5种灵芝孢子粉多糖含量的分析对比研究

2009No．3SerialNo．204ChinaBrewingGanodermaLucidum。GanodermaLucidumsporepolysaccharide[1-2]。、、。、、、。5。11.1、5。754-。。1.21.2.1-。1.2.2100mL20mL1.2g。0.1g100mL1.0mg/mL。0mg/mL、0.1mg/mL、0.15mg/mL、0.2mg/mL、0.25mg/mL、0.3mg/mL、0.35mg/mL、0.4mg/mL。1mL5mL30min~35min754-490nm。。1.2.3[4-5]。1g50mL1∶2020mL5min25min140W。5min。2。22.1530038425min70℃。-。3。-O657.32A0254-5071200903-0162-02ContrastivestudyoncontentofGanodermaLucidumpolysaccharidesfromfiveGanodermaLucidumsporesBANLitong,YANGHongpeng,WUJiang(DepartmentofAgronomy,TianjinAgriculturalUniversity,Tianjin300384,China)Abstract:HotwaterextractionassistedwithultrasonicwasappliedtoextractGanodermaLucidumpolysaccharidesfromGanodermaLucidumsporesinthispaper.Theresultsshowedthattheprecisionofthismethodwasfineundertheconditionof25minultrasonicextractionat70℃.Phenol-sulphuricmethodwasappliedtodeterminethecontentofpolysaccharidesinGanodermaLucidum.Theresultsshowedthat3#sampleofGanodermaLucidumsporecontainedmorepolysaccharideswhichhadbetterstability.Keywords:GanodermaLucidumpolysaccharides;phenol-sulphuricmethod;ultrasonicextraction2008-10-281972-。01#2#3#4#5#6#/mg·mL-100.10.150.20.250.30.357#8#0.40.6/mL5050.25350.40550.52150.66050.80150.90751.06851.5561Table1.ResultofstandardcurveAnalysisandExamination162··200932041y=2.515xR2=0.9990。0mg/L~0.6mg/L。2.2222。270℃。[7]。70℃。2.332#、3#4#3#。2.451mL、5%1mL、5mL20min5RSD4。4RSD0.31%~0.87%。2#。2.551mL2h8h。0h～8h。2.61mg/mL2mL1mg/mL8mL0.2mg/mL。0.5mL0.5mL55。5121.4%~87.1%RSD2.08%~3.56%。34.6%[6]。。53。[1].[M].1999./℃6070/mg·mL-1/%0.8630.328612.10.9280.353213.122Table2.Polysaccharidesconcentfrom2#GanodermaLucidumsporeatdifferentultrasoundtemperature35Table3.Polysaccharidescontentof5GanodermaLucidumsporesample1#2#3#4#5#/℃/min/W/mg·mL-1/%70251400.4960.18886.9870251400.9280.353213.170251400.9960.37911470251400.9580.364613.170251400.7640.290811.912345RSD/%1#2#3#4#5#0.4370.9240.9060.9580.7160.4310.9380.9140.9520.7210.4320.9370.9100.9490.7220.4380.9240.9100.9530.7210.4370.9210.9100.9610.7240.740.870.310.510.414Table4.Resultsofprecisionexperiments5Table5.Resultsofaveragerecoveryrate/mg/mg/mg/%RSD/%1#2#3#4#5#0.08320.17580.17240.14920.09820.10.10.10.10.10.18880.2740.29380.25310.1853105.698.2121.4103.987.13.012.083.562.962.35n=5n=5163··2009No．3SerialNo．204ChinaBrewing[2].[J].2002345493-498.[3].[J].2008126-29.[4].-[J].200512112-15.[5].[J].2005306471-472.[6].[J].2007285118-121.[7].[J].2007282190-192.2、、、、、[1]。、、[2]。[3]。。HPLC、、。[4]。、、、。[5-6]。HPLC。11.1150mL3g1L[7]。1.2ShimadzuLC-10ATvpSPD-10AvpCTO-10ASvpC-R8AAutoscienceAP-9901SAutoscienceAS3120BWaterMillipore1/10000050mLChemElut。。1.31.3.1[4]。HibarRTLichrospherC18250×4.0mm5μm0.8mL/min30℃。280nm。A∶98∶2B。0min～8minB12%8min～20minB18%～40%20min～25minB40%～0%。1.3.2-014040。15mLpHpH。O657.7A0254-5071200903-0164-03Solid-phaseextractionandHigh-performanceliquidchromatographydeterminationofpolyphenolsinwineWENGHongzhen,YUETaixin,CHENGYufeng(BaotouLightIndustryVocationalTechnicalCollege,BaoTouCityInnerMongoliaAutonomousRegion,014040)Abstract:Themethodsofsolid-phaseextractionwereoptimisated,thebestconditionsofsamplepreparationusedtodetermrine8polyphenolsinwinebyHPLCwereestablished.TheresultsshowedthatwhenthepHofwinesampleswas3.2,dichloromethanevolumewas15mLandethyletherasor-ganicsolventsthe8polyphenolsinwinewasseparatedsuccessfullywithahighextractionsof8polyphenols.KeywordsSolid-phaseextraction;High-performanceliquid;polyphenols2008-11-121957-、、。AnalysisandExamination!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!164··