高效液相色谱测定条件的耐用性试验研究

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(,150040)::,-,(HPLC):PH,,-:,PH(RSD310%),(RSD310%):,,:;;,-:R284:A:1002-2392(2010)03-0077-04:2009-11-30:2010-01-06:(ZHY06-Z53):(1963-),,,E-mail:[e-mail],,:pH,,,,,,,,,,’-,,2005[1],11.1Waters2695/2996;Waters1515/24871.2();,’-();();;();;(-)22.120,,,2g,,,(6090)30mL,,(250W,59kHz)30,,,(6090),,,10mL,,,10mL,,,0145m,2.2,’-,1mL0105mg,2.3::WatersSymmetryC18(416mm150mm,5m);:--(600400015);:110mLmin-1;:2521121210L,,1,,11(nm)(%)RSD(%)2664042934711771.631.680.10712674101414786351.691.610.10722684185934896531.651.510.10702704305395010651.781.440.10762724355985102011.791.420.10690.292754441585187151.981.370.10722784396665104411.941.410.10792804301154991411.771.420.10722824183514880031.761.460.10732834106994779191.661.620.1076,275nm,RSD=0129%,,,’-772010383Vol138,No13,2010ActaChineseMedicineandPharmacology©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.::WatersSymmetryC18(416mm150mm,5m);:--(600400015);:110mLmin-1;:275nm21121210L,,2,,22()(min)(%)RSD(%)204389664989651.581.5418.5580.1109254368034948451.681.4917.5500.11050.503.15304369024957431.661.4416.5000.1098354353305376751.981.3115.4250.1036,25,2035,RSD=3115%,35;2030,RSD=0151%,’-2.5::WatersSymmetryC18(416mm150mm,5m);:--(600400015);:25;:275nm21121210l,,3,,33(ml/min)(min)(%)RSD(%)1.23678294169761.561.3613.5750.11041.13972134539081.581.3515.4210.10961.04374804975571.691.2417.3140.10991.234.390.94843085429831.661.4619.2820.11260.85454866093461.441.9621.4780.1207,1mLmin-1,018112mLmin-1,RSD=4139%,018mLmin-1;019112mLmin-1,RSD=1123%,019112mLmin-1,’-2.6::Wa2tersSymmetryC18(416mm150mm,5m);:110mLmin-1;:25;:275nm21121210L,,4,,44(min)(%)RSD(%)14303145353451.641.6511.7680.110824328135292231.761.4415.9810.110434278495264201.891.3217.2280.10980.4644203105153841.991.4922.9860.109654085885014491.851.9627.9000.1105:1.--(640360015)21--(620380015)31--(600400015)41--(580420015)51--(560440015),600400015,(640360015)(560440015),RSD=0146%,,’-2.7PH::WatersSymmetryC18(416mm150mm,5m);:110mLmin-1;:25;:275nm21121210L,,5PH,,55PHPH(min)(%)RSD(%)12.124289465307431.561.2918.5160.107722.354330625317081.761.3818.1400.109632.464388755296811.881.3217.5150.10890.6842.604193095144942.041.4517.0760.109352.933985724984781.791.6316.8070.1092:1.--(600400110);2.--(6004000175);3.--(600400015);4.--(6004000125);5.--(6004000110),PH21122193,,’-,2.8::--(600400015);:25;:275nm;:110mLmin-121121210L,,6(ODSC18,416mm150mm,5m),,687ActaChineseMedicineandPharmacology2010383Vol138,No13,2010©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(%)RSD(%)14486894987851.571.5317.2380.108524378094798121.681.5017.7680.10691.0734467824975471.621.4717.6280.108744434864988021.731.4617.1670.1097:1HypersilC18();2GRACEGracesmartRPC18();3WatersSymmetryC18();4SHIMADZUC18(),,’-,2.92.9.110214gmL-1,’-110mL,210mL,310mL,410mL,510mL5ml,,10L,,,,3,,Y=1102107X-1143104,r=019996,77(n=3)(g)0.20481959880.40963985280.61446002200.9996Y=1.02107X-1.431040.81927968511.024010267658(n=3)(g)10.29833094220.33536958130.3734036260.9998Y=965375.47X+44240.1040.41043951850.447476029,,’-012048110240g2.9.2(010745mgmL-1)410mL,415mL,510mL,515mL,610mL10mL,,,,10L,,,8:0129810001447g33.1,210,(115),27510nm110012mLmin-12555%PH2146013,,:PH;,3.2,6617%75%8313%9117%100%,,’-,66%100%3.3,,:,275nm,,--(600:400:015),,’-,,,,,:[1].()[S].:,2005:116.[2].[S].2004::[H]CPH5-1.High-performanceLiquidChromatographyDeterminationofConditionsfortheDurabilityTestStudyTIANMing,LIWei,SUNHong-yu,FANZhuo-wen(HeilongjiangUniversityofChineseMedicine,Harbin150040,China)Abstract:ObjectiveThisstudyappearinginthefilmPurple,’-dimethyl-propyleneshikonindeterminationchroma2972010383Vol138,No13,2010ActaChineseMedicineandPharmacology©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(HPLC)conditionsforthedurability.MethodDeterminatetheagentsintargetcompositionof,’-dimethyl-acryl2amidecontentofshikoninundertheconditionofthedetectionofdifferentwavelength,flowrate,temperature,thepro2portionofmobilephase,thePHofmobilephase,column.ResultsInthetest,detectionatthemobilethanthematch,themobilephasePH,column,apparatusforthedeterminationofnosignificantimpact(RSD3.0%),flowrate,temperatureinfluence(RSD3.0%).ConclusionThroughthedurabilityteststudytodeterminethechromatographicdeterminationofappropriateconditions,adeterminationtoensuretheaccuracy,reproducibilityandcredibility.Keywords:DurabilityTest;HPLC;,’-dimethyl-propyleneshikonin1,2,1,13(1.,150040;2.,150001)::,,,:HPLC,ODS(200mm416mm,5m),-0102molL-1012%(pH=310),110mLmin-1,250nm,HPLC;,SPSS1610:,::;;HPLC;:R284:A:1002-2392(2010)03-0080-05:2009-12-05:2010-01-09:(:D200916);(:YJSCX2009-173HLJ):,,:3:,,,,,,,[1-2],,,[3],,,[4],MS,,,[5]11.1,,:PuerarialobataOhwi,ScutellariabaicalensisGeorgi,CoptischinensisFranch,GlycyrrhizauralensisFisch():08ActaChineseM