsformenwithmildtomoderateBPH.Thispapermaybethefirstdocumentationofthese5urticaplantsinvivoagainstBPHactivity.Keywords:Urticaplants,benignprostatichyperplasia,acidphosphataseUrticaplantsareperennialherbs,growinginnitrogen-richsoils,andwidespreadinChina.InfolktheyareusedtotreatBPH,hypotensiveandantidiabetic[1-3].Duringthelatestyears,numerouspapershave*通讯作者简介：男，1975.8出生，副教授，大连大学生物工程学院副院长，博士，毕业于沈阳药科大学，主要从事天然药物化学研究Tel:0411-87403834Fax:[phone]E-mail:[e-mail]2beenpublishedwhichelaboratedthepharmacologicalactivitiesandtheclinicalassessmentsoftheseherbalremedies[4,5].U.dioicaL.(Urticaceae)wasthemostdeeplystudiedspeciesofthisgenusbecauseofitsmarkedbiologicalactivities.BPHisthemostcommondisordersinelderlymen.Inmanycountriestheusageofphytopharmaceuticals,particularlytherootsofstingingnettle,forthetreatmentofBPHiscommon[6,7].ChinahasabundantresourcesanduniquespeciesofUrticaplants,butthepharmacologyandphytochemistryarepoorlystudied.Inthispaper,weaimedtodeterminetheactivitiesagainstBPHofseveralChineseUrticaplants.1MaterialsandMethods1.1PlantmaterialsandextactionU.laetevirensMaximsubspdentatewascollectedinJilinprovinceChina,July2004andtheother4UrticaplantswerecollectedinSichuanprovinceChina,October2004.ThevoucherspecimensauthenticatedbyProf.CChen(LiaoningNormalUniversity,Dalian,China)weredepositedinCollegeofBioengineeringofDalianUniversity.Eachsampleofthedriedplantswasdividedintoaerialandundergroundparts(exceptU.laetevirensMaximsubsp.withonlyaerialparts)andcutintopiecesrespectively,thenwasextractedwith20%ethanolatroomtemperature,subsequentlythefilteredextractsevaporatedtodrynessinvacuoat40℃toget9extracts(1and2:aerialandundergroundpartsextractsofUrticafissaE.Pritz,3and4:thoseofUrticatriangularisHand-Mass,5and6:thoseofUrticamaireiLevl,7and8:thoseofUrticaangustifoliaFisch.ExHornem,9:aerialpartextractofUrticalaetevirensMaximsubsp.).Testedsamplesweredissolvedin2%aqueousTween80inspecificdosesasdescribedlater.1.2ReagentsTestosteronePropionateInjectionproducedbyShanghaiGeneralPharmacerticalCo.Ltd(China).BatchNo:040303[solvedinplantoil(0.25)foruse].ProstatTablets(adrugfortreatBPHinclinic)producedbyNanjingMeiRuiPharmaceuticalCo.,Ltd(China).BatchNo:20040503.AcidphosphataseassaykitwaspurchasedfromTECODiagnostics,CALIF(U.S.A).1.3AnimalsMaleWistaralbinorats,weighing150–210g,wereused.AllanimalsobtainedfromtheAnimalHouse,ChangchunMedicalAnimalCenterofExperimentandResearch[Certificate：SCXK-(Ji)2003-0004,China],weremaintainedinastandardenvironmentalconditions.Theywerefedwithstandardrodentdietandwateradlibitum.1.4InstrumentsTheamountoftotalacidphosphataseandnon-prostaticacidphosphatasewereanalyzedbyacidphosphataseassaykitusingAuto-biochemicalAnalyser(China,ShangHaiSanjoseMedicalProductsLtd.).ThedensityoflecithincorpusclewasobservedbyOlympus2700microscope(OlympusChinaCo.,Ltd.).TheprostateswereweighedusingLE324Selectronicbalance(GermanySartoriusCo.,Ltd).1.5MethodsTheactivityagainstBPHwasmeasuredthroughslightlymodifyingthemethoddescribedbyliteratures[8,9].Theratswereanesthetizedbyintroperitonealinjectionofchloralhydrate(3%,10mg/kg)andthetesteswereremoved.Afterfedfor1week,theratswereinjectedTestosteronePropionateInjection(ie,2ml/kg,onceadayfor24dayscontinuously).Thecastratedratswereclassifiedinto20groups(includingcontrolgroup,positivegroupand18samplegroups,animalnumberseeTable1)randomly.Theratswiththesham-operationweretakenasnormalgroup.3Eachgrou