牛ANGPTL4基因结构和功能的生物信息学分析

20103210135－0140http//xuebao.jx[e-mail]4121111331.4640002.4633003.712100ANGPTL4、、、、、。ANGPTL4。12α－8β－303。ANGPTL4、、、、、、。－4ANGPTL4S823.2A1000－2286201001－0135－06BioinformaticsAnalysisofBovineANGPTL4GeneStructureandFunctionMAYun1LUOYing-ying2LIFen1WANGJun-fang1XUYuan1YAOJin1LIRong-rong3CHENHong31.CollegeofLifeScienceXinyangNormalUniversityXinyang464000China2.TheFirstSeniorMid-dleSchoolofPingyuCountyZhumadian463300China3.CollegeofAnimalScienceandTechnologyNorth-westAgricultural＆ForestryUniversityYangling712100ChinaAbstractANGPTL4geneincattlewasinvestigated.Bymeansofbioinformaticsanalysisitsencodingpro-teinwasanalyzedincludingitsstructurephysico－chemicalpropertysignalpeptidetransmembranestruc-turesubcellularlocalizationsecondarystructureandhigherstructureanditsevolutionrelationshipwithotherspecieswasinferred.TheresultsindicatedthatANGPTL4proteinofcattlewasakindofhydrophobicitylabileproteinlocatingcellularexteriorwhichcontained12α－helics8β－strands30turnsand3transmem-branedomains.ItmightbeconcludedthatANGPTL4geneofcattlehascloseetaffinitywiththatofpigcom-paredwiththatofhumanmusratpandogandfowlbasedontheanalysisofmolecularevolution.Keywordscattleangiopoietin－like4ANGPTL4sequenceanalysisbioinformatics4ANGPTL41999fastinginducedadiposefactorFIAF1。YoonANGPTL4αPPARαγPPARγperoxisomeprolife-rati－receptorPPAR－gammaangiopoietin－relatedPGAR2。2002HUGO2009－09－182009－11－24092300410010、[e-mail]。32ANGPTL4angiopoietin－like43。4ANGPT4－56。LeGbaguidiANGPTL4PPARγANGPTL47－8。PPARANGPTL49－10。ANGPTL4。11ANGPTL4CDSDNAPCR－SSCPDNAANGPTL48ANGPTL4。ANGPTL4。ANGPTL4。11.1NCBIhttp//－form.html－model.htmlDNAStar。1.2NCBIANGPTL4cDNADNAStarEditseqhttp//－param.htmlANGPTL4DNAStarMegalignDNAStarProteanChou－FasmanANGPTL4http//－form.htmlANGPTL4WOLFPsortANGPTL4http//swissmodel.ex-pasy.org/ANGPTL4。22.1ANGPTL4ANGPTL4410DNAStarEditseqANGPTL445552.47Da47KR11.46%40DE9.76%128AILFWV31.22%106NCQSTY25.85%8.758。A=19.71%243G=31.39%387T=15.65%193C=33.25%410Ambiguous=0.00%0A+T=35.36%436C+G=64.64%797。ANGPTL44。CGAT。2.2ANGPTL4ANGPTL4410。http//。ANGPTL4C1997H3126N610O589S14。280nm6295053.1270.54－0.6491。20Leu、·631·1ANGPTL41ANGPTL4ANGPTL4Fig.1PhylogenetictreeoftheaminoacidsequencesofcattleANGPTL4andotherANGPTL4proteinsinhumanandotheranimals2ANGPTL4Fig.2SecondarystructureofANGPTL4proteinsGly、Gln、AlaTyr。2.3ANGPTL4、、、、、、ANGPTL4ANGPTL4DNAS-tarMegalign。17。、、、。。ANGPTL4ANGPTL4。2.4ANGPTL4、。12－14。2ProteanANGPTL4。2Chou－FasmanANGPTL412ɑ6～2533～8193～97109～204261～289376～3808β209～212232～236294～324368～374385～391396～4043029～3262～158192～220229～260275～302317～366381～395405～408。2.5ANGPTL4。。。http//。·731·323ANGPTL4Fig.3AnalyzedsignalpeptideofANGPTL4proteins4ANGPTL4Fig.4AnalyzedtransmembranedomainsofANGPTL4proteins3CSY。C、Y、SYES。CS。23～24。2.6ANGPTL420“”。、、。Tampered－form.htm14。3～25318～336351～371333。2.7ANGPTL4、、。WOLFPsort·831·1ANGPTL45ANGPTL4Fig.5Three－dimensionalstructureofANGPTL4proteins15、、、、。93.1%。ANGPTL4KusedforkNNis32queryProteindetailsextr14.0pero7.0mito4.0E.R3.0lyso2.0。KK－NearestNeighborskNNKeller161985。。K。KK。。2.8ANGPTL4。http//swissmodel.expasy.org/ANGPTL45。3、、ANGPTL4。ANGPTL4ANGPTL4。ANGPTL417。ANGPTL4N18－19。Antonio20。。ANGPTL4ANGPTL4。LeJan7ANGPTL4CHOCHO/ANGPTL4。ANGPTL4Yoshida21ANGPTL4。ANGPTL4ANGPTL4LPL。ANGPTL410－22。ANGPTL4。Xu10ANGPTL4、、、。23ANGPTL4ANGPTL5ANGPTL42RT－PCRANGPTL4Xu10HepG2、ANGPTL4ANGPTL4mRNAANGPTL4。ANGPTL4。·931·32ANGPTL4ANGPTL4ANGPTL4ANGPTL4、。1KerstenSMandardSTanNSetal.Characterizationofthefasting－inducedadiposefactorFIAFAnovelperoxisomeprolif-er－ator－activatedreceptortargetgeneJ.JBiolChem20002753728488－28493.2YoonJCChickeringTWRosenEDetal.Peroxisomeprolifer－ator－activatedreceptorgammatargetgeneencodingano-velangiopoietin－relatedproteinassociatedwithadiposedifferentiationJ.MolCellBiol200020145343－5349.3WanDFGongYWenXQetal.Large－scalecDNAtrans－fectionscreeningforgenesrelatedtocancerdevelopmentandprogressionJ.ProcNatlAcadSciUSA20041014415724－15729.4SukoninaVLookeneAOlivercronaTetal.Angiopoietin－likeprotein4convertslipoproteinlipasetoinactivemonomersandmodulateslipaseactivityinadiposetissueJ.ProcNatlAcadSciUSA20061034617450－17455.5LichtensteinLBerbeeJFVanDijkSJetal.ANGPTL4upregulatescholesterolsynthesisinliverviainhibitionofLPLandHL－dependenthepaticcholesteroluptakeJ.ArteriosclerThrombVascBiol200727112420－2427.6ZhuHLiJQinWetal.CloningofanovelgeneANGPTL4andthefunctionalstudyinangiogenesisJ.ZhonghuaYiXueZaZhi200282294－99.7LeJanSAmyCCazesAetal.Angiopoietin－like4isaproangiogenicfactorproducedduringischemiaandinconvention-alrenalcellcarcinomaJ.AmJPathol200316251521－1528.8GbaguidiFGChinettiGMilosavljevicDetal.Peroxisomeproliferator－activatedreceptorPP