鱼类转基因技术

2001,Vol.21,No.3(,210097)1,,,,,,80,,20,,[28][4][8][9],(1),1(P)Carassiusauratus(Goldfish)mMTPhGH,1985Misgurnusanguillicaudatus(Loach)mMTPhGH,1986Oryziaslaticeps(Medaka)SV40PcCrOzatoetal.,1986Oncorhynchusmykiss(Trout)SV40PhGHChourroutetal.,1986Ictaluruspunctatus(Catfish)mMTPhGHDunhametal.,1987Oncorhynchusmykiss(Trout)mMTPrGHMacleanetal.,1987Salmosalar(Salmon)mMTPhGHRokkonesetal.,1989Salmosalar(Salmon)fAFPPfAFPFletcheretal.,1988Brachydaniorerio(Zebrafish)SV40PHygroStuartetal.,1988Salmosalar(Salmon)mMTP2GalMcEvoyetal.,1988Salmosalar(Salmon)mMTPrGHMcEvoyetal.,1988Oreochromisniloticus(Tilapia)mMTPhGHBremetal.,1988Brachydaniorerio(Zebrafish)SV40PCATStuartetal.,1988Oryziaslaticeps(Medaka)mMTPrtGHInoueetal.,1990Oryziaslaticeps(Medaka)FLusPFLucTamiyaetal.,1990Oncorhynchusmykiss(Trout)cCPcCOshiroetal.,1989Carassiusauratus(Goldfish)RSVPCATHallermanetal.,1990Cyprinuscarpio(Commoncarp)RSVPrtGHZhangetal.,1990EsoxamericanusGmelin(Pike)RSVPbGHGuiseetal.,1991Misgurnusanguillicaudatus(Loach)mMTPhGHBenyumovetal.,1989Ictaluruspunctatus(Channelcatfish)RSVPbGHSchneideretal.3Cyprinuscarpio(Commoncarp)SV40PbGH,1988Cyprinuscarpio(Commoncarp)RSVPbGH,1988Carassiusauratus(Goldfish)RSVPNeoYoonetal.,1990Oncorhynchusmykiss(Trout)RSVPtGH(cDNA)Powersetal.,1990Ictaluruspunctatus(Catfish)RSVPrtGHPowersetal.,1991Cyprinuscarpio(Commoncarp)mMTPhGHPowersetal.,1991Cyprinuscarpio(Commoncarp)mMTPhGHHernandezetal.,1991Misgurnusanguillicaudatus(Loach)mMTPhGHXieetal.,1989Cyprinuscarpio(Commoncarp)mMTPhGHXieetal.,1993032,;,,;,,,,,,3mMTSV40RSV,(GH)[4][6][12][19][26][27][28],[36][37][4][15][9][18][30][34][40][48][54][55][56][59](AFP)[16][17][20][21][25][39](Globin)[29],[36][66]GHAFP[2],[4][7][19][23][32][41][63][1][10][11][13][42][62],,PCR;Southern[43];NorthernRT-PCR;[47]480,,1985[64],,,1985[64],,(MT-1)DNA,(CyprinuscarpiohaematopterusTem.etSchl.)()(CarassiusauratusLinnaeus)(CarassiusauratusgibelioBloch)(MisgurnusangullicaudatusCan2tor),Southern,,50%,;50%,,,hGH,hGH,,,,,,,,,,,,[7][8][9],,,,,,2(CA)(gcGH)cDNA,CA5gcGH3,pCAz,gcGHcDNApCAz,GHpCAgcGHpCAgcGHc,,20%30%[66],GH,13(1994)MT-1(hGH)DNA,,,,,,,,DNA,AFPGHDNA(opAFP-GHc),DNA,(1995)GH,143,10%,,,4,,5-10m,;MTGH,,,[6][12][27][28],(bGH)(oGH)(1993)AFP,,[22]5[35][38][64];,,,,[4][39],DNA;(MAR)[44],,,10%,[3][19][46],,,,,,,,,,,,[67][1],1994.,40(1):96-99[2],1993.,14(3)35-41[3],1993.,3(9):23-26[4],1995.,2(2):23-33[5],1991.,15(3):286-28823[6],1991.,15(2):103-109[7],1986.,31(5):387-389[8],1989.(B),(2):147-155[9],1990.,14(2):176-178[10],1996.,20(3):242-247[11],1994.,4(3):20-22[12],1991.,15(4):372-374[13],1989.,(6):6-9[14],1991a.,1(1):13-16[15],1991b.,1(6):10-14[16],1991.,1(4):11-16[17],1992.,14(1):12-15[18],1992.,19(4):308-315[19],1994.,18(4):284-289[20],1992a.,8(2):192-196[21],1992b.,12(3):33-36[22],1993.,9(4):387-388[23],1989.,13(4):387-389[24],1996.,3(1):11-15[25],1989.,11(6):33-36[26],1990.,17(4):100-103[27],1992a.,8(2):140-144[28],1992b.,16(1):71-78[29],1990,(1):113-117[30]Agellon,L.B.etal.,1986,DNA,5:463-471.[31]Brem,G.etal.,1988.Aquaculture,68:209-219.[32]Buono,R.J.etal.,1992.Mol.Mar.Biol.Biotechnol.,1:346-349.[33]Benyumov.A.O.,etal.,1989.Genetica25:24-35.[34]Cavari,B.etal.,1988.Abst.3rdInternationalSymposiumofGenet2icsinAquaculture,Norway.[35]Chourrout,D.etal.,1986.Aquaculture,51:143-150.[36]Du,S.J.etal.,1992a.Biotech.,10:176-181.[37]Du,S.J.etal.,1992b.Mol.Mar.Biol.Biotechnol.,1:290-300.[38]Dunham,R.A.etal.,1987.Trans.Am.Fish.Sci.,116:87-91.[39]Fletcher,G.L.etal.,1988.Can.J.Fish.Aquat.Sci.,45:352-357.[40]Gonzalez2Villasenor,L.I.etal.,1988.Gene,65:239-246.[41]Inoue,K.S.etal.,1990.CellDiffer.Dev.,29:123-129.[42]Khoo,H.W.etal.,1992.Aquaculture,107(1):1-19.[43]Lavitrano,M.etal.,1989.Cell,57:717-723.[44]Phi2Van,L.etal.,1990.Mol.CellBio.,10(5):2302-2307.[45]Maclean,N.andTalwar,S.,1984.J.Embryol.Exp.Morphol.,82:187.[46]Mark,L.G.etal.,1992.Aquacultue,103:253-273.[47]McEvoy,T.etal.,1988.Auqaculture,68:27-37.[48]Nicoll,C.S.etal.,1987.Gen.Comp.Endocrinol.,68:387-399.[49]Ozata,K.etal.,1986.CellDiffer.,19:237-244.[50]Oshiro,T.,etal.,1989.InProceedingsFirstInternationalMarineBiotechnologyConference,AbstractNo.4-10[51]Palmiter,R.D.etal.,1982.Nature,300:611-615.[52]Powers,D.A.,1990.Mol.Repro.Dev.,25:3-13.[53]Rokkones,E.,etal.,1989.J.Comp.Physiol.158:751-758.[54]Sato,N.etal.,1988.Biochem.Biophys.Acta,949:35-42.[55]Sekine,S.etal.,1985.Proc.Natl.Acad.Sci.USA,82:4306-4310.[56]Song,S.etal.,1988.Eur.J.Biochem.,172:279-285.[57]Stuart,G.W.,etal.,1988.Development(Cambridge,UK)109,577-584[58]Tamiya,E.,etal.,1990.NucleicAcidsRes.18:1072[59]Watahiki,M.etal.,1988.Gen.Comp.Endocrinol.,70:401-406.[60]Yoon,S.J.,etal.,1988.J.Cell.Biochem.12B(Suppl.)190[61]Yoon,S.J.,etal.,1990.Aquaculture,.85:21-34.[62]Zelenin,A.V.etal.,1990.FEBSLETT.,287(1-2):118-124.[63]Zhao,X.etal.,1993.Mol.Mar.Biol.Biotechnol.,2(1):63-69.[64]Zhu,Z.etal.,1985.Z.Angew.Ichthyol.,1:31-34.[65]Zhu,Z.etal.,1986.KexueTogbao,31:988-990.[66]Zhu,Z.,1992.AgriculturalBiotechnology,106-116.ChinaScienceandTechnologyPress,Beijing.[67]Zhu,S.,1999.Biosecurityandanti2contamination,74-82.ChemicalIndu