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,(,455000)PCV,PCV-1PCV-2,PCV,PCV-1RepPCV,PCV-2PCV-2,;PCV;S854A0517-6611(2007)13-03870-03ResearchProgessofMoleculeBiologyofPorcineCircovirusHAOGui-zengetal(DepartmentofAnimalHusbandryandVeterinary,AnyangCollegeofTechnologyofHenanProvince,Anyang,Henan,455000)AbstractThecharacteristicsofmoleculebiologyofPCVwereintroduced.ItwasconsideredthattherewasarelatedrelationshipbetweenPCV-1andPCV-2,buttherewasalsoagreatervariationbetweenthem.PCVgenomewascopiedinmodeofrollring.ThecopyofPCV-1genomewasdependedonRepprotein.ThecharacteristicsofmoleculeimmunologyofPCVweredepicted.ItwasconsideredthatPCV-2couldharmtheimmunitysystemofswinebodyandinduceimmunityrestraintafteritinfectedtheswine.BasedonmoleculeepidemiologyitwasconcludedthatthegenomeofdifferentseparatedstrainsofPCV-2wasmorestablecommonly,buttheseparatedstrainsfromdifferentregionhadsomechangesongenomesequence.Thenewproblemsonresearchofcircoviruswerebroughtforward.KeywordsSwine;PCV;Circovirus(PorcineCircovius,PCV),PCVDNA,20,1720nm,CsCl1.37,52S;pH3,5670;PCV,PCVPCV1PCV22PCV1,PCV-PK-15,;PCV2,PCV2PCV2(PostweaningMultisystemicWastingSyn-drome,PMWS),PCV2(PorcineDer-matitisandNephropathySyndrome,PDNS),(CongenitalTremors,CT)(ProliferativeandNecrotizingPneumonia,PNP),11.1PCVPCV11759bp,PCV217671768bpPCV5-TAGTATTAC-3-(Stem-loopStructure)-PCV2PCV180%[1-2],1900bp82%,90117681759bp62%(2003033120)(1964-),,,,,2007-01-2976%PCV1PCV22(ORFs)ORF1ORF2ORF183%,86%;ORF267%,65%[3],GenBankPCV295%,PCV199%,PCV1PCV2,PCV175kDa(ORFs),,2ORF1ORF2RepRepCapPCV14polyA,314319bp973978bp10151030bp11841189bpPCV211236kDaORFs,ORF1ORF11,(1),ORF1ORF5ORF7ORF105-3;ORF2ORF3ORF4ORF6ORF8ORF9ORF115-3,,ORF1945,315,RepORF1;ORF2705,234,PCV2,30kDa,ORF13,ORF2ORF3111,ORF1ORF2ORF2PCV2LiuPCV2ORF2,ORF2N41PCV2[4],12183441ORF24,6983117131PCV2,PCV23polyA,327332bp983988bp10221027bp,JournalofAnhuiAgri.Sci.2007,35(13):3870-38721PCV-2bp1945327051331514210059006900760086609129010108011450NawagitgulORF2,ORF2[5]MaheORF1ORF2,PCV1ORF2PCV2ORF2,ORF2PCV-2[6]LiuORF2MBP-His(8)-tag3,ORF2,PCV2[7]ORF2PCV1PCV2TruongORF2PCV[8]ORF2,PCV1.2PCV,(ds)(rf)DNA22PCV2PSK,PK15,,PCVDNAPCV1RepRep757777bp,36.5kDaRep(11761558bp),,,Rep,19.2kDaRep,Rep,PCV1,PRep(640796bp),PCV1;PRep(13281252bp),RepRepP,111bp(728838bp)DNA(5-TAGTAT-TAC-3)CG-GCAGCGG/TCAG2,Rep(ElectophereticMo-bilityShiftAssay,EMSA)PCV112RNA,1RNA8RNAs(RepRepRep3aRep3bRep3c-1Rep3c-2Rep3c-3Rep3c-4)3NSRNA(NS462NS642NS0)8RepRNA53,3NSRNA3Rep7RepRNA,3NSRNAsORF1Rep3PCV2PK159RNAs,1RNA5RNAs(RepRepRep3aRep3bRep3c)3NSRNAs(NS515NS672NS0),PCV1PCV2RepRepRep3aRep3bNS0,Rep3aNSRNA22.1PCV2PCV2,()Otali,PCV2Peyer,PCV2TB,PCV2[9]MagarSPF,PCV2[10]Steven-son4PCV,PCV2[11]Rossel30PDNSPCV2,PCV2[12]SanchezPCV2,PCV2,[13]2.2BTShibaharaPMWS,PCVB,[14]Segales,PCV2,PMWS,;,BT(CD4+),PMWS,[15]DarwichCD4+CD8+CD4+CD8+()IgM+,PCV2CD8+CD4+CD8+0.05;,BTCD8+[16],PMWS3,,(Interdigitat-ingCells),BCD4+T;,PCV2BCD4+T[24]Chiani-ni,PMWSBT[17],PCV2,2.3PMWSDarwichRT-PCRPMWS15817833513,,,mRNA,IL-10mRNA[18],T,,PCV2IL-8,IFN-,PCV2TH1;,PCV2IL-10,IL-10,PCV2,PCV2McNeilly,PCV2,Fc,4dPCV2MHCI,8dMHCII[19]2.4PMWSPMWSKrakowka,PMWS[20]Kim-1(MCP-1)-8(IL-8)DNA(ISH),MCP-1PMWS[21]3Fenaux6PCV2,PCV2[22],2PCV2PCV2,,,PCV2,PCV2Larochelle1990200134PCV236PCV2[23],34PCV2,PCV2,ORF2ORF1,3,270PCV2,,4PMWS,PCV2,PCV95%,5%15%,40%,PCV2,PCV2,,PCV2PCV2[1]MEEHANB,MCNEILLYF,TODDD,etal.Characterizationofnovelcir-covirusDNAsassociatedwithwastingsyndromesinpigs[J].JGenVirol,1997,79:2171-2179.[2]ALLANGM,MEEHANB,TODDD,etal.Novelporcinecircovirusfrompigswithwastingdiseasesyndromes[J].VetRec,1998,142:467-468.[3]SEGALES,DOMJNGOM.Postweaningmultisystemicwastingsyndrome(PMWS)inpigs[J].AReview,VeterinaryQuarterly,2002,24(3):109-124.[4]LIUQ,TIKOOSK,BABIUKLA.NuclearlocatizationoftheORF2proteinen-codedbyporcinecircovirustype2[J].Virology,2001,285(1):91-99.[5]NAWAGITGULP,MOROZOVI,BOLINSR,etal.Openreadingframe2ofporcinecircovirustype2encodesamajorcapsidprotein[J].JGenVirol,2000,81(9):2281-2287.[6]MAHED,BLANCHARDP,TRUONGC,etal.DifferentialrecognitionofORF2proteinfromtype1andtype2porcinecircovirusandidentificationofim-munorelevantepitopes[J].JGenVirol,2000,81(7):1818-1824.[7]LIUQ,WILLSONP,ATTOPOKUS.BacterialexpressionofanimmunologicallyreactivePCV-2ORF2fussionproteion[J].ProteionExprPurif,2001,21(1):115-120.[8]TRUONGC,MATHED,BLANCHARDP,etal.Identificationofanimmunorele-vantORF2epitopefromporcinecircovirustype2asaserogicalmarkerforex-perimentalandnaturalinfection[J].ArchVirol,2001,146(6):1197-1121.[9]OTALIY,NUNOYAT.DepressionofvaccinalimmunitytoMarkesdiseasebyinfectionwithanemiaagent[J].AvianPathology,1998,17:333-347.[10]MAGARR,LAROCHELLER,THIBAULTS,etal.Exprementaltransmissionofporcinecircovirustype2(PCV2)inweanedpigs:asequentialstudy[J].JournalofComparativePathology,2000,123(4):258-269.[11]STEVENSONGW,KIUPELM,MITTALSK,etal.Tissuedistributionandge-neticcongentialtremors[J].JournalofVeterinaryDiagnosticInvestigation,2001,13(1):57-62.[12]ROSELLC,SEGALESJ,RAMOSVARAJA,etal.Identificationofporcinecir-covirusintissuesofpigswithprocingdermatitisandnephropathysyndrome[J].VetRec,2000,146(2):40-43.[13]SANCHEZRJ,MEERTSP,NAUWY
本文标题:猪圆环病毒的分子生物学研究进展
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