激光共聚焦生物芯片分析仪的二维扫描研究Ξ

X(310027)CCDPMT,XY,ResearchoftheTwo-dimensionalScanningonLaserConfocalBiochipAnalyzerLiYingshengNiXuxiangLuZukangWangLiqiang(CNERCforOpticalInstrument,ZhejiangUniversity,Hangzhou310027,China)AbstractThispaperfirstlyinterpretsthecomponentsandfeaturesoflaserconfocalbiochipanalyzerandthedif2ferencesbetweenCCDandPMTanalyzer,thendiscussesthetechnologyofthemechanicalXYdimensionalscanningonanalyzeranditsdeficiency.Bycomparingtheformer,thecharactersoftheopticmechanicaltwo2di2mensionalscanning,themainparametersandthepreliminaryresultsinexperimentaremainlyintroduced.KeywordsBiochipLaserconfocalTwo2dimensionalscanning1,,,,[1],,,,,(PMT)CCD[23],PMT,,CCD,PMTCCD,PMT,,,,;CCD,CCD16mm12mm(10Lm)[3],22mm70mm,,,PMT,,,2211[4],,1,,,PMT24420038X(30227002),PMT,,PMT,,PMT,,,,,0.3,,,XY1212XY[5]2()XY,22,,X,,Y,,,,,,XY,,,,,,2NA,,31,X,Y1,,,,,1,XY,3()3,[6]f2H[7]X,4f2HHGXf2H,x=f2H(H,f)[7],X,HY,,:(1)I,X,X,7424(2)f2HF=2.4(NA=0.2),,,324()A1A2B1B2C1C2DEF()GHf2HIJKLM(3),X,,100Hz,20Hz80Hz4,40Lm,(22mm70mm)70s(,50Hz),(4),,f2H,,,(5)(Lrad)(Lrad),X,,,,,,4,,4PMT,,70mm22mm,Y,=m1.8,Ysr1mmö360,fm=Lts1sr1=m(L=70mm)136.6kHz48.5kHz73.3kHz146.7kHz40Lm30Lm20Lm10Lmts354770140709314028050Hz50Hz50Hz50Hzfm400Hz300Hz200Hz100Hz15mins,,2mins5mins[8],5,532nm,40Lm,355,GenePix4000B,(0.3mm),,842245f2H,,,,,,1,,,.DNA.,2000,20(2):6168.2,,,..,2002,24(45):5965.3.,2002,(3).http:öö(DNA).,2001,(3).http:öö:284292.6Model6450GalvanometerOpticalScannerInsructionManual.CambridgeTechnology,Inc.,November21,1996.7..:,1997.352357.8Y.F.Leung.Lookingforamicroarrayscanner.Depart2mentofOphthalmologyandVisualSciences,ChineseU2niversityofHongKong.http:öö(245)DSPR0R1R4R5IIRy(n)x(n)668IIR5,DSPBLUESDISTMETALOVER2DRIVE,,1,.[M].:,1999.2.[M].:,2001.3,.[J].,2001,(4).4.LMS[J].,1994,5(4).5,.DSP[J].(),2002,30(2).6SimonHaykin.Adaptivefiltertheory.4thedition[M],NJ:PrenticeHall,2002.7DavidRA.IIRadaptivealgorithmsbasedongradientsearchtechniques[D].StanfordUniv.,Standford,Calif.,Aug.1981.9424