园艺植物生物技术之快速繁殖

123Whileapplyingthetechniqueofshoot-tipcultureforraisingvirus-freeindividualsofanorchid,Morel(1960)alsorealizedthepotentialofthismethodfortherapidpropagationoftheseplants.Thetechniqueallowedtheproductionofanestimated4milliongeneticallyidenticalplantsfromasinglebudinaperiodof1year.“”“”“”“”1ABCD……Invitro=Invitro=invivoinvivoBranchingAdventitiousGrowthCallusAgricultureincludes:–Plantselection(usefulness)–Plantbreeding(forspecifictraits)–Plantpropagation(selectedplantschosentomaintaintheirtraits)–Cropproduction(growingundercontrolledconditionsformaximumyield)–Foodtechnology(preservingplantproductsasasourceoffood)llllu••u••••§§§§§§u•••§§§§“”§•protocorm•••—§•••••germinationonbarkasymbioticgerminationSeedgermination1.2.,3.15-204.5.6.7.8.,9.Nodeculturecultureinsoilcultureinvitrol••§§§§§•35,5§2n=1633.9%9.2%15.4%9.2%24.6%16.9%7.7%§§§§§—“”invivostage4:acclimatizationinvivostage0:preparationofthemotherplantinvitrostage1:asepticinitiationstage2:multiplicationstage3a:elongationstage3b:rootinductionandpre-acclimatization••••••pJuvenile)maturerejvenationdetermination)pppØ••••Ø••••Ø§—§—p§§01020304050607080510152025§§2626940167%03.871.3“”§140664404310814210%77252.50§••§“”§01020304050607080§Itisacompromisebetweenanadequatesterilizationprocedureandagoodrateofsurvivalof“noncontaminated”explants.§§§§§•••••••§§§§§§§§§§§§§••••nn•••••••••••••§HMHM+2,4-D0.1+BA1CytokininconcentrationCytokininconcentrationAuxinconcentrationAuxinconcentrationWPM1MBAWPM2MBAMS1MBAMS2MBA§MSN10.40.280.1231/2N6.60.760.1151/4N5.40.680.126NH4+-N/NO3--NNH4+-N/NO3--N2:17.20.8030.1121:18.00.9280.1161:210.41.2800.1231:54.20.6950.165•l12-16hr100-3000lxl100064.3ab4.8b200073.4a5.6a300056.5b3.4clxl••••••12BA••••••••9223253.03817252.5205252.082251.000250.0BA6-BA26.781.443.3301.276.723301.093.328300.83013•••••••••••••••nnAUX••••••••••—••3.23.42.32.92.32.11.61.3--1991••••••1•••••20d30d•••••••§§•••nn0.1%3-5minn••§500-800§1/4-1/2MS§100%90%80%2%50%80%§§§§§§§§§§§§§§§