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37420098JOURNALOFZHEJIANGUNIVERSITYOFTECHNOLOGYVol.37No.4Aug.2009:2008209201:(1962),,,,,.,,(,310032):,,,.,,,,.:;;:X592:A:100624303(2009)0420386206DevelopmentofenzymeinhibitionforrapiddetectionoforganophosphoruspesticideresiduesLINChun2mian,HUXiao2yan,ZHANGAn2ping(ResearchCenterofEnvironmentalScience,ZhejiangUniversityofTechnology,Hangzhou310032,China)Abstract:Asakindofefficientandbroadspectruminsecticides,organophosphoruspesticides(Ops)havebeenwidelyusedinagriculture,homeandstorage.Whileusingtoomuchwouldcausemuchpotentialharm,suchasenvironmentalpollution,pharmcologicalcontanminationandthetoxicosisofpeopleandanimals,especiallythetoxicosiscausedbyOpsresiduesinvegetableswasrathersevere.Therefore,itisimportanttoestablishaconveninent,rapidandaccuratemethodfordeterminationofOpsresidues.Basedonenzymeinhibitionprinciple,thedevelopmentofrapiddetectionofOpsresidueswithplant2esteraseandacetylcholinesterasissummarized.Keywords:organophosphoruspesticides;plant2esterase;acetylcholinesteras0,,,[1].:,,;;;.,,,[2].,.,300,70%.,,,,.,,..11.12030,Schrader,.100,50.30,38%,75%.,,.:(1).,;(2),,();(3).,.,;(4).,;(5),[3].1.2,,[4].,,.,,.4d,[5].2:.,,,,,;,,,;,,,..2.1,1953stedman,[6].,,.,,[628].:[9].,,,,23d[10].,,.,,,,..2.27834,:,.,5,522222,52222,410nm,,.,B,,,,,,[11].,,,2004,,85%,3%,[11].pH,,pH6.571%,pH6.073%[12].0.030.40mg/kg,0.040.50mg/kg[13].,,,,.2.31951,.,.2.3.1,,().,.,,()..,0.33.5mg/kg,15min,,[14].,pH,,[15].,Hwa2YoungNoYoungAhKim,HybondN+,88337,10-6102g/mL10-6100g/mL,:,[16].2.3.2,,.,,.,.,(),397%,101%99%[17].IngridWaltpisi,96,2.6mg/L0.04mg/L[18].2.3.3,.(1):.,O2H2O2.Andreescu,,Ag/Al,,5.210-3mg/L0.5610-3mg/L[19].(2):.,pH,pH.,.,,[20].,pHpH,AChE,[21].Pardo2YissarAChECdS.AChE,AChE,,.CdS[22].(3):(ChE),ChE,,.AbadAChE.AChE,[23].Makower,.BChE,,BChE[24].ChE,.,Knosche(benzoylecgonine),AChE,,[25].Nunes(ChE),,510-5g/g,110-43.5g/g[26].HolgerSchulzeAchE2N2,,2h,84%,2g/kg,[27].2.4,,,.,,,9834,:-4.,,.1987,,,,0.510mg/kg.(),0.410mg/L0.1810mg/kg,510min,0.20.3[28229].,,.,,,,,.,,.,B.,40min,,,[30].,[31],,,3,10-410-7.2,62,2,62,(),,0.00150.3300mg/L.(2,62B),2,62,,2,62B[[32233].:205d39.6%,45d75.6%,,-1968d,97.5%,[34].,,,,[35].,,,0.010.1mg/kg[36].2PVA/SiO2/,,2,.[37].,.3,,.,.,,,,.,,,.,,,.,,.:[1],.[J].,2001,27(3):31235.[2],,.09337[J].,2004,22(4):58259.[3],.[M].:,1997:2792280.[4],,.[J].,2001,22(1):28230.[5],.[J].,2002,16(2):1432146.[6],.[J].,1997,22(3):21223.[7].5AChE2[J].,2003,12(8):529.[8],,.(AChE)[J].,2003,12(6):72279.[9],.[J].,2002,6(2):57262.[10],.[J].,2003,4(1):972100.[11].[J].,2003,12(8):1182120.[12],.[J].,2003,24(1):21223.[13].[J].,2006,5(1):1412143.[14],,,.[J].,2003,15(1):39241.[15].[J].,2004,12(4):47249.[16]NOHY,KIMYA.Cholinesterase2baseddipstickassayforthedetectionoforganophosphatesandcarbamatepesticides[J].AnalyticaChimicaActa,2007,594(1):37243.[17],.[J].,2005,12(6):7112713.[18]WALZI,SCHWACKWF.Cutinaseinhibitionbymeansofinsecticidalorganophosphatesandcar2bamates[J].Agricul2turalandFoodChemistry,2007,55(20):817728186.[19]ANDREESCUS,AVRAMESCUA,BALAC.De2tectionoforganophosphorusinsecticideswithim2mobilizedacetylcho2linesterase2comparativestudyoftwoenzymesensors[J].A2nalBioanalChem,2002,374(1):39245.[20]ANDREOUVG,CLONISYD.Aportablefiber2opticpes2ticidebiosensorbasedonimmobilizedcholinesteraseandsol2gelentrappedbromcresolpurpleforin2fielduse[J].BiosensBioelectron,2002,17(3):61269.[21]WHITEBJ,HARMONHJ.Interactionofmono2sulfonatetetraphenylporphyrin,acompetitiveinhibitor,withacetyl2cholin2esterase[J].BiosensBioelectron,2002,17(6):4632469.[22]PARDOYV,KATZE,WASSERMANJ.Acetyl2cholineesterase2labeledCdSnanoparticlesonelectrodes:photoelec2tro2chemicalsensingoftheenzymeinhibitors[J].AmChemSoc,2003,125(3):6222623.[23]ABADJM,PARIENTEF,HERNANDEZL,etal.Deter2minationoforganophosphorusandcarbamatepesticidesusingpiezoelectricbiosensors[J].AnalChem,1998,70(14):284822855.[24]MAKOWERA,HALAMEKJ,SKLADALP.Newprinci2pleofdirectreal2timemonitoringoftheinter2actionofcholin2esteraseanditsinhibitorsbypie2zolectricbiosensor[J].Bio2sensBioelectron,2003,18(11):132921337.[25]KNOSCHEK,HALAMEKJ,MAKOWERA,etal.Mo2lecularrecognitionofcocainebyacetylcholine2esterasesforaffinitypurificationandbio2sensing[J].BiosensBioelectron,2004,20(2):1532160.[26]NUNESGS,BARCELOD.Electrochemicalbio2sensorsforpesticidedeterminationinfoodsample[J].PesticideAnaly2sis,1998,26:1562159.[27]HOLGERS,ROLFDS.Rapiddetectionofneuro2toxicin2secticidesinfoodusingdisposableacetyl2cholinesterase2bio2sensorsandsimplesolventextr2action[J].AnalBioanalChem,2002,372:2682272.[28],,,.[J].,2000,19(2):1892189.[29],,,.[J].,2001,22(4):29231.[30],,.[J].,1999,11(4):29230.[31],,,.[J].,2001,40(9):19220.[32],,,.[J].,2002,21(2):1892193.[33],.[J].,2003,14(3):40242.[34],,,.[J].,2003,24(4):6822685[35],.[J].,2003,24(5):27232.[36],.[J].,2004,24(3):5582560.[37],.[J].,2007,35(2):89292.(:)1934,:
本文标题:酶抑制法快速检测有机磷农药残留的研究进展
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