年产60吨硫酸庆大霉素发酵车间设计概论

内蒙古科技大学本科生毕业设计说明书题目：年产60吨硫酸庆大霉素发酵工段工艺设计学生姓名：张伟男学号：0966121207专业：生物工程班级：09-2班指导教师：崔向军副教授内蒙古科技大学本科生毕业设计说明书I年产60吨硫酸庆大霉素发酵车间工程设计摘要本次设计任务为年产60吨庆大霉素发酵车间工程设计。本设计采用三级发酵。共有八个发酵罐，两个二级种子罐，两个一级种子罐。采用的生产工艺如下：生产菌种为小单胞菌，经小、中罐种子扩大培养后接到发酵罐中。工艺过程为小—中—大罐三级发酵，小、中罐一次投入。大罐考虑底物抑制及产物营养要求，采用中间补料，包括全料、稀料、氨水、氢氧化钠、消沫油。发酵罐主要设计参数为：公称直径4500mm，罐高11500mm，搅拌器直径为1410mm，筒体壁厚16mm；二级种子罐的主要设计参数：公称直径2400mm，罐高6000mm，搅拌器直径750mm，筒体壁厚9mm；一级种子罐的主要参数：公称直径1400mm，罐高3500mm，搅拌器直径437.5mm，筒体壁厚7mm。设计说明书完成后根据说明书绘制了发酵罐和种子罐的装配图、工艺流程图及厂房布局图。厂房布局结合实际情况，高15米、长54米、宽17.4米。此外还对环境污染和节省能源方面的设备做了合理的布局，主要工段和设备大部分在厂房内，部分设备在厂房周围。关键词：硫酸庆大霉素；发酵工艺；罐体参数；厂房布局内蒙古科技大学本科生毕业设计说明书IIEngineeringdesignoftheGentamicinSulphatefermentationworkshopwiththecapacityof60tonsannuallyAbstractThedesigntaskwasengineeringdesignoftheGentamicinSulphatefermentationworkshopwiththecapacityof60tonsannually.Threelevelsfermentationwasusedinthisdesign.Therewereeightfermentors,twosecond-gradeseedpotsandtwoone-gradeseedpots.Theproductionprocesswasfollowed:producingstrainsisMicromonospora.Aftercultureexpansionoftheseedsbythesmallandmediumcans,theywerevaccinatedtothefermentors.Threelevelsfermentationwasusedinthetechnologicalprocessforthesmall—medium—bigtank.Smallandmediumtankwasputinonetime.Intermediatefedwasusedwithinthebigtankconsideringofrequestofsubstrateinhibitionandproductnutrition.Intermediatefedincludefullmaterial,thinnermaterial,ammonia,NaOH,crawlagentfrothoil.Themaindesignparametersoffermentersis:thenominaldiameteris4500mm;theheightIs11500mm;thediameterofthemixeris1410mm;thethicknessofcylinderwallis16mm.Themaindesignparametersofsecond-gradeseedpotis:thenominaldiameteris2400mm;theheightis6000mm,thediameterofthemixeris750mm;thethicknessofcylinderwallis9mm.Themaindesignparametersofone-gradeseedpotis:thenominaldiameteris1400mm;theheightis3500mm;thediameterofthemixeris560mm;thethicknessofcylinderwallis7mm.Afterthedesignmanualwascompleted,thefermentersandtheseedfermentorsassembledrawing;workingprocessdiagramandpowerhouselayoutdrawingweredrawnaccordingtothedesignmanual.Theworkshoplayoutcombinedwithpracticewasdesigned.Theworkshopis15metershigh,54meterslong,17.4meterswide.Inaddition,thereasonablelayoutoftheequipmentonenergy-savingmeasuresandenvironmentalpollutionwasmade.Mainworkshopsectionandequipmentsweremostlyinworkshop.Partialequipmentsarearoundworkshop.Keywords:GentamicinSulphate;fermentationtechnology;parameteroftank;layoutofpowerhouse内蒙古科技大学本科生毕业设计说明书III目录摘要...........................................................................................................................................IAbstract......................................................................................................................................II绪论.............................................................................................................................................1第一章总论...............................................................................................................................21.1生产设计任务................................................................................................................21.1.1设计指导思想......................................................................................................21.1.2设计要求..............................................................................................................21.1.3设计原则..............................................................................................................21.1.4设计范围..............................................................................................................31.1.5设计目标..............................................................................................................31.2历史介绍........................................................................................................................31.3产品介绍........................................................................................................................31.3.1产品简介..............................................................................................................31.3.2产品名称及主要特征..........................................................................................41.4工艺设计指标................................................................................................................61.4.1设计基本参数......................................................................................................61.4.2技术规格及质量标准........................................................................................11第二章工艺流程概况.............................................................................................................122.1菌种筛选......................................................................................................................122.2合成途径......................................................................................................................132.3工艺流程......................................................................................................................13