不同废水基质条件下微生物燃料电池中细菌群落解析

2008,28(12)1068~1073ChinaEnvironmentalScience1,2*,2,2,2(1.,1008752.,100084)1(MFC),2,,.435mV,475mV,2,COD60%.16SrRNA,.,,,G+C,β(β-proteobacteria)δ(δ-protecobacteria).(Geobacteraceae).(MFC)X703.5A10006923(2008)12106806Analysisofmicrobialdiversityinmicrobialfuelcellsunderdifferentwastewater.SUNYu-jiao1,2*,ZUOJian-e2,CUILong-tao2,DANGYan2(1.CollegeofWaterSciences,BeijingNormalUniversity,Beijing100875,China2.DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,China).ChinaEnvironmentalScience,2008,28(12)1068~1073AbstractAsmallmicrobialfuelcell(MFC)wasoperatedcontinuouslywithtwodifferentinfluent,inwhichtheCODwasremovedefficientlyandelectricitywasgeneratedsimultaneously.TheexportedvoltagesforMFCwereabout435mVforMFCfedbyglucoseand475mVfedbyanaerobiceffluence,whileCODremovalefficiencieswereallabove60%.With16SrRNAgenomiclibraryconstructionandrandomsequencing,thediversityofmicrobesonanodessurfaceweredetectedrespectively.Eubacteriacommunitystructuresonanodesurfaceschangedgreatlybydifferentinfluent,butseveralrelativeelectrochemicallyactivemicrobessuchaslowG+Cgrampositivebacteria,β-proteobacteriaandδ-proteco-bacteriadidnotchangemuch.ThepossiblehighelectrochemicallyactivebacteriabelongtoGeobacter.Keywordsmicrobialfuelcells(MFC)electrochemicallyactivemicrobesanodecommunitystructuresubstance(MFC),,,[14].,.MFC,,,COD[57].Shewanellaputrefaciens,[1,8].,.1MFC,2,,.,,MFC.20080611(2005038073)*,,[e-mail]12106911.1MFCMFC,(100mm×100mm×110mm),1.11L.,5,350mm×50mm250mm×80mm,.1(1mg/cm2),50mm×50mm,,.,..,MFC,1000Ω.COD,,1,(1).1MFCFig.1SchematicpresentationofMFC1.2.:30mL,,,20mL,6h.,30min,MFC.1.31.3.1(A),.COD400mg/L,10mL,50mL,pH,.MgSO4(10g/L),CaCl2(1.5g/L),FeCl3·6H2O(0.1g/L),MnSO4·H2O(2g/L),Na2HPO4·12H2O(177.99g/L),NH4CO3(67g/L),KH2PO4(67.8g/L).1.3.2(B)MFC,(),COD300~400mg/L.(COD100mg/L),MFC,,.2MFC.ABMFC.2,MFC15d.1.41.4.1DNAPCRDNA,FastprepBiorad.DP301DNA,DNA,A260/A2801.77,DNA.DNA,8F/1492R(5′AGAGTTTGATCCTGGCTCAG3′/5′GGTTACCTTGTTACGACTT3′)[7]16SrRNA,1%PCR,(A0124).1070281.4.216SrDNADNA16SrDNApGEMT(PromegapGEM®TVectorSystemI);E.coliDH5α.(100µg/mL)[9],,PCR.PCR.16SrDNA,RsaІMspІ;16SrRNA,RsaІTaqІ[10].3%,,.16SrDNA.(OTU).OTUBLASTNCBI,.,DNAMAN(Version4.1),,Bootstrap.22.1MFC0.000.050.100.150.200.250.300.350.400510152025303540455055(d)[kg/(m3·d)]0100200300400500600(mV)2AMFCFig.2VolumeloadandoutputvoltageofMFCwithwastewaterAA,COD300mg/L,250mL/d,4d,0.074kg/(m3·d).530mV(2),COD100mg/L,COD60%~70%.B,,COD300mg/L,4d,0.15kg/(m3·d).475mV(3).COD100mg/L,COD60%~70%.00.050.100.150.200.25135791113151719(d)[kg/(m3⋅d)]0100200300400500(mV)3BMFCFig.3VolumeloadandoutputvoltageofMFCwithwastewaterB2.2AA,4,40OTUS64(1),NCBI,(98%)6:G+C,G+C,β-proteobacteria,γ-proteobacteria,δ-proteobacteria,Greennonsulfurbacterium.G+Cγ-proteobacteria,34.8%37.5%.G+C,;γ-ProteobacteriaKlebsiellaoxytoca.2.3BB,22OTUS64(1).NCBI,(98%)8(5):G+C121071,Spirochaetes,SRI,OpII,CFBBacteroidetes,β-proteobacteria,δ-proteobacteria,ε-proteobacteria,A.ε-proteobacteriaG+C,57.81%17.19%.ε-proteobacteria;G+CTrichococcusClostridium.2.4MFC,AG+Cγ,,,;Bε,,,[11].unculturedbacteriumAY935648OUTA3(1/64)OUTA1(5/64)unculturedbacteriumDQ093953OUTA2(1/64)Lactococcussp.MKRL19AY762104LactococcuslactisAY675242NostocoidalimicolaIAF255736OUTA4(11/64)Bacteriumsp.A15AY570695OUTA5(11/64)OUTA9(4/64)CitrobacterfreundiiAY186052OUTA12(2/64)RaoultellaornithinolyticaKOY17662OUTA13(1/64)OUTA11(1/64)KlebsiellaoxytocaAY292871OUTA8(6/64)PantoeaagglomeransAY691545OUTA10(6/64)BacteriumG3GreenLakeAY345394Aeromonassp.T8AF099027AeromonasveroniiAF170914OUTA15(1/64)PseudomonassynxanthaAY486386OUTA14(3/64)PseudomonasfluorescensAY622221Janthinobacteriumsp.PI12DQ202711OUTA6(1/64)OUTA7(1/64)ArcticsoilbacteriumK2LI3DQ234463OUTA18(3/64)unculturedGeobactersp.AF404348GeobactergrbiciaeAF335183Desulfobulbussp.RPf35L17AY548775OUTA16(3/64)OUTA17(2/64)DesulfobulbuselongatusX95180unculturedsoilbacteriumAY989345OUTA19(1/64)unculturedChlorobibacteriumAY118152MethanosaetaconciliiX1693278975047802310066100100661001007710085100100100616610056921001001001008410010053981009453951000.05GNSδ-protebacteriaβ-protebacteriaγ-protebacteriaG+CG+G+CG+4AFig.4EubacteriaphylogenetictreeofsampleA107228OUTB13(1/64)unculturedbacteriumAB189686OUTB14(1/64)unculturedbacteriumAB237703OUTB15(1/64)unculturedbacteriumCR933202OUTB16(1/64)unculturedbacteriumU81676OUTB11(1/64)unculturedbacteriumAB247481OUTB1(2/64)unculturedanaerobicbacteriumAY953206OUTB2(1/64)unculturedbacteriumAF371776OUTB3(3/64)unculturedbacteriumCR933290OUTB4(1/64)Clostridiumsp.strainP6AY949857OUTB6(1/64)unculturedbacteriumAY570590OUTB5(1/64)unculturedbacteriummle1-9AF280848OUTB7(1/64)TrichococcusflocculiformisAJ306611OUTB8(1/64)StreptococcusbovisAY347306OUTB9(1/64)Spirochaetasp.BuddyAF357916OUTB10(1/64)unculturedbacteriumSJA-88AJ009479OUTB20(1/64)unculturedbacteriumCT573985OUTB20(1/64)OUTB21(2/64)GeobactergrbiciaeAF335183GeobacterpickeringiiDQ145535AlcaligenesmonasteriensisDQ469586OUTB19(1/64)Alcaligenessp.80AY672759ComamonasdenitrificansAF233880OUTB18(2/64)Dechloromonassp.R-28400AM084133OUTB17(1/