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2010,26(7)ChineseJournalofZoonoses:1002-2694(2010)07-0651-03中国致病性小肠结肠炎耶尔森菌生物分型研究肖玉春,王鑫,邱海燕,景怀琦:初步了解我国致病性小肠结肠炎耶尔森菌O3O9血清型菌株的生物型分布,掌握我国致病性小肠结肠炎耶尔森菌的生物学特征用文献报道方法对1986-2008年从我国不同地区的腹泻病人家禽家畜鼠类食品环境等各类宿主中分离到的427株致病性小肠结肠炎耶尔森菌进行生物分型427株致病性小肠结肠炎耶尔森中213株属于生物2型(49.9%),全部为O9血清型;208株属于生物3型(48.7%),其中191株为O3血清型,17株为O9血清型;6株属于生物4型(1.4%),全部为O3血清型没有发现生物1A型1B型与5型的致病性菌株我国O3血清型致病性小肠结肠炎耶尔森菌以生物3型为主O9血清型致病性菌株以生物2型为主,没有生物1B型菌株,与国外致病性菌株的生物血清型分布有一定差异:致病性小肠结肠炎耶尔森菌;血清型;生物型:R378:AStudyofBiotypingforpathogenicY.enterocoliticastrainsinChinaXIAOYUchun,WANGXin,QIUHaiyan,JINGHuaiqi(InstituteofCommunicableDiseaseControlandPrevention,ChineseCenterforDiseaseControlandPreventiom,Beijing102206,China)ABSTRACT:ToanalysesthedistributionandcharacteristicsofbiotypeO3andO9ofY.enterocoliticastrainsinChina,pathogenicY.enterocoliticastrainsisolatedfromdiarrheapatients,domesticanimals,murine,foodandenvironmentwerebiotypedaccordingtoreportedmethods.Among427strains,213(49.9%)werebiotype2,208(48.7%)werebiotype3,and6(1.4%)werebiotype4,whilenobiotype1A,1Band5werefound.It'sconcludedthatinChina,biotype3wasthedominateinO3serotypeofY.enterocoliticaandbiotype2wasthedominateinO9serotype.Thereisnobiotype1BinChina,whichisdifferentfromthedistributionofpathogenicY.enterocoliticabioserotypeinothercountries.KEYWORDSPathogenicY.enterocolitica;serotype;biotype通讯作者:景怀琦,Email:jinghuaiqi@icdc.cn:中国疾病预防控制中心传染病预防控制所,北京102206,,,,,(PFGE),,13!,30,11.1:,,1986-2008,427:52203(O3)52211(O8)52212(O9)O5,27D92651ChineseJournalofZoonoses2010,26(7)1.2O3O9O8,O5,27,O3O8O91.334!,,1.42!:11A1B2345++----+-----+-----++(+)---++++-d+++++-+++++-DNA----++d-----D+-----+-----:+:90%;d:11%-98%;-:90%;(+)::1%800.01%(CaCl2∀H2O)(),35#4d,()(Oxoid,),25#24h1A,;(Rosco,):,OD3.3(),1.5mLeppendorf,250L,,25#,22.1O3O8O941B2,O5,2732.2427,2132,49.9%;2083,48.7%;64,1.4%1A1B52O3O9197O3,1913,643O3(3/O3),O397.0%;O34(4/O3),3.0%230O9,2132(92.6%),173(7.4%),2O9,1B5!21A1B2345O31916O921317213208652203(O3)+52211(O8)+52212(O9)+D92(O5,27)+3,(PFGE),,606!,O36522010,26(7)ChineseJournalofZoonosesO9O5,27O8O13aO13bO20O212!586!,O3O94,7!,O8,23,5!,3061A8!(HPI)1B,O8,O211B5!234523!,23,419801B,O8,HPI(bioserotype),:∃4/O3%∃4/O3%,4/O3,1B/O8,1B/O82/O923!,3/O32/O9,4/O3,:1!CoverTLandAberRC.YersiniaenterocoliticaJ!.NEnglJMed,1989,321:1624.2!BottoneEJ.Yersiniaenterocolitica:thecharismacontinuesJ!.ClinMicrobiolRev,1997,10:257276.3!ThoernerP,BinKingombeCI,BogliStuberK,etal.PCRdetectionofvirulencegenesinYersiniaenterocoliticaandYersiniapseudotuberculosisandinvestigationofvirulencegenedistributionJ!.ApplEnvironMicrobiol,2003,69:18101816.4!WangX.,QiuH,JinD,etal.O:8serotypeYersiniaenterocoliticastrainsinChinaJ!.IntJFoodMicrobiol,2008,125:259266.5!PelludatC,RakinA,JacobiCA,etal.TheyersiniabactinbiosyntheticgeneclusterofYersiniaenterocolitica:organizationandsiderophoredependentregulationJ!.JBacteriol,1998,180:53846.6!M!.,2004.7!,,,.O:3O:9J!..2004,15:317319.8!SinghI,VirdiJS.ProductionofYersiniastabletoxin(YST)anddistributionofystgenesinbiotype1AstrainsofYersiniaenterocoliticaJ!.JMedMicrobiol.2004,53:10651068.:20100126;:20100317(上接第650页)13!StoffelsJA,LeeuwenWM,PostRJ.DetectionofplasmodiumsporozoitesinmosquitoesbypolymerasechainreactionandoligonucleotiderDNAprobe,withoutdissectionofthesalivaryglandsJ!.MedVetEntomol,1995,9(4):433437.14!MorenoM,CanoJ,NzamboS,etal.MalariapanelassayversusPCR:DetectionofnaturallyinfectedAnophelesmelasinacoastalvillageofEquatorialGuineaJ!.MalarJ,2004,3:20.15!VythilingamI,NitiavathyK,YiP,etal.Ahighlysensitive,nestedpolymerasechainreactionbasedmethodusingsimpleDNAextractiontodetectmalariasporozoitesinmosquitosJ!.SoutheastAsianJTropMedPublicHealth,1999,30(4):631635.16!TassanakajonA,BoonsaengV,WilairatP,etal.PolymerasechainreactiondetectionofPlasmodiumfalciparuminmosquitoesJ!.TransRSocTropMedHyg,1993,87(3:273275.17!GreenCA,RattanarithikulR,PongparitS,etal.Anewlyrecognizedvectorofhumanmalarialparasitesintheorientalregion,Anopheles(cellia)pseudowillmori(theobald,1910)J!.TransRSocTropMedHyg,1991,85(1):3536.18!GreenCA,RattanarithikulR,CharoensubA.PopulationgeneticconfirmationofspeciesstatusofthemalariavectorsAnopheleswillmoriandAnophelespseudowillmoriinThailandandchromosomephylogenyofthemaculatusgroupofmosquitoesJ!.MedVetEntomol,1992,6(4):335341.19!,,,.J!.,1996,3(2):100105.:20100104;:20100303653
本文标题:中国致病性小肠结肠炎耶尔森菌生物分型研究
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