基因芯片在环境毒理学研究中的应用进展

©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(,116011)DNA1cm2,,,,29AdvanceingenechipapplicationinenvironmentaltoxicologyNiYuwenLiangXinmiaoChenJipingZhangQing(DalianInstituteofChemicalPhysics,ChineseAcademyofSciences,Dalian116011)AbstractGenechipmayintegrateabundantinformationofDNAwithina1cm2arrayandhaveanabilitytodealwithmassivelife2informationsimultaneously.Itprovidesavalu2ableplatformforenvironmentaltoxicologyresearch.Genechipmaybeusednotonlytoaccu2ratelyperformtheanalysisofhumangeneexpressiononwhichcontaminationshaveaneffectbutalsotocategorizeandclassifycontaminations.Progressively,itcanbeusedtoscreentox2icanttargetsandtoclarifythemechanismofactionofcontaminations.Genechipapplicationinthefieldoftoxicologyaresummarizedinthepaper.The29referencesarereviewed.Keywordsgenechip;environmentaltoxicology;contaminations,,DNA,DNA/[1,2],2080SBH(sequencingbyhybridization)[3],2090,10,24Vol.2,No.420018TechniquesandEquipmentforEnvironmentalPollutionControlAug.,2001©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:[4,5];[6]DNA,DNA,,DNA5,5,,n,4n4n,Affymetrix6500DNA,5000001000000DNA,,mRNADNA,cDNA[6]2,[7],,[8],,,(PNA)[9]PNA,DNA,,PNA2DNA,3[7,10],CCD,,;452©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(),,,,Watson2Crick,,,4,[12],,NIEHs:(1);(2);(3);(4);(5);(6);(7);(8);(9)[11]4.1(),[13],113cDNA(peroxisomeproliferator(a))[14],4.2cDNA,DNADNA(),,[15,16],554:©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[12],cDNA(IR),RNA[17]4830IR,IR12,IR,,[18],[19]cDNA[20]cDNADNA,,,[18],[21]8hcDNA47,28(IL28)7HepG228mRNA,28,,652©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[12],,,RNA,,PAHsperoxisomepro2liferators[18],,4.4,,,,,,[22],,PennieToxBlot,[23]ToxBlot(insulin2sensitizing)NIEHs,ToxChipv1.0ToxChipv2.0[18]ToxChipv1.02090ToxChip,,,,[24],,DNAbeta2NF[25]P4501a1P4501a2beta2NF8h,P4501a210597cDNAHeLaBPDE[26],0.4MBPDE53h,9,3754:©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[27],NIEHsPAHs,,,[28,29],,,,[14]5,10DNA,,,,,,1000001cm2,,,,,[1]MarshallA,HodgsonJ.DNAchips:anarrayofpossi2bilities.Nat.Biotec.,1998,16:2731[2]RamsayR.DNAchips:state2of2the2art.Nat.Biotec.,1998,16:4044[3]DrmanacR,LabatI,BruketI,etal.SequencingofmegabaseplusDNAbyhybridization:theoryofthemethodgenocics.Genomics,1989,4:114128[4]KovacsGTA,PetersenK,AlbinM.Silisonmicromach2ingsensorstosystems.Anal.Chem.News&features,1996,7:407a412a[5]StephenPA,FodorJ,LeightonR,etal.Light2direct2ed,spatialladdressableparallelchemicalsynthesis.Sci2ence,1991,251:767772[6]RodaA,GuardigliM,RussoC,etal.Proteinmicrode2positionusingaconventionalink2jetprinter.Biotech,2000,28(3);492496[7][8],.,:,2000,68[9][10]ShalonD,SmithJS,BrownPO.ADNAmicroarraysystemforanalyzingcomplexDNAsamplesusingtwo2colorfluorescentprobehybridization.GenomeRes.,1996,6(7):639645[11]AfshariCA,NuwaysirEF,BarrettJC.ApplicationofcomplementaryDNAmicroarraytechnologytocar2cinogenidentification,toxicology,anddrugsafetyeval2uation.CancerResearch.,1999,59(19):47594760[12]ChhabraRS,HuffJE,SchwetzBS,SelkirkJ.Anoverviewofprechronicandchronictoxicitycarcino2genicityexperimental2studydesignsandcriteriausedbythenationaltoxicology.Pro.EnvironHealthPer2852©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:313321[13]CooneyCM,Analyzingtoxicantsonachip.Environ.Sci.Tec.,2000,244A245A.[14]CunninghamMJ,ZweigerGB,PanzerSR,etal.Toxicitytestingusingamicroarrayofnucleicacidtoxi2cologicalresponsemarkers.PCTInt.Appl.,2000,5:76.[15]BrownPO,andBotsteinD.ExploringthenewwordofthegenomewithDNAmicroarrays.Nat.Genet.,1999,21(supplement):3337[16]DugganDJ,BittnerM,ChenY,MeltzerP,TrentJM.ExpressionprofilingusingcDNAmicroarrays.Nat.Genet.,1999,21(supplement):1014[17]AmundsonSA,BittnerM,ChenYD,TrentJ,etal.FluorescentcDNAmicroarrayhybridizationrevealscomplexityandheterogeneityofcellulargenotoxicstressresponses.Oncogene,1999,18(24):36663672[18]NuwaysirEF,BittnerM,TrentJ,BarrettJC,Af2shariCA.Microarraysandtocicology:theadventoftoxicogenomics.Mol.Carcinogen.,1999,24(3):153159[19]MartonMJ,DerisiJL,BennettHA,etal.Drugtar2getvalidationandidentificationofsecondarydrugtar2geteffectsusingDNAmicroarrays.Nat.Med.,1998,4(11):12931301[20]DebouckC,GoodfellowPN.DNAmicroarraysindrugdiscoveryanddevelopment.Nat.Genet.,1999,21(supplement):4850[21]PeterRH,NeilHJ,AN.Brooks,etal.Identifica2tionofapossibleassociationbetweencarbontetrachlo2ride2inducedhepatotoxicityandinterleukin28expres2sion.J.Biochem.Mol.Toxic.,2000,14:283290[22]HaugenA.Progressandpotentialofgeneticsuscepti2bilitytoenvironmentaltoxicants.ScandJ.WorkEnvi2ronHealth,1999,25(6):537540[23]PennieWD,TugwoodJD,OliverGJA,KimberI.Theprinciplesandpracticeoftox