亲水性分子印迹纳米球的合成及药物识别与释放性能研究

49320105()ACTASCIENTIARUMNATURALIUMUNIVERSITATISSUNYATSENIVol149No13May201031,2(11,510642;21,510006):s-(T),(MAA),(GMA),(DVB),n(T)n(MAA)n(GMA)n(DVB)=14424,,832nm,,:s-;;;:O632:A:0529-6579(2010)03-0061-05PreparationofHydrophilicMolecularlyImprintedNano2spheresandthePropertiesontheDrugReleaseandRecognitionXULi1,HEJianfeng2(11CollegeofSciences,SouthChinaAgriculturalUniversity,Guangzhou510640,China;21CollegeofChineseTraditionalMedicine,GuangzhouUniversityofChineseMedicine,Guangzhou510006,China)Abstract:Theorbicularmolecularlyimprintedpolymer(OC2MIP)waspreparedbytwostepsincludingtheprecipitationpolymerizationandtheopeningtheGMAepoxidering.ThenthehydrophilicexternallayeronthesurfaceofOC2MIPwasformed.TheOC2MIPwascharacterizedbyinfraredspectraandscan2ningelectronmicroscopy(SEM).WaterabsorptionmeasurementshowedtheOC2MIPhadstrongerwatercompatiblethanMIP.WhentheproportionofMAA/GMAwas11,andDVBis3timesofMAAcoGMA,theparticleshapesofMIPswereuniformandtherecognitionandreleaseofOC2MIPinpolarsol2ventweresatisfactory.TheresultsshowedGMAaddedinthepolymerscouldadjustthecapabilityofre2leaseofthepolymerscertainly.AftertheGMAepoxideringwasopened,OC2MIPsynthesizedwasmoresuitabletobeemployedinbiologicalmediabecauseofthereductionofnon2specifichydrophobicinterac2tions.Inaddition,thepolymershadthecapabilityofrecognition,alsocouldsatisfythestudyofbothdrugrecognitionandsustained2releaseinpolarsolvent.Keywords:s2naproxen;precipitationpolymerization;molecularlyimprintedpolymers;recognitionandrelease3:2009-08-24:(K5090006);(4900-K04162):(1972),,;E2mail:lindafeng_xul@1631com()49,(molecularlyimprintedpolymer,MIP)[1-5]MIP,,,,MIP,,,,,,,,[6-7]S-(+)-,,,,,,,1S-(,Fluka);-(MAA,,);(GMA,,);(DVB,,)MAA,GMA,DVB(,,);(UV-2550),AVATAR360FT2IR(KBr)(Nicolet),(Agilent1100),C18(415mm150mm,Agilent)XL230ESEM();Zetasizer3000HS(Malvern,UK)2211[8]s-012306g,013443gMAA,,10min,,015686gGMA311243gDVB40mL(/73),5min50mg,5min,,6524h,,24h,60MIP3100gMIP,20mL=10%,24h,60OC2MIP(),(NIP)(OC2NIP)21221211,50mg(1cm6cm),,mq,011mol/LHCl(pH1)(pH618),,,mh:m(H2O)=((mh-mq)/mq)100%,mhmq2121220mg,10mL,310mL3mmol/L(=90%+=10%),24h,HPLC:/,73;245nm;1mL/min;20L21213OC2MIP50mg,,3mmol/L715mL,,103060120180240min,1mL(1mL)0145m,HPLC,21214OC2MIP50mg,,715mL,4h,,HPLC,,MIPpH615715mL,,HPLC263:15244872100h,3311(1),MAA,GMA,,,2,1OC2MIPFig11SchematicillustrationofOC2MIPpreparation,,,[9-10](2),832nm2OC2MIPFig12ScanningelectronmicroscopyofOC2MIP3MIPOC2MIP,3,OC2MIP1120cm-1C2O,C2O,C2O3MIP(a)OC2MIP(b)Fig13IRspectraofMIP(a)andOC2MIP(b),(1)1,OC2MIPMIP,(),,OC2NIPNIP,OC2MIP,,,1Table1Absorptionratiosofwaterandimprintedmoleculesbypolymers/%pH=011pH=618/%MIP51131382112NIP6135176211OC2MIP391543138411OC2NIP43135012511736()493124,Y=195107565+2310363X(R=0199533)5OC2MIPOC2NIP,5,,60min74%,42%,90min,100min,78mg/g,56mg/g,313MIP21314OC2MIPOC2NIPpH6156,6,OC2NIP10h,OC2MIP1h,OC2MIP3817%,,,10h6313%,,,,,,,6Fig16Releaseprofilesofs2naproxenfromOC2MIPandOC2NIP4MAA,GMA,DVB,,,,OC2MIPn(T)n(MAA)n(GMA)n(DVB)=14424OC2MIPOC2MIP,,:[1]CAROE,MARCbRM,BORRULLF,etal.Applica2tionofmolecularlyimprintedpolymerstosolid2phaseex2tractionofcompoundsfromenvironmentalandbiologicalsamples[J].TrendsAnalChem,2006,25(2):143-154.[2]SUEDEER,BODHIBUKKANAC,TANGTHONGN,etal.Developmentofareservoir2typetransdermalenantio2selectivecontrolleddeliverysystemforracemicpropranololusingamolecularlyimprintedpolymercompositemembrane[J].JControlledRelease,2008,129(3):170-178.(69)463:,HSCCC,,,,:[1]ASHIHARAH,KATOM,YECX,etal.Biosynthesisandmetabolismofpurinealkaloidsinleavesofcocoatea[J].JPlantResearch,1998,111(4):599-604.[2]ZHENGXQ,YECX,KATOM,etal.Theacrine(1,3,7,92tetramethyluricacid)synthesisinleavesofaChinesetea,kucha(Camelliaassamicavar.kucha)[J].Phytochemistry,2002,60(2):129-134.[3],,,.[J].:,2008,47(6):129-130.[4]XUJK,KURIHARAH,ZHAOL,etal.Theacrine,aspecialpurinealkaloidwithsedativeandhypnoticproper2tiesfromCamelliaassamicavar.kuchainmice[J].JournalofAsianNaturalProductsResearch,2007,9(7):665-672.[5],,,.[J].:,1999,38(5):82-86.[6]YANGXR,YECX,XUJK,etal.Simultaneousanaly2sisofpurinealkaloidsandcatechinsinCamelliasinensis,CamelliaptilophyllaandCamelliaassamicavar.kuchabyHPLC[J].FoodChemistry,2007,100(3):1132-1136.[7]XUJK,ZHANGWK,SUYB,etal.Chemicalconstitu2entsfromtheleavesofCamelliaassamicavar.kuchaChangetWang[J].ChineseJournalofNaturalMedi2cines,2009,7(2):111-114.[8],,,.[J].,2001,29(5):586-591.[9],,,.[J].,1998,18(1):60-65.[10],,,.[J].,2007,25(4):528-531.[11],.[J].,2007,25(5):735-739.[12],,,.[J].,2009,27(2):244-247.[13],,,.[J].,2006,16(4):78-80.(64)[3]PRASADBB,SHARMAPS,LAKSHMID.Molecularlyimprintedpolymer2basedsolid2phaseextractioncombinedwithmolecularlyimprintedpolymer2basedsensorforde2tectionofuricacid[J].JChromatogrA,2007,1173(1-2):18-26.[4]SCHWEITZL,ANDERSSONLI,NILSSONS.Rapidelectrochromatographicenantiomerseparationsonshortmolecularlyimprintedpolymermonoliths[J].AnalChimActa,2001,435(1):43-47.[5],,,.[J].:,2005,44(3):58-62.[6],.[J].,2006,29(2):82-84.[7],,,.[J].,2006,22(4):19-23.[8]FRANCESCOP,FRANCESCAI,GIUSEPPEC.Newre2strictedaccessmaterialscombinedtomolecularlyimprin2tedpolymers