Unicorn Method Editor

ÄKTATraining,June,20031MethodEditor方法编辑ÄKTATraining,June,20032MethodEditor方法编辑•功能：–编辑方法(MethodWizard,RunSetup,TextInstruction)–编辑层析柱列表(ColumnList)–编辑缓冲液配方(BufferPrepReceipt)–……ÄKTATraining,June,20033MethodEditor方法编辑•MethodEditor–MethodWizard•运用模板快速编程–RunSetup•编辑运行参数等–TextInstruction•编辑运行程序•ColumnList•BufferPrepReceiptÄKTATraining,June,20034MethodWizard——启动ÄKTATraining,June,20035MethodWizard——层析技术、层析柱选择分离技术选择层析柱选择是否使用缓冲液自动配置选择缓冲液配方设定缓冲液pHÄKTATraining,June,20036MethodWizard——检测波长等选择检测波长起始B液浓度平衡体积ÄKTATraining,June,20037MethodWizard——进样方式上样体积ÄKTATraining,June,20038MethodWizard——收集方式ÄKTATraining,June,20039MethodWizard——收集方式•Frac-900orFrac-950–用收集器收集，设置每管收集体积•OutletValves–用收集阀收集，设置每个阀位收集的体积•PeakFractionation–按峰收集，设置峰识别参数，如level,峰高，或Slope，峰斜率等ÄKTATraining,June,200310MethodWizard——洗脱方法ÄKTATraining,June,200311MethodWizard——洗脱方法•IsocraticÄKTATraining,June,200312MethodWizard——洗脱方法•IsocraticwithDelayedFractionationÄKTATraining,June,200313MethodWizard——洗脱方法•LinearGradientÄKTATraining,June,200314MethodWizard——洗脱方法•SegmentedGradientÄKTATraining,June,200315MethodEditor方法编辑•MethodEditor–MethodWizard•运用模板快速编程–RunSetup•编辑运行参数等–TextInstruction•编辑运行程序•ColumnList•BufferPrepReceiptÄKTATraining,June,200316RunSetupÄKTATraining,June,200317RunSetup——Variable可变参数ÄKTATraining,June,200318RunSetup——Scouting（探索）Scouting功能：条件优化重复制备ÄKTATraining,June,200319RunSetup——Notes记录ÄKTATraining,June,200320RunSetup——Questions提问提问问题回答方式默认答案ÄKTATraining,June,200321RunSetup——Gradient梯度曲线ÄKTATraining,June,200322RunSetup——BufferPrep缓冲液配方校正因子母液ÄKTATraining,June,200323RunSetup——Columns层析柱参数ÄKTATraining,June,200324RunSetup——ReferenceCurves参考曲线1，选择文件名2，选择参考曲线3，确认ÄKTATraining,June,200325RunSetup——EvaluationProcedures结果处理程序ÄKTATraining,June,200326RunSetup——MethodInformation信息ÄKTATraining,June,200327RunSetup——ResultName结果命名ÄKTATraining,June,200328RunSetup——StartProtocol启动方案ÄKTATraining,June,200329RunSetup——Frac-950收集器设置ÄKTATraining,June,200330MethodEditor方法编辑•MethodEditor–MethodWizard•运用模板快速编程–RunSetup•编辑运行参数等–TextInstruction•编辑运行程序•ColumnList•BufferPrepReceiptÄKTATraining,June,200331TexInstruction——程序结构ÄKTATraining,June,200332TexInstruction——Block程序块•Block结构程序块名断点（Breakpoint）单位命令行结束句ÄKTATraining,June,200333TexInstruction——命令行组成断点操作命令变量值变量名ÄKTATraining,June,200334MethodEditor方法编辑•MethodEditor–MethodWizard•运用模板快速编程–RunSetup•编辑运行参数等–TextInstruction•编辑运行程序•ColumnList•BufferPrepReceiptÄKTATraining,June,200335ColumnListÄKTATraining,June,200336ColumnList点击“Edit”键，启动柱参数编辑窗口。ÄKTATraining,June,200337ColumnList点击“New”键，启动新柱编辑窗口。“mand”为必须输入的参数。ÄKTATraining,June,200338MethodEditor方法编辑•MethodEditor–MethodWizard•运用模板快速编程–RunSetup•编辑运行参数等–TextInstruction•编辑运行程序•ColumnList•BufferPrepReceiptÄKTATraining,June,200339BufferPrepRecipesÄKTATraining,June,200340BufferPrepRecipesÄKTATraining,June,200341BufferPrepRecipesÄKTATraining,June,200342BufferPrepRecipes