斑马鱼的实验室养殖方法及其实验应用

294201012JournalofWuhanPolytechnicUniversityVol29No4Dec2010:20100603.:(1986),,,Emai:[e-mail].:(1965),,,Emai:[e-mail].:10094881(2010)04001304DOI:10.3969/.jissn.10094881.2010.04.004斑马鱼的实验室养殖方法及其实验应用1,2(1.,430023;2.,430023):斑马鱼作为一种新兴的模式动物,近年来其在分子发育生物学环境毒理学等学科研究上的应用日益广泛结合作者在实验室养殖斑马鱼的经验,综述了斑马鱼的特性日常管理及其在以上研究领域的应用:斑马鱼;实验室;养殖;应用:Q95331:AZebrafishlaboratoryculturetechniquesZHOUYin1,ZHANGHongling2(1.DepartmentofBiologicalandPharmaceuticalEngineering,WuhanPolytechnicUniversity,Wuhan430023,China;2.DepartmentofHealthScienceandNursing,WuhanPolytechnicUniversity,Wuhan430023,China)Abstrac:tAsannewmodelanima,ltheapplicationofzebrafishinmoleculardevelopmentalbiology,environmentaltoxicologyandotherscientificresearchisincreasinglywidespreadinrecentyears.Thisarticlereviewstheapplicationofzebrafishinresearcharearecentyearscombinedwiththeauthorszebrafishcultureexperienceinthelaboratory.Keywords:zebrafish;laboratory;culture;application(Daniorerio,zebrafish),,,,,[1],(11.5mm),,,,,[2],,,[3]11.1,46cm,,,2010,;,,,,1.22430[4],,11,,,2,pH78,1d,1/3,,,,[5],,23,,5min,,,,,,,,,,,,,84,,33.11012,1718,1!21!114h10h,34h,1mm,,,,,3.2,,()1,,(1):,()144,:,,,,,,3.3,2528,,1∀,,56h,3.43672h,23d,,,,,,,,44.1,(3d),,1996,[6],,Herbomel[7]WholeMountLplastin,KorfsmeierCole[89]4.2,,[10],,2001Yoder[11](leukocytereceptorcluster),Weinstein[12]gridlock,,,[13]Martina[14],(EDCs),4.3:(35d);,;,,;,,,7d;,;,,,Phylonix152010,1994,ZFIN(),,,,,,2006,#∃(),,20,,:[1]程烽,陈竺.斑马鱼:一种造血系统的遗传学研究模型动物[J].国外医学输血及血液学分册,2004,27(3):200202.[2]吴玉萍,熊茜,张广献,等.斑马鱼发育遗传学研究进展[J].遗传学报,2004,31(10):11671174.[3]王跃祥,钟涛,宋后燕.斑马鱼基因工程的研究进展[J].国外医学遗传学分册,2004,27(4):220223.[4]MatthewsM,TrevarrowB.Avirtualtouroftheguideforzebrafishusers[J].LabAnim(NY),2002,31(3):3440.[5]HelfmanG,ColletteB,FaceyD.TheDiversityofFishes[M].MA,BlackwellPublishingCompany,1997:528.[6]HafferP,GranatpM,BrandM,etal.Theidentificationofgeneswithuniqueandessentialfuctionsinthedevelopmentofthezebrafish,Daniorerio[J].Developmen,t1996,123:136.[7]HerbomelP,ThisseBandThisseC.Ontogenyandbehaviourofearlymacrophagesinthezebrafishembryo[J].Developmen,t1999,126(17):37353745.[8]KorfsmeierKH.PCNAintheovaryofzebrafish(Brachydaniorerio)[J].ActaHistochem,2002,104:7376.[9]ColeLK,RossLS.Apoptosisinthedevelopingzebrafishembryo[J].DevBio,l2001,240:123142.[10]CorleySmithGE,BrandhorstBP,WalkerC,etal.Productionofhaploidanddiploidandrogeneticzebrafish(includingmethodologyfordelayedinvitrofertilization)[J].MethodsCellBio,l1999,59:4560.[11]YoderJA,MuellerMG,WeinsteinBM,etal.Immunetypereceptorgenesinzebrafishsharegeneticandfunctionalpropertieswithgenesencodedbythemammalianleukocytereceptorcluster[J].ProcNatlAcadSciUSA,2001,98:67716776.[12]WeinsteinBM,StempleDL,DrieverW,etal.Gridlock,alocalizedheritablevascularpatterningdefectinthezebrafish[J].NatureMedicine,1995,1(11):11431147.[13]GoldsmithP.Modellingeyediseasesinzebrafish[J].Neurorepor,t2001,12(13):A7377.[14]MartinaF,HelmutS.Aromatasemodulationaltersgonadaldifferentiationindevelopingzebrafish(Daniorerio)[J]GonadotropinReceptor,GeneralandComparativeEndocrinology,2002,125:349364.16