四种不同品种水蛭生物活性的研究与比较(PDFfile)-

[],,(,201203):2005204217:(014319318):(1966),(),,,,:021250800300,E2mail:[e-mail]:;;;::3,:3,:4,;4:3:R284.1:A:100121528(2006)0220232203BiologicalactivitiesoffourkindsofleechesWUZhi2jun,ZHANGLing2xia,YULi2hua(ShanghaiCenterofResearchandDevelopmentofNewDrugs,Shanghai201203,China)KEYWORDS:leeches;antithrombin;antitrypsin;antichymotrypsinABSTRACT:AIM:Toprovidescientificreferencesforleechesbymeansoftheantithrombin,antitrypsinandan2tichymotrypsinactivitiesofthefourkindsofleeches.METHODS:Usingantithrombin,antitrypsinandantichymot2rypsinactivityanalysismethods,thefourkindsofleeches,theywere,WhitmaniapigraWhitman,HirudonipponiaWhitman,HirudomedicinalisLinnaeus,HirudinariamanillensisLesson,weredetected.RESULTS:Theblood2suckingleechesexceptwhitnaniapigrawhitmanhadhighantithrombinactivity,andthesefourkindsofleecheswerethesameantitrypsinandantichymotrypsinactivities.CONCLUSION:Thesethreebiologicalactivitymethodscouldbeconsideredasreliableindexesinassessingthequalitityofleeches.,19,1963(WhitmaniapigraWhitman,)(WhitmaniaacranulateWhitman,)(HirudonipponicaWhitman,)2005,,[1],[26],,[7]34,11.1,;,;(HirudinariamanillensisLesson),23220062282ChineseTraditionalPatentMedicineFebruary2006Vol.28No.2(HirudomedicinalisLinnaeus),,Baskova1.2K.A21000()(Sartorius)(EBPA)pH/CondLevel3(WTW)YJ501()(LABCONCO)J22HS(BECKMAN)HH2S()MDF2382E(SANYO)1.3(Sigma);(Sigma):N22L2(BAEE,);();N22L2(ATEE,Sigma);()22.1,,,,30min,-80,415,-8030min,,-30,24,2.20.1g3,,2.5mL0.9NaCl,,2530min,(3500r/min,8min)3,2.32.3.1,[8]1cm,7.5cm0.5(50mmol/LTris2HCl,pH7.4)200L10L,,370.5,(100.0,50.0,20.0,10.0NIH/mL,)5L,,,1min,11,(NIH)(ATU)2.3.2,2005[9]2.3.3,[10]2005[9]334,114(u/g)n=5(u/g)n=3(u/g)n=35021.8325.05150012.1537.53140011.2523.61135021.9029.794,,;[17],:,,;,G(1),,,;,;Piyavit34,333220062282ChineseTraditionalPatentMedicineFebruary2006Vol.28No.2,,,,:[1]BaskovaIP,KhalilS,NikonovGI,etal.EffectofthesecretionofSalivaryGlandsinthemedicinalleechHirudinomedicinalisontheexternalandinternalbloodcoagulationmechanisms[J].ByullEkspBiolMed,1984,97:142.[2]FritzH,GebhardtM,MeisterR,etal.Trypsin2plasmininhibi2torsfromLeeches:Isolation,aminoacidcomposition,inhibitorycharacteristics[C].ProcIntRes,1971,2712280.[3]BaskovaIP,ZavalovaLL.Proteinaseinhibitorsfromthemedic2inalleechHirudomedicinalis[J].Biochem(Moscow),2001,66(7):7032714.[4]BaskovaIP,NikonovGI,CherkesovaOU,etal.Antithrom2bin,antitrypsinandantichymotrypsinactivitiesofthesalivaryglandsecretionandintestinalchymeofmedicinalleeches[J].Fo2liaHaematol,Leipzig,1984,111(6):8312837.[5]FradkovA,BerezhnoyS,BarsovaE,etal.Enzymefromtheme2dicinalleech(Hirudomedicinalis)thatspecificallysplitsendo2(22Glu)2Lysisopeptidebonds:cDNAcloningandproteinprima2rystructure[J].PEBSletters,1996,390:1452148.[6]NikonovGI,BaskovaIP.Antithromboticeffectoftheprepara2tionsfromtheleechesHirudomedicinalis[J].FoliaHaematol,1988,115(122):47251.[7],,,.[J].,1998,7(2):91.[8][S].2005:57.[9][S].2005:241.[10]BeatriceK.NaturallyoccurringinhibitorsofproteolyticEnzymes[C].MethodsEnzymol,1970,19:8392885.,,(,230061):2004210219:(1954),,,,:055122821836:;;;;;;:::,DB2INNOWax,,FID,:,890mg/L,2170mg/L,2000mg/L;,96.8104.4,0.1252.87mg/L:,,,:R284.1:A:100121528(2006)0220234202DeterminationofresiduumfromgardeniaextractbymacroporousadsorptionresinZHONGHuai2bin,SUNLin2yun,TONGCheng2liang(AnhuiProvincialInsititualforDrugControl,Hefei230061,China)KEYWORDS:extractfromgardenia;residuum;capillarycolumn;methylbenzene;dimethylbenzene;divinyl2benzene;phenyletheneABSTRACT:AIM:Toestablishthemethodofdeterminingmethylbenzenedimethylbenzenedivinylbenzeneandphenyletheneingardeniaextract.METHODS:TheGCsystemconsistedofDB2INNOWaxcapillarychromato2graphiccolumn,nitrogenasthecarriergas,andFIDasthedetector.RESULTS:Methylbenzenewaslessthan43220062282ChineseTraditionalPatentMedicineFebruary2006Vol.28No.2