气相色谱技术在中药农药残留检测中的应用

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(,)[]:,:,;::,,[];;;[]R28411[]B[]100529903(2010)0220126209ApplicationofGasChromatographyforPesticideResiduesAnalysisinChineseMedicineHEPei2wen,ZHAOHai2yu,DUGang,WANGYi2tao3(InstituteofChineseMedicalSciences,UniversityofMacau,MacaoSAR,China)[Abstract]Objective:InordertoevaluatethesafetyofChinesemedicinecomprehensively,thedevelop2mentofpesticideresiduesdeterminationinChinesemedicinebygaschromatography(GC)wassummarizedinthispaper.Methods:Throughanalyticcomparison,thedevelopmentsofsamplepretreatmentsweresummarizedsystem2atically,includingsolid2phaseextraction(SPE),gelpermeationchromatography(GPC),supercriticalfluidextrac2tion(SFE),acceleratedsolventextraction(ACE),matrixsolidphasedispersion(MSPD),solid2phasemicro2ex2tration(SPME)andheadspaceanalysis(HS).Inaddition,thecomparisonofgaschromatographydetectorssuchasmassspectrometry(MS),electron2capturedetector(ECD),flamephotometricdetector(FPD)andnitrogenphosphorusdetector(NPD)werealsodiscussed.Results:Gaschromatographyisamaturetechnologytoaccom2plishpesticideresiduesdeterminationinChinesemedicine.Conclusion:GaschromatographyandrelevantnewtechniquesarewildlyusedinsafetyevaluationofChinesemedicineanditspreparations,whichwillbebenefitfortheinternationaldevelopmentandimportofChinesemedicine.[Keywords]Chinesemedicine;pesticideresidues;gaschromatography;samplepreparation[]2009212215[](FDCT049/2005/A2R1)[]3,Tel:[phone],Fax:[phone];E2mail:[e-mail],[14],,,2000[5],62116220102ChineseJournalofExperimentalTraditionalMedicalFormulaeVol.16,No.2Feb.,2010©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(GC),(HPLC)GC,,,,HPLC,,GCGC,80%[6],GC1GC1120()GPC,Florisil,GC2ECD200879GC2ECD200889GC2ECD20089FlorisilGC2ECD20081010,,GC2ECD2007119,,GC2ECD2007129,8,GC2ECD200713136GC2ECD200714GC2ECD2007158GC2ECD20071619SPEC18GC2ECD200717GC2ECD,FPD,MS20071895,,GC2ECD2006192GC2ECD20062015FlorisilGC2ECD2006219GC2ECD20062211,,9FlorisilGC2ECD20062319FlorisilGC2ECD200624105FlorisilGC2ECD200625FlorisilGC2MS/MS2006269GC2ECD200527321GC2ECD2005288GC2ECD2005298FlorisilGC2FPD20053018C18GC2PFPD20053113FlorisilGC2MS2005328GC2ECD20033310GC2ECD20033410,FlorisilGC2PCI2MS2003358,GC2ECD200236323GC2ECD200237(BHC)7GC2ECD2002389,,,GC2ECD200139912GC2ECD20004010116GC2ECD2000418GC2NPD1999428(),()GC2ECD19964313,,,,,FlorisilGC2FPD19964472116220102ChineseJournalofExperimentalTraditionalMedicalFormulaeVol.16,No.2Feb.,2010©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(SPE)SPE,,,[45],,SPE,2;();C18;(CN)[46]C18,89,[47]22,,15[48]C183,,C18SPE,[49],,[50]9,,,(ECD),7917%10113%,01002mgkg-1112(GPC)GPC(SEC),,,,,,;,,[5153]GPC,(SephadexLH220)(Bio2BeadsS2X),GPC[54],[55],GC2MS,90%,010020105gg-1[56],,113(SFE)SFE,(SCF),,SCF,[5758],SFE[59],SFEGC2ECD,Zhao[6061]SFE,,12Quan[62]9,SFE,SPEC18Zuin[63]SFE2GC2ECD/FPD,(PassifloraalataDryander)(PassifloraedulisSims.f.flvicarpaDeg.),6918%10711%,RSD1141417%[64265],CO2,8716%,8416%114(ASE)ASE1996Richter[66],,EPA()[67]Yi[68],ASE15,9512%[69]ASE,1664%124%,ASE282116220102ChineseJournalofExperimentalTraditionalMedicalFormulaeVol.16,No.2Feb.,2010©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[7071],ASEGC2MS/MS,80%106%,516%13%,1106000gL-1(R019967)[72]115(MSPD)MSPD(,,C18,C8),,,pH,,[7374]MSPD,,DDT75Zuin[75]MSPD(passionfruit),,,,116(SPME)11611(Direct2Immersion2SPME)DI2SPME,,1min,[76]Ro2drigues[77]DI2SPME,GC2MS(MikanialaevigataSchultzBip)(Maytenusilicifolia),60min,12ngg-1,012210ngg-1,90%108%Campillo[88]DI2SPME20min,GC2AED4210(),7315%10813%,11612(Head2Space2SPME)HS2SPME,,,,,[78]HS2SPME,,[79]Hwang[80]HS2SPME719,SPME,(ngg-1),,,HS2SPMEGC2MS,HS2SPME2GS2MS,13gkg-1[81]11613(Microwave2Assis2tedsolventExtraction2SPME)MAE2SPME:MAE(),,,Ho[82]MAE2SPME,0113ngg-1,(RSD)24%,MAEChen[93]MAE2HS2SPME515min,,110gL-1117(HS)11711(StaticHead2SpaceAnalysis)SHS,,[8486]92116220102ChineseJournalofExperimentalTraditionalMedicalFormulaeVol.16,No.2Feb.,2010©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[84]11712(DynamicHead2SpaceAnaly2sis)DHS,(),,,(ppt2ppb)Martin[87]2GCRoose[88]1h55,2211(ECD)ECD,2,(),(),,,,,ECDngg-1[2425,28,34],212(FPD)FPD,,,,,FPD1,,GC2FPD,0101101015gmL-14101218gL-1[30-31]213(NPD)NPD,(FID),(),,NPD510-14gPsec-1FID110-12gPsec-1500[89],NPD214(MS)3,,,,,(SIM),,,,,[31]213,81%118%88%119%,410%919%517%915%,,(TIC),;,,,MartinezVidal[90]15130,70%120%,RSD15%,312916gkg-1;;,Stanis¤aw[91]136122,(,,),,(QqQ),QqQ;QqQ,PlazaBolanos[92],415h03116220102ChineseJournalofExperimentalTraditionalMedicalFormulaeVol.16,No.2Feb.,2010©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(IonTap),,QqQ,[93]3,,,,,,,,[][1],,.[J].,2007,23(3):299.[2],,.18[J].,2006,31(16):1321.[3],,.16[J].,2006,41(9):1497.[4],,.-[J].,2007,32(6):550.[5].[S]..:,2000:XIQ.[6].[J].,2006,12(16):127.[7],,.()[J].,2008,19(2):126.[8],,,.[J].,2008,17(6)