小鼠海马CA1和CA3神经元基本电生理特性的比较分析

CA1CA31121[e-mail]1CA3。CA1CA3。CA1CA3。CA1CA3、P＜0.05。CA1CA3。Ｒ338．8A1000－1492201408－1072－05CA1CA31－2。CA1。CA3CA3CA1。CA1CA3。CA1CA3。11．1C57/BL61014d30g。1．21．2．1LeicaVT1200LeicaBiosystemsNusslochGmbHP-98HEKAOlympus。1．2．2Sigma。mmol/LSucrose213、Glucose10、KCl3、NaH2PO41、CaCl20．5、MgCl25、NaHCO326NaOHHClpH7．30～7．40310mOsm。mmol/LGlucose10、NaCl125、KCl5、NaH2PO41．2、CaCl22．6、MgCl21．3、NaHCO326pH7．30～7．40。mmol/LKCl145、NaCl5、HEPES10、EGTA5、MgATP4、Na2GTP0．3pH7．30～7．40310mOsm。1．314d1%3。0℃95%O2+5%CO230min0℃、α-0℃400μm30min1～2h4。1．4U。U1～2ml/min2mm5。·2701·ActaUniversitatisMedicinalisAnhui2014Aug4981．5CA1CA3。－60mV-。IGOＲ。1．6GraphpadPrism5．0。珋x±stTwo-WayANOVA。22．110CA1CA340CA1CA3CA1CA31。1×40ACA1BCA32．22．2．1－60mVCA1CA32。CA1CA3531.10±88.03PAn=9305.00±90.03PAn=60.74±0.15Hzn=91.08±0.12Hzn=6t=1.72P=0.10t=1.60P=0.13。－60mV－60mV20mV80mV200ms3。-I-V=/PA/PF6。4CA1CA3－50mVCA1CA3F=4.41P=0.05P＜0.05CA1CA3－50mV－40mVF=3.04P=0.09P＜0.05。2CA1CA3ACA1BCA33·3701·ActaUniversitatisMedicinalisAnhui2014Aug4984CA1CA3I－VAI－VBI－V2．2．2CA1CA35。CA1CA3CA1CA393.19±4.08mVn=12101.10±4.42mVn=100.96±0.33Hzn=120.95±0.34Hzn=10t=1.30P=0.20t=0.02P=0.97。CA1CA3－60.07±0.43mVn=12－60.44±1.39mVn=10CA1CA34601.00±1785.00msn=123884.00±1654.00msn=10t=0.27P=0.78t=0.29P=0.77。－60mV50PA－650PA50PACA1CA36A、B。－300PA－50PAP＜0.05CA1CA3F=3.57P=0.066C。5CA1CA3ACA1BCA36CA1CA3ACA1BCA3CCA1n=19CA3n=133·4701·ActaUniversitatisMedicinalisAnhui2014Aug4987。、、、8。9、、。10。pH、、11。CA1CA3。CA1CA3CA3CA1。CA1CA312。CA1CA3PA1．0Hz。CA1CA3－60mV100mV1～10Hz－50mV13。CA1CA3CA1CA3。CA1CA3CA1CA3。1．N--D-J．200484634－6．2NewrzellaDPahlavanPSKrugerCetal．ThefunctiongenomeofCA1andCA3neuronsundernativeconditionsandinresponsetoischemiaJ．BMCGenomic20078370．3．NＲ1LTPJ．201247147－53．4MohajeraniMHSivakumaranSZacchiPetal．Correlatednet-workactivityenhancessynapticefficacyviaBDNFandtheEＲKpathwayatimmatureCA1-CA3connectionsinthehippocampusJ．PNAS20071043213176－81．5．CA1J．200425131153－6．6．J．2007592278－84．7KayAＲKrupaDJ．AcuteisolationofneuronsfromthematuremammaliancentralnervoussystemJ．CurrProtocNeurosci2001Chapter6．8．J．2001535405－8．9．P2J．2013483207－10．10．J．2009295283－5．11．J．2012333449－52．12MizusekiKＲoyerSDibaKetal．Activitydynamicsandbe-havioralcorrelatesofCA3andCA1hippocampalpyramidalneu-ronsJ．Hippocampus20122281659－80．13AndersenPMorrisＲAmaralDetal．TheHippocampusBookM．NewYorkOxfordUniversityPress2007139－60．ComparativeanalysisofbasicelectrophysiologicalcharacteristicsofhippocampalCA1andCA3neuronsinmiceZhangYusong1ZhuYan12GuoChengbin1etal1DeptofPhysiologyCollegeofBasicMedicineAnhuiMedicalUniversityHefei2300322DeptofPhysiologyBengbuMedicalCollegeBengbu233000AbstractObjectiveAcutemousehippocampalsliceswerepreparedforpatchclamprecordinginordertoobserve·5701·ActaUniversitatisMedicinalisAnhui2014Aug498thebasicelectrophysiologicalpropertiesofhippocampalCA1andCA3neurons．MethodsThestructurepropertiesandbasicelectrophysiologicalpropertiesofmousehippocampalsliceswereobservedandrecordedbyinfrareddiffer-entialinterferencecontrasttechniqueandpatchclamprespectively．ＲesultsThehippocampalslicestructurewasobservedclearlywithhealthyglossandfewdeadneuronsandthehippocampalneuronsweresealedeasilywithpatchclamptechnique．ThespontaneousdischargecurrentandactionpotentialbetweenCA1andCA3neuronsdidnotshowsignificantdifferencesinamplitudefrequencyinterspike-intervalsandrestingmembranepotentials．ThecurrentdensityofsodiumandpotassiumionwheninjectingpulsestimulationsandtheactionpotentialnumberswheninjectingstepcurrentsshowedsignificantdifferencesinCA1andCA3neuronsP＜0.05．ConclusionSomebasicelectrophysiologicalpropertiesofhippocampalCA1andCA3neuronsprovidecertaintheoreticalbasisformemoryandnervediseasesresearch．KeywordspatchclampmousebrainsliceshippocampalneuronselectrophysiologyMSCsMSCs。8。、。MSCs。2、4、8、12414SNK。8Sharpey12Sharpey。4P＜0.05。MSCs。Ｒ686A1000－1492201408－1076－04。[e-mail]1－3。、、。40.7%～10.0%。、、、、、5。mes-enchymalstemcellsMSCs。11．1MSCs、5ml5ml37℃、5%CO248～72h72h190%。3MSCsCD11b、CD34、CD29、CD90。1．28。2.5±0.3mm·6701·ActaUniversitatisMedicinalisAnhui2014Aug498