生物法合成5-氨基乙酰丙酸的研究进展

5-,,(,310027):5-(ALA),ALA,ALA,ALA,ALA:5-;;;:TQ033:A:0253-4320(2008)S2-0190-04Recentadvancesinbiosynthesisof52aminolevulinicacidFUWei2qi,LINJian2ping,CENPei2lin(DepartmentofChemicalandBiochemicalEngineering,ZhejiangUniversity,Hangzhou310027,China)Abstract:Theresearchprogressinthebiosynthesisof52aminolevulinicacid(ALA)athomeandabroadisreviewed.AndtheapplicationofinducedmutationbreedingmethodandmetabolicengineeringtechnologyinALAbiotechnologicalproductionwasdiscussedindetail.FurtherstudyonALAproductioncanbeexpectedtoleadtohighproductivityofALAviaregulationofmetabolicpathwayinmircoorganisms.Keywords:52aminolevulinicacid;biosynthesis;inducedmutationbreeding;metabolicengineering:2008-09-10(366)3(B5)(),,;(),,,,,,j@zj5-(ALA)B12,[1]1999,ALA(FoodandDrugAdministration,USA),ALA[2-4],ALA,,[5-6],ALA[7-8],[9]ALA,ALA,,[10],,ALAALA,1ALAALA2[1]:(C4pathway)(C5pathway),ALAAALA,ALA31ALA091Oct.200828(2)ModernChemicalIndustry200810:20002972007C70780:1982-1971-linpu.edu.cn,tRNA1ALAALAALA,ALA,ALA,ALA,,ALA,,ALA,ALA,ALA2ALAALA2:,ALA,,ALA;,ALA,ALA,,ALA211ALA,,,Andersen[11]1983ALAALA,,1987,Sasaki[12]ALA(hemB)(LA),,ALA0126g/L(2mmol/L),ALA,NishikawaKamiyama(R1sphaeroides)[3],LR66,L16L(L)N[15](R1sphaeroides),ALACR-0072009,,017616L/L,ALA27nmol/(minmg);,74hALA712g/L(55mmol/L),ALA,20,NishikawaALACR-0072009,0126g/L712g/L,,ALA212ALADNA,,[16],,,ALAALA,ALA,ALA,ALAC4ALA,ALA(ALAS);C5,C4ALAS(hemA),ALASALA,ALA1996,MarietZeikus[17](R1sphaeroides)ALAhemApUCl8/19E1coliDH1,ALA0129g/L1999,Choi[18]E1coliBL21(DE3),(Brandyrhizobiumj),,1(3L),L16L3,191200810:5-1-14AAC-0AA2g/20mmol/ishikawaaponicumhemApH700mmol/AA2g/200Lee[19]/ALA,318g/L,Xie[20](R1sphaeroides)hemAE1coliMG1655,,ALA512g/L2004,Kang[21]Rhodobactercapsula2tushemA,E1coliBL21(DE3),218g/LALA2005Chung[22]B1japonicumALA,ALA,ALA113g/L2008,Choi[23]Rhodopseu2domonaspalustrisKUGB306hemA,pGEX-KGE1coliBL21(DE3),ALA,ALA511g/L2005,Liu[24]Agrobacteriumradiobacterzju0121ALAE1coliBL21(DE3),019g/LALA;,ALA3101g/L[25]2007,[26](Rhodoblastusacidophilus)hemAM15,514g/L2007,Fu[27](R1sphaeroides)hemARosseta(DE3),,28hALA616g/L(50mmol/L)[28]ALA,ALA15L712g/L(55mmol/L)20901996,10,ALA,ALA0129g/L712g/L(55mmol/L),,,ALA3L,L105,,,ALA,,,,ALA,,[1]VladimirYB,DemainAL,ZaitsevaNI.ThecrucialcontributionofstarvedrestingcellstotheelucidationofthepathwayofvitaminB12biosynthesis[J].CriticalReviewsinBiotechnology,1997,17(1):21-37.[2]KennedyJC,PottierRH,ProssDC.Photodynamictherapywithen2dogenousprotoporphyrinL:IX.Basicprincipalsandpresentclinicalex2perience[J].JournalofPhotochemistryandPhotobiologyB2Biology,1990,6(1/2):143-148.[3]DeDominicisC,LibertiM,PerugiaG,etal.Roleof52aminolevulinicacidinthediagnosisandtreatmentofsuperficialbladdercancer:Im2provementindiagnosissensitivity[J].Urology,2001,57(6):1059-1062.[4]ChenHM,ChenCH,YangH,etal.Successfultreatmentoforalverru2coushyperplasiawithtopical52aminolevulinicacid2mediatedphotody2namictherapy[J].OralOncology,2004,40(6):630-637.[5]SasikalaCH,RamanaV,RaghuveerRP.52Aminolevulinicacid:Apo2tentialherbicide/insecticidefrommicroorganism[J].BiotechnologyProgress,1994,10(5):451-459.[6]SasakiK,WatanabeM,TanakaT,etal.Biosynthesis,biotechnologicalproductionandapplicationsof52aminolevulinicacid[J].AppliedMi2crobiologyandBiotechnology,2002,58(1):23-29.[7]HottaY,WatanabeK.Plantgrowth2regulatingactivitiesof52aminole2vulinicacid[J].Syokubutu2no2Kagaku2Tyousetu(Chemicalregulationofplants)1999,34(1):85-96.[8]KuramochiH,KonnaiM,TanakaT,etal.Methodforimprovingplantsalttolerance:US,5661111[P].1997-08-26.[9]TanakaT,TakahashiK,HottaY,etal.Methodforpromotingplantgrowthusing52aminolevulinicacidorasaltthereof:US,5298482[P].1994-03-29.[10]MiyachiN,TanakaT,NishikawaS,etal.Preparationandchemicalpropertiesof52aminolevulinicacidanditsderivatives[J].Porphyrins,1998,7(2/3):342-347.[]T,BT,NT,Mfyf52,2[]FMSMyL,3,(3)3336[]SK,IS,NzY,f5229128(2)AAAA11Andersenriseidesbakkenetal.echanismsosnthesisoaminolevulinateinpurplegreenandbluegreenbacteriaJ.Eicrobiologetters198192/:0-0.12asakikedaishiawaetal.Productionoaminolevulinicacidbyphotosyntheticbacteria[J].JournalofFermentationandBio2engineering,1987,65(5):511-515.[13]NishikawaS,WatanabeK,TanakaT,etal.Rhodobactersphaeroidesmutantswhichaccumulate52aminolevulinicacidunderaerobicanddarkconditions[J].JournalofBioscienceandBioengineering,1999,87(6):798-804.[14]KamiyamaH,HottaY,TanakaT,etal.Productionof52aminolevulinicacidbyamutantstrainofaphotosyntheticbacteria[J].Seibutsu2Ko2gakuKaishi,2000,78(2):48-55.[15]NishikawaS,TanakaT,KaminagaT,etal.Microorganismsproducing52aminolevulinicacidandprocessingforproducing52aminolevulinicacidbyusingthesame:US,6342377[P].2002-01-29.[16]BaileyJE.Towardsascienceofmetabolicengineering[J].Science,1991,252(5013):1668-1674.[17]MarietJ,WerfVD,ZeikusJG.52aminolevulinateproductionbyEs2cherichiacolicontainingtheRhodobactersphaeroideshemAgene[J].AppliedandEnvironmentalMicrobiology,1996,62(10):3560-3566.[18]ChoiC,HongB,SungH,etal.Optimizationofextracellular52aminole2vulinicacidproductionfromEscherichiacolitransformedwithALAsyn2thasegeneofBradyrhizobiumjaponicum[J].Biotech