几种细胞脂肪变性模型的建立与比较分析_罗燕

35820178CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol．35No．8Aug．20172074DOI10．13193/j．issn．1673-7717．2017．08．040111211．6505002．650500。。L－02、HepG2、3T3－L1、BRL、Changliver、、L8824。、、24～48h。TGTC。R285．1A1673-7717201708-2074-04EstablishmentandComparativeAnalysisofSeveralKindsofCellFattyDegenerationModelLUOYan1HEXingping1LIXue1CHENChaoyin2ZHAOShenglan11．FacultyofTraditionalChineseMedicineYunnanUniversityofTraditionalChineseMedicineKunming650500YunnanChina2．FacultyofLifeKunmingUniversityofScienceandTechnologyKunming650500YunnanChinaAbstractHyperlipidemiaandobesitybecomeseriouspublichealthproblemsattractingwidespreadattention．Cellmodelisoneoftheimportantmeansofstudylipid－loweringdiet．ThispapercomparedandanalyzedthemethodsandeffectofdifferenttypesofcelllinestosetuphighfatcellmodelincludinghumanliverL－02cellshumanHepG2livercells3T3－L1preadipocytesinmiceratliverBRLcellshumanliverChanglivercellscowslivercellsandgrasscarplivercellsL8824．Mostofinducinghighfatcellswereintheendoflogarithmicphaseinducedbyfattyacidfate-mulsionandhighconcentrationsoffetalbovineserumtheinductiontimeabout24to48hours．DifferentcelllinesandinductionmethodleadtolargedifferentascendingrangeintriglyceridesTGandtotalcholesterolTC．Keywordslipid－loweringdietinductionfattydegenerationcellmodel2017－04－07214660372011BAD46B00ZD2014009－U01201502421992－。1962－。[e-mail]。12。。3－5、6。。1L－02L－027、。L－0210%1640、、+、37℃、5%CO2TG32%～428%O8－13。1．1、80%10%16400．5mol·L－148hTG428%14。1．248h10%1640470235820178CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol．35No．8Aug．2017207550%48hTG143%14TC79%10。1．3∶=2∶11%BSA16401mmol∶=2∶1164024hTG374%1116。1．448hL－02164020%1．0mL·L－1164048h。TG40%1219～150%18ALT87%AST62%。2HepG2HepG2、20－21。10%DMEM、+、TG105%～1100%O22－25。2．110%FBSDMEM10%FBSDMEM0．2mmol·L－124h。TG105%22～338%23。2．2∶=2∶170%～80%10%DMEM1mmol·L－1∶=2∶110%DMEM24hTG341%26MDATC170%4。2．3+5mmol·L－1、5000nmol·L－1、5%BSADMEM、DMEM100nmol·L－1、1mmol·L－1、1%BSA。HepG280%～90%DMEM48h。TG187%2427。2．4HepG210%、100U·mL－110%DMEM1%20%2024h。TG1100%2528。33T3－L13T3－L117～19dSwiss3T33T3－L13T3－L1。3T3－L1、29－31242dI0．5mmol·L－1IBMX、1mmol·L－1DEX、10μg·mL－102dIIDMEM10mg·L－1。3T3－L1TG2932－35。3T3－L129。4BRLBRL36－37。BRL90%1%BSA12h0．1mmol·L－124hTG41%38。5ChangliverChangliverL02NAFLD39。Changliver104/9610%FBSDMEM200μL/0．2mmol·L－1200μL/24hTG105%39。640。5mmol·L－1DL－48hALT、AST158%、66%TG489%40。570235820178CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol．35No．8Aug．201720761L0220mg·L－148hTG14。50%48hTGTC9－1014。1mM∶=2∶124hTG411。1．0mL·L－120%48hTG18。HepG20．2mM·L－124hTG22－23。1mM·L－1∶=2∶124hTGTC426。+48hTG2427。1%24hTG、2528。3T3－L1I0．5mM·L－1IBMX、1mM·L－1DEX、10mg·L－12dIIDMEM10mg·L－1。、。42。BRL0．1mM·L－124hTG38。Changliver0．2mM·L－124hTG39。5mM·L－1DL－48hTG40。L88241mL·L－148hTG。41。7L8824L88242475%～80%10%FBSM1991mL·L－1M19948hTG39%ALT、AST41。1TGTC1～2。1TG22TCOTGTGTCTGTC、、。1Gonzalez－CastejonMRodriguez－CasadoA．Dietaryphyto-chemicalsandtheirpotentialeffectsonobesityareviewJ．PharmacolRes2011645438－455．2SzatkowskiCValletJDormishianMetal．Prokineticinre-ceptor1asanovelsuppressorofpreadipocyteproliferationanddifferentiationtocontrolobesityJ．PLoSOne2013812e81175．3TorresDMWilliamsCDHarrisonSA．Featuresdiagnosis670235820178CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEVol．35No．8Aug．20172077andtreatmentofnonalcoholicfattyliverdiseaseJ．ClinGastro-enterolHepatol2012108837－858．4HwangYPKimHGChoiJHetal．S－allylcysteineatten-uatesfreefattyacid－inducedlipogenesisinhumanHepG2cellsthroughactivationoftheAMP－activatedproteinkinase－de-pendentpathwayJ．JNutrBiochem20132481469－1478．5SongCYShiJZengXetal．Sophocarpinealleviateshepato-cytesteatosisthroughactivatingAMPKsignalingpathwayJ．ToxicolInVitro20132731065－1071．6SternJSPeersonJMishraATetal．EfficacyandtolerabilityofanovelherbalformulationforweightmanagementJ．ObesitySilverSpring2013215921－927．7WangMZhaoRWangWetal．LipidregulationeffectsofPolygoniMultifloriRadixitsprocessedproductsanditsmajorsubstancesonsteatosishumanlivercelllineL02J．JEthno-pharmacol20121391287－293．8．、PGC－1αD．2014．9．L－02J．2013325487－493．10．L－02J．20111336－11．11．L－02J．2014346837－842．12．L－02TGJ．2011313247－254．13．L－02J．2015324469－473．14．J．2010184297－299．15．PNPLA3CpGJ．2015154626635－639．16．L02D．2014．17．ERp57J．2014482119－123．18．J．200715353674－3677．19．“”J．2011312129－135．20．、J．2014344401－407．21FerlazzoNCirmiSRussoMetal．NF－kappaBmediatestheantiproliferativeandproapoptoticeffectsofbergamotjuiceinHepG2cellsJ．LifeSci2016146181－91．22．J．2015153349－350．23．HepG2J．20152816－8．24．J．201122102384－2386．25．J．2010156446－448．26．J．2009386626－629．27．J．201127524－26．28．D．2010．29．3T3－L1PUFAD．2014．30RohCJungU．Screeningofcrudeplantextractswithanti－o-besityactivityJ．IntJMolSci20121321710－1719．31LeeKHSongJLParkESetal．Anti－ObesityEffectsofStarterFermentedKimchion3T3－L1AdipocytesJ．PrevNutrFoodSci2015204298－302．32．D．2014．33．D．2013．34．D．2014．35．AMPKmiRNAD．2014．36．BRL－3AJ．201535101320－1324．37．BRL－3AJ．．．201628146－50．38．J．20116111349－1351．39．ChangliverJ．201443334494－4497．40．CYP450D．2013．41．J．201522124－32．42．3T3－L1J．201313121542－15441662．7702