植物花精油的提取方法和生物活性研究进展

2120132瑑瑥1212121212121．4101282．410128、、、CO2、。。TS202．3A1007－7561201302－0035－04ResearchprogressonextractionoffloweressentialoilandtheirbiochemicalactivityHUANGXin－an12TANXing－he12ZHANGYu12WANGFeng12CAIWen12XUNing121．CollegeofFoodScienceandTechnologyHunanAgriculturalUniversityChangshaHunan4101282．KeyLaboratoryofFoodScienceandBiologicalTechnologyofHunanProvinceChangshaHunan410128AbstractTheextractionmethodsforfloweressentialoilsindomesticandabroadatpresentwerere-viewedincludingsteamdistillationsimultaneousdistillationandextractionorganicsolventextractionsupercriticalCO2extractionandsolidphasemicro－extraction．Theresultwasbetterifcombiningtowormoreextractionmethodstoextracttheessentialoil．Theirbiologicalactivitywassummarizedinordertoprovidetheoryreferenceforthefurtherresearch．Keywordsfloweressentialoilsextractionbiologicalactivity2012－08－312009XK6001－51989．1959．essentialoils、、、、、、。、、、。、。、、、1－2。、。。1、、。、、3。、、、CO2、。1．1SteamdistillationSD。2120132瑑瑦。。。480～1001∶1410h13h0．96%0．01%～0．03%。1．2Simultaneousdistillationex-tractionSDE。。。Omata5P．rubra‘IrmaBryan’GC—MS67β－、620g600mL30min50mL45℃4h。0．57%70．41%。。1．3SolventextractionSE。。。81∶10、58℃、7h3。2．85%2．57%1．95%。0．16%。。1．4CO2CO2CO2supercriticalfluidextractionSFECO2、。、、。9。。Scalia．S10CO29119kPa、40℃CO230min4h4．411CO212MPa35℃、1．5h、CO24．0kg/h1．08%43．2。1．5SolidphasemicroextractionSPME1。、、、、。MiSookChung12hallabong28．89%－43．24%、0．39%－23．06%、β－0．38%－16．67%、1．37%－11．42%、4－1．26%－6．12%13GC－MS1375873．48%1066291．29%。、。1．6。1．6．1Microwaveacceleratedextrac-tionMAE、14。2120132瑑瑧、15。。F．Chemata16L．angustifolia。8．86%8．75%。。。。、、17。。18595W60min。0．80%0．68%。1．6．2Ultrasound－assistedextrac-tionUSE。。1。Alissandrakisa19411．3%51．6%80．6%75．2%203．0h1．0h3．0h0．32%0．24%。1．6．321－22。。23151．61V45．16s1∶9．770．1756%240．056%。。2、、、、、、。2．125、、、。、、、、。6－－56－－2－44．28%。AlvarezC26177－2．2．1－2－、1R4R－177－2．2．1－2－。2．227。0．4mg/kg。6．548%42．506%28。、、。2．329DPPHβ－/DPPH。β－16．06%、14．65%、β－9．26%、β－6．42%。2．430Wistar0．5%2120132瑑瑨3d0．5%。。19．293%8．664%4．661%710－4．384%31。3。、。1．N．2011264118－121．2MacielMVMoraisSMBevilaquaCMLetal．Chemicalcomposi-tionofEucalyptusspp．essentialoilsandtheirinsecticidaleffectsonLutzomyialongipalpisJ．VeterinaryParasitology20101671－7．3PenuelasJLlusiaJ．BVOCsplantdefenseagainstclimatewarningJ．TrendsinPlantScience20038105－109．4．J．201225－7．5OmataANakamuraSHashimotoSetal．Volatilecomponentsofplumeriaflowers．Part2．PlumeriarubraL．cv．‘IrmaBryan’J．FlavourandFragranceJournal19927133－35．6．GC－MSN．2012312182－186．7．N．20062671478－1481．8·．3N．2011342162－167．9YANYouchunBORNSCHEUERUTSTADLERGeta1．Pro-ductionofsugarfattyacidestrsbyenzymaticesterificationinastirred－tankmembranereactoroptimizationofparametersbyresponsesurfacemethodologyJ．JournaloftheAmericanOilChemistsSociety2001782l147－1153．10ScaliaSGiuffredaLPalladoP．Analyticalandpreparativesuper-criticalfluidextractionofChamomileflowemanditcomparisonwithconventionalmethodsJ．JournalofPharmaceuticalandBiomedicalAnalysis199921549－55811．CO2J．200618663－666．12MiSookChung．VolatileCompoundsoftheHallabongCitruskiyomi×CitrusponkanBlossomJ．FoodSci．Biotechnol．2012211285－290．13．J．20071871579－1582．14．J．2006294387－389．15LUCCHESIMECHEMATFSMADJAJ．AnoriginalsolventfreemicrowaveextractionofessentialoilsfromspicesJ．FlavourandFragranceJournal2004192134－138．16F．ChemataM．E．LucchesiJ．Smadjaetal．Microwaveacceler-atedsteamdistillationofessentialoilfromlavenderArapidcleanandenvironmentallyfriendlyapproachJ．AnalyticaChimicaActa2006555157－160．17WANGZMDINGLLITCetal．Improvedsolvent－freemi-crowaveextractionofessentialoilfromdriedCuminumcyminumL．andZanthoxylumbungeanumMaximJ．JournalofChromatog-raphyA200611021/211－17．18．GC/MSN．2009304244－249．19AlissandrakisaEDafererabDTarantilisPAetal．Ultrasound－as-sistedextractionofvolatilecompoundsfromcitrusflowersandcitrushoneyJ．FoodChemistry200382575－582．20．J．2011392917862－17864．21DengYMCuiYMLiWeta1．OptimizationforHomogenateEx-tractionofTotalFlavonoidsfromGlycyrrhizaLeavcsbyResponseSur-faceMethodJ．Chemistry＆Bioengineer200925944－47．22．J．200911334－36．23．J．2011275483－488．24．J．200943632－34．25．J．2011392817244－17245．26AlvarezCPinoPBChrisDetal．Antifungalactivityoftheessen-tialoilofflowerheadsofgarlandChrysanthemumChrysanthemumcoronariumagainstagriculturalpathogensJ．Phytochemislry200157799－104．27．N．2009372126－129．28．GC－MSJ．200735288776－8798．29．J．2010213622－625．30．J．2011304449－451．31．CO2J．2009312212－216．●