两种有机硅表面活性剂和3种农药对斑马鱼的急性毒性与联合毒性研究

2009,11(1):1452148ChineseJournalofPesticideScience3,,,,,,3(,310021):,(,JIERUN)(,High2Speed)3:LC50(96h)5.9017.4mg/L,139.52107.24165.65,;111.3648.2680.08,:;;;;;;:S481.3:A:100827303(2009)0120145204AcuteandJointToxicityofTwoOrganosiliconSurfactantswithThreePesticidestoBrachydaniorerioWUSheng2gan,WUChang2xing,CHENLi2ping,CANGTao,YURui2xian,WANGYan2hua,ZHAOXue2ping3(InstituteofQualityandStandardforAgro2products,ZhejiangAcademyofAgriculturalSciences,Hangzhou310021,China)Abstract:Inordertoassesstheeffectoforganosiliconsurfactantonaquaticorganisms,theacuteandjointtoxicityofethoxymodifiedtrisiloxane(JIERUN)andsiloxanecompound(High2Speed)withthreepesticideswerestudiedusingzebrafish(Brachydaniorerio)astestorganismbysemi2staticmethod.TheresultsshowedthatJIERUNandHigh2Speedweremiddle[5.90mg(a.i)/L,96h2LC50value]andlowtoxicity[17.4mg(a.i)/L,96h2LC50value]tozebrafish,respectively.TheCo2ToxicityCoefficient(CTC)ofJIERUNwithfipronil,indoxacarbandacephatetozebrafishwas139.52,107.24and165.65,respectively,sothejointactionwassynergistic,additiveandsynergistic,respectively.TheCTCofHigh2Speedwithfipronil,indoxacarbandacephatetozebrafishwas111.36,48.26and80.08respectively,sothejointactionwasadditive,antagonisticandadditive,respectively.Keywords:organosiliconsurfactant;fipronil;indoxacarb;acephate;zebrafish(Brachydaniorerio);acutetoxicity;jointtoxicity:2008208206;:2009201231.:(19772),,,,,E2mail:[e-mail];3(Authorforcorrespondence):(19692),,,,.:0571286404229;E2mail:[e-mail]:(2006C12023).Vol.112060,80,,[1],,,[2],SilwetL277Phyllo2cnistiscitrellaMyzuspersicae,TetranychuspacificusAphisgossypiiFrankliniellaoccidentalis[35],,BBemisiatabacPlutellaxylostellaL.,[3,6,7],,3,3[810],[1115],,3,11.1Brachydoniorerio(,2.53.5cm,0.20.4g,)714d,5%1,,24h1.2100%(,JIERUN),;100%(,High2Speed),GE50g/L(fipronil)SC,;150g/L(indoxacarb)SC,;30%(acephate)EC,1.31.3.1,24h[16]3,5,24487296h,LC5095%[17]1.3.2,(),(3102.710185,,),(3102.710185)61.3.11.4(CTC)()[18]CTC120,CTC80,CTC80120[19]22.152h,,;,,,,,2.21,LC50(96h)5.9017.40.7460.37818.2mg/L5:[16],,,2.2(2):641No.1:315Table1TheacutetoxicityoffivetestedagrochemicalstozebrafishTestedagrochemicalsTimeofexposure/hLC50/(mg/L)95%CL/(mg/L)Toxicitycategory(96h)JIERUN245.905.666.16Middle485.905.666.16725.905.666.16965.905.666.16High2Speed2423.921.226.8Low4819.8-7219.8-9617.415.719.4fipronil242.832.453.27High481.291.111.51720.8730.7301.04960.7460.6130.908indoxacarb24--High480.4730.4250.526720.4090.3660.456960.3780.3390.421acephate2432.127.737.1Low4823.420.127.37220.717.824.19618.215.920.92Table2ThejointtoxicityofJIERUNandHigh2Speedwithfipronil,indoxacarbandacephatetozebrafish()Testedagrochemicals(m/m)Timeofexposure/hLC50/(mg/L)95%CL/(mg/L)CTC(96h)Jointaction(96h)+243.00-139.52fipronil+JIERUN(310)482.151.902.44Synergistic721.841.582.14961.631.381.93+242.522.113.00107.24indoxacarb+JIERUN(2.710)482.331.982.73Additive721.631.322.03961.341.061.70+2412.110.314.3165.65acephate+JIERUN(185)489.578.0111.4Synergistic729.207.7810.9967.566.249.17+242.982.683.32111.36fipronil+High2Speed(310)482.982.683.32Additive722.752.453.08962.542.272.84+245.264.665.9548.26indoxacarb+High2Speed(2.710)484.353.834.95Antagonistic724.243.714.84963.413.053.83+2456.552.061.380.08acephate+High2Speed(185)4840.837.045.1Additive7230.326.434.99622.518.827.0741Vol.11,LC50(96h)1.631.347.56mg/L,139.52107.24165.65,LC50(96h)2.543.4122.5mg/L,111.3648.2680.08,,3,3,,3,,,[810],,,,[13,15],,,,,,:[1]WANGYu2hua(),LIYao(),SHEHui2ling(),etal.[J].SiliconeMaterial(),2005,19(1):527.[2]HUANai2zhen(),LINYu2jia().[J].JiangsuChemicalIndustry(),2000,28(4):20221.[3]SHAPIROJP,SCHROEDERWJ,STANSLYPA.BioassayandEfficacyofBacillusthuringiensisandanOrganosiliconeSurfactantagainsttheCitrusLeafminer(Lepidoptera:Phyllocnistidae)[J].FloridaEntomologist,1998,81(2):2012210.[4]IMAIT,TSUCHIYAS,FUJIMORIT.AphicidalEffectsofSilwetL277,OrganosiliconeNonionicSurfactant[J].AppliedEntomologyandZoology,1995,30:2,3802382.[5]TIPPINGC,BIKOBAV,CHANDERGJ,etal.EfficacyofSilwetL277againstSeveralArthropodPestsofTableGrape[J].SourceJournalofEconomicEntomology,2003,96(1):2462250.[6]ZHANGJin2liang(),YUANZhi2qiang(),YANGJian2guo(),etal.B[J].BeijingAgriculture(),2007,(2):9210.[7]LIYa2zhen(),TAOYan2hua(),TANGHai2yan(),etal.GE[J].ShanghaiVegetables(),2006,(6):70271.[8]LIUChang2ling().[J].Pesticides(),2003,42(2):42244.[9]ZHAOYu2ding(),WANGDong2lan(),LAIYou2peng(),etal.[J].PesticSciAdmin(),2008,29(8):25229.[10]SHIJian2jun(),ZHUANGHai2bing(),WANGZhi2zheng(),etal.3[J].JZhejiangOceanUniv(NatSci)(,),2006,25(2):1292132.[11]SUNHan2chang(),HUANGLi2ying(),DINGShi2hua(),etal.SDS[J].ChineseAgricSciBull(),2005,21(11):4062408.[12]ZHENGYan2jing(),CHENLin(),CHENYan2ping(),etal.[J].JAgro2EnvironSci(),2006,25(Suppl.):4962498.[13]ZENGXiao2mei(),CHENZhi2gang().[J].YunnanEnvironSci(),2005,24(1):729.[14]SIBILAMA,GARRIDOMC,PERALESJA,etal.EcotoxicityandBiodegradabilityofanAlkylEthoxysulphateSurfactantinCoastalWaters[J].ScienceoftheTotalEnvironment,2008,394:2652274.[15]BOILLOTC,PERRODINY.Joint2actionEcotoxicityofBinaryMixturesofGl