生物强化技术及其在废水生物处理中的应用

19Vol.1,No.920079ChineseJournalofEnvironmentalEngineeringSep.200711323(1.,150090;2.,523581;3.,518055),,,,X703.1A167329108(2007)0920040206BioaugmentationtechnologyanditsapplicationinbiologicalwastewatertreatmentWangJianfang1ZhaoQingliang1LinJikan2JinWenbiao3(11SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090;2.GoldenBio2TreatTechnologyLimited,Dongguan523581;3.HarbinInstituteofTechnologyCampusofShenzhenUniversityTown,Shenzhen518055)AbstractBioaugmentationtechnologyhasbeenwidelyusedinbiologicalwastewatertreatmentinrecentyears.Thescreenofhighefficientbacteriawithstrongeradaptabilityandcompetitioninnewenvironmentisoneofdecisivefactorsforbioaugmentationtechnology.Thepaperelaboratesthreemethodsforaugmentationofcatabolicmobilegeneticelements,geneengineeringstrainsandbacteriawhichwereisolatedbyconventionallymicrobiolog2icaltools.Theapplicationsofbioaugmentationtechnologyinbiologicalwastewatertreatmentforremovalofrecal2citrantcontaminantsandredundantnutrientssuchasN,P,andmaintainingstabilityofecologicalsystemsaredis2cussed.Keywordsscreenofeffectiveandefficientbacteria;geneengineering;mobilegeneticelements;biologi2calwastewatertreatment:2006-03-28;:2007-07-20:(1973),,,E2mail:[e-mail]3,E2mail:[e-mail],,CO2H2O,,,,,,,,,,,[1],,©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:(1);(2);(3),1.1(catabolicmobilegeneticelements),,[2][3,4],,Springael[5]2P.putidaBN210,32(3CBA)clcPCR2DGGE,2BN210,3CBA,23,clc,Mancini[6],,,;,,,,,1.2,,Dai[7]Sphingobilumchlorophenolicum10,3mmol/L,Watanabe[8],C.testosteroniR5phc,Co2mamonassp.rN7,rN7,R5335d,PCR,phc,108,phcR5,,,,,,,,:(1);(2);(3),,1.31.3.1,,,14©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:;,,,,,,Simon[9]2,,2Oerther[10]SBRAcinetobactersp.,,,,,P.aeruginosa,[11],1.3.3,,,,,;,vanderGast[1214]:;();,,85%,,1.3.4,Bouchez[15]SBR,,,,,,,,pHDO,,,,,,,,[16,17]22.124©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[18],22(A/A/O)Burkholderiapickettii,COD25%16%59%Shi[19]2,42DCP,,2,42DCP2,42DCP,422,4,52,[20,21][22][17][23],,,,,,,,2.2,,,,,[24],Satoh[25],,,Head[26],Belia[27]Acinetobacterlwoffii,,(EBPR),14dEBPR,2.3(pH)[28],,,Boon[29]32,,322d,,12d,COD36%;4d,COD,3232,,3,,,,,,,,,34©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(FISH)[29,30](DGGE/TGGE)[31],[26],,,,,,,[1]AlvesC.F.,MeloL.F.,VieiraM.J.Influenceofmedi2umcompositiononthecharacteristicsofadenitrifyingbio2filmformedbyAlcaligenesdenitrificansinafluidizedbedreactor.ProcessBiochem.,2002,37(8):837845[2]TopE.M.,SpringaelD.,BoonN.Catabolicmobilege2neticelementsandtheirpotentialuseinbioaugmentationofpollutedsoilsandwaters.FEMSMicrobiol.Ecol.,2002,42:199208[3]vanderMeerJ.R.,RavatnR.,SentchiloV.Theclcele2mentofPseudomonassp.strainB13andothermobiledeg2radativeelementsemployingphage2likeintegrases.Arch.Microbiol.,2001,175:7985[4]TsudaM.,TanH.M.,NishiA.,etal.Mobilecatabolicgenesinbacteria.J.Biosci.Bioeng.,1999,87:401410[5]SpringaelD.,PeysK.,RyngaertA.,etal.Communityshiftsinseeded32chlorobenzoatedegradingmembranebio2filmreactor:Indicationsforinvolvementofinsituhorizon2taltransferoftheclc2elementfrominoculumtocontaminantbacteria.Environ.Microbiol.,2002,4:7080[6]ManciniP.,FertelsS.,NaveD.,etal.MobilizationofplasmidpHSV106fromEscherichiacoliHB101onalabora2tory2scalewastetreatmentfacility.Appl.Environ.Micro2biol.,1987,53:665671[7]DaiM.,CopleyS.D.Genomeshufflingimprovesdegrada2tionoftheanthropogenicpesticidepentachlorophenolbySphingobiumchlorophenolicumATCC39723.Appl.Envi2ron.Microbiol.,2004,70:23912397[8]WatanabeK.,TeramotoM.,HarayamaS.Stablebioaug2mentationofactivatedsludgewithforeigncatabolicgenesharbouredbyanindigenousdominantbacterium.Environ.Mictobiol.,2002,4:577583[9]SimonM.A.,BonnerJ.S.,PageC.A.,etal.Evalua2tionoftwocommercialbioaugmentationproductsforen2hancedremovalofpetroleumfromawetland.Ecol.Eng.,2004,22:263277[10]OertherD.B.,DanalewichJ.,DulekgurgenE.,etal.Bioaugmentationofsequencingbatchreactorsforbiologi2calphosphorusremoval:ComparativerRNAsequencea2nalysisandhybridizationwitholigonucleotideprobes.Wat.Sci.Tech.,1998,37(45):469476[11]SingerA.C.,vanderGastC.J.,ThompsonI.P.Per2spectivesandvisionforstrainselectioninbioaugmenta2tion.TrendsinBiotechnol.,2005,23(2):7477[12]vanderGastC.J.,WhiteleyA.S.,StarkeyM.,etal.Bioaugmentationstrategiesforremediatingmixedchemicaleffluents.Biotechnol.Progr.,2003(a),19:11561161[13]vanderGastC.J.,WhiteleyA.S.,LilleyA.K.,etal.Bacterialcommunitystructureandfunctioninametal2workingfluid.Environ.Microbiol.,2003(b),5:453461[14]vanderGastC.J.,WhiteleyA.S.,ThompsonI.P.Temporaldynamicsanddegradationactivityofanbacterialinoculumfortreatingwastemetal2workingfluid.Environ.Microbiol.,2004,6(3):254263[15]BouchezT.,PatureauD.,DabertP.,etal.Ecologicalstudyofabioaugmentationfailure.Environ.Microbiol.,2000,2:179190[16