您好,欢迎访问三七文档
当前位置:首页 > 医学/心理学 > 药学 > 中国生命科学论坛精华-生物谷-生物医药门户
PCR1111Primer3://=25&ID=38536Oligo6.67=25&ID=408981.PCR1.1824242.GC4060GCGC3.5'GC4.3',?G?:?G0~-2,PCR100%,?G=-6,40%.5.3'GC53AT6.3'3'3’GCAT7.8.Tm50DNATm5Tm(Wallace):Tm=4g+C+2a+T,15-20,(Baldino)14-700.4mol\LTm,Tm.Tm=81.5+16.6*lg[K+]+0.41(%[G+C])-(675/n)TmGGGAAGAGAGTmTmPrimer5PCR12115'Tm10.3'3'3PCR1µM3µM,:,,!,”ATGTGA”ATGTCA5’50OD1OD2OD1ODHPLCOPCµgDNA3×1043×105,0.1gDNA,10ngDNA,1ngDNA.,.PCR,,DNA..DNAPCR2.TE100µMTEpHTEPCRddH2O-2010µMTE-20615301-201153020.10.5µM’A3’-5’TATaqATaqDNA3'5'TaqDNA4.Mg2PCRDNAdNTP200µMdNTPPCR1.5mMPCR35mM,PCR14115.dNTPsdNTPsdNTP200µM25-50µM6.KCl50mmol/L,70-100mmol/L.PCR1.,945-10min,DNA,TaqDNA(hotstart),,PCR,DNA,DNA.941min,DNA,GC,.2.PCR5570Tm5,,2TmTm5Tm5Tm,,(6094),,3.72,TaqDNA75.,,TaqDNA20-85..,TaqDNA1kbDNA.,,(10-30min),,7210min=95&ID=471014.,DNA25-30,PCR25-30,TaqDNA,,,DNA103-105,,60,109-1010,:CC0(1+P)nC,C0DNA,P,n,,,,PCR1511PCR1.PCRTouchDownPCRPCRPCRTm51212Tm5PCRAFLP®DNA2.PCRPCRTaqDNA72PCRDNAPCRTaqDNAPCRPCRDNAPCRTaqDNA3.PCRGCDNAPCRDMSOPCRxEnhancerSolutionDNAPCRxSolutionPlatinumTaqDNAPlatinumPfxDNAPlatinum-TaqDNADMSO4.PCR152010010001520PCRPCR16113040PCRmRNAPCR5'RACEPCRPCR:1.DNA:PCR,,,,,,,.,,.2.PCR:,,.PCRPCR.1():;;.()PCR:PCRPCR.()PCR.PCR.PCR(1013/ml)PCRPCR.PCR;.48000.():...2.1.:PCRPCRPCR.(100)..PCR.PCR17112.:PCR.:;.:PCRDNARNAPCR.3.4.PCR3():PCRPCRPCRPCR.;PCR;PCR;PCR..DNARNA.():..()PCR:PCRPCR-20..PCRPCRPCR.().().()PCRPCR.()Eppendorf..4PCRPCRPCRPCRPCRPCR1.2.PCRPCR3.PCR??PCRDNADNA1PCRPCR18112dNTPPCR3dNTP()1.2.PCR3.4.dNTP5.6.PCR7.DNAPCRPCR8.100PCRSourthernPCRPCRDNAPCR1.2.3.4.5.6.7.120.1ml35mlPCR48.PCR1911PCR1.1mol/LDNA2.UVnm254/300nm30minUVPCRPCRUV500bpUVPCRDNAUVUVDNA0.065/DNADNATaqDNA0.13/DNA500bpDNA32105100bp6105UV1.DNaseIPCRTaq0.5UDNaseI30minTaqPCRDNA2.4MspITaqI1hPCR3.TaqPCRUV4.g1.5kGy0.1ngDNA2.0kGy1044.0kGyPCRPCRPCRgDNAUNGUV500bpPCR300bpUNG1.PCRdUdTdUPCRUNGUDGN-dUTaqDNAUNGdUUNGDNARNA2.dUTPdUTPdTTPdUPCRUNGPCRPCRUNGPCRdUPCR3.dUdUdTPCR5dUUNG1-2kbdUTPdTTPdUdUdU34.UNGPCRPCR11011dU5.dUTPDNAPCRUNG-UNGUNG1RNA23RNA2PCRRS-PCRRNA-specificPCRPCRRNAPCRPCR3A20520CmRNAcDNACcDNAcDNAPCRBCcDNADNADNADNADNAPCR11111:,JB=CorGorTD=AorGorTH=AorCorTK=GorTM=AorCN=AorCorGorTR=AorGS=CorGV=AorCorGW=AorTY=CorTPCRPCRRT-PCRAP-PCRPCRPCRinvitrogenPCRRT-PCR3PCRFootball20002Gaoyx
本文标题:中国生命科学论坛精华-生物谷-生物医药门户
链接地址:https://www.777doc.com/doc-348496 .html