15ml超滤离心管使用说明书

Amicon®Ultra-15CentrifugalFilterDevicesforvolumesupto15mLUserGuideAmiconUltra-1510KdeviceforinvitrodiagnosticuseAmiconUltra-153K,30K,50K,and100Kdevicesforresearchuseonly;notforuseindiagnosticprocedures������������������������������������PR02842_RevB.indd2910/30/200911:42:29AMNoticeTheinformationinthisdocumentissubjecttochangewithoutnoticeandshouldnotbeconstruedasacommitmentbyMilliporeCorporationoranaffiliatedcorporation.NeitherMilliporeCorporationnoranyofitsaffiliatedcorporationsassumesresponsibilityforanyerrorsthatmayappearinthisdocument.Thismanualisbelievedtobecompleteandaccurateatthetimeofpublication.InnoeventshallMilliporeCorporationbeliableforincidentalorconsequentialdamagesinconnectionwithorarisingfromtheuseofthismanual.©2009MILLIPORECORPORATION.ALLRIGHTSRESERVED.PR02842Rev.B,10/09Millipore,Amicon,Centricon,Milli-Q,MultiScreen,andUltracelareregisteredtrademarksofMilliporeCorporation.TheMmarkisatrademarkofMilliporeCorporation.AlconoxandTerg-A-ZymeareregisteredtrademarksofAlconox,Inc.LubrolisaregisteredtrademarkofImperialChemicalIndustriesPLC.NonidetisatrademarkofRoyalDutch/ShellGroup.TritonisaregisteredtrademarkofUnionCarbideCorporation.TweenisaregisteredtrademarkofICIAmericas,Inc.PR02842_RevB.indd3010/30/200911:42:29AMContentsIntroduction..........................................................................................................................................................1Applications.........................................................................................................................................................2MaterialsSupplied...............................................................................................................................................3RequiredEquipment............................................................................................................................................4Suitability.............................................................................................................................................................4DeviceStorage....................................................................................................................................................4Specifications......................................................................................................................................................5ChemicalCompatibility........................................................................................................................................7HowtoUseAmiconUltra-15CentrifugalFilterDevices.......................................................................................9HowtoQuantitateRecoveries...........................................................................................................................10Performance-ProteinConcentration................................................................................................................13FlowRate...................................................................................................................................................13ProteinRetentionandConcentrateRecovery............................................................................................18MaximizingSampleRecovery...........................................................................................................................21DesaltingorDiafiltration.....................................................................................................................................22CentrifugalProductOrderingInformation..........................................................................................................23TechnicalAssistance.........................................................................................................................................24InVitroDiagnosticProductLabeling..................................................................................................................25StandardWarranty.............................................................................................................................................26PR02842_RevB.indd3110/30/200911:42:29AMIntroductionMillipore’sAmicon®Ultra-15centrifugalfilterdevicesprovidefastultrafiltration,withthecapabilityforhighconcentrationfactorsandeasyconcentraterecoveryfromdiluteandcomplexsamplematrices.Theverticaldesignandavailablemembranesurfaceareaprovidefastsampleprocessing,highsamplerecovery(typicallygreaterthan90%ofdilutestartingsolution),andthecapabilityfor80-foldconcentration.Typicalprocessingtimeis15to60minutesdependingonNominalMolecularWeightLimit(NMWL).Solutepolarizationandsubsequentfoulingofthemembraneareminimizedbytheverticaldesign,andaphysicaldeadstopinthefilterdevicepreventsspinningtodrynessandpotentialsampleloss.Theconcentrateiscollectedfromthefilterdevicesamplereservoirusingapipettor,whiletheultrafiltrateiscollectedintheprovidedcentrifugetube.Thede