不同成熟度杨梅果实采后唿吸速率`乙烯释放速率和品质的变化

417,JournalofPlantPhysiologyandMolecularBiology2005,31(4):4174242005-01-122005-06-17(973)(No.G2000046806)(No.30270917)*(E-mail:[e-mail];Tel:[phone])1234111111*(131002923150003315400431800):3(immature)(mature)(ripe)32048h3hPAL-3-(Cy-3-Glu)CIRGCy-3-Glu(r=0.96**)::Q945(MyricarubraSieb.&Zucc.)20~224d46%27%3d7d100%(1993)(Chen2004)(1994)24~48h(1994;2001)48h(2001;Chen2004)3(immature)(mature)(ripe)33h11.1(MyricarubraSieb.&Zucc)20032004CIRG(ColorIndexforRedGrape)(Carreño1995)(20±0.5)DPSOrigin1.25250mL1h2mLICA40CO2(InternationalControlledAtmosphereLtd.,)CO2Zhang(2003)3h3418311.31--1-(ACC)ACC(ACS)ACC(ACO)ACCACSZhang(2003)ACO(2000)31.4(TSS)(TA)TSS10Moradshahi(1977)TA1.0g1020mL5000×g15min10mL20mmol/LNaOHpH8.2TAmmolH+/g31.5Ding(2002)3.0g103~5min80%3520min4000×g15min325mL3mL(Heidolp)351.0mL5000×g410min(HPLCBeckman)3(/=80/20V/V1mL/minIntertsil(4.6mm×250mm)156(Jasco)50mmol/L(NH4)2HPO4pH2.70.5mL/minBeckmanODSC18(4.6mm×250mm)166UV-Vis210nm1.6CIRGTC-P2A()L*()a*()b*()h=arctanb*/a*C=[(a*)2+(b*)2]0.5Carreño(1995)CIRG=(180−h)/(L*+C)1.7102.0g10mL0.05%-24h3(12h-6h-6h)(Heidolp)401.0mL()45000×g10minEppen-dorf20-3-(Cy-3-Glu)PolyphenolsA.S.HPLC(2004)1.8(PAL)KoukolConn(1961)PAL1.5g107.5mL(200mmol/LpH8.80.001mol/LDTT0.005mol/Lβ-0.001mol/LEDTA)10%PVPP415000×g20minZucker(1965)PAL3.8mL2mL(200mmol/L,pH8.8)1mLL-(50mmol/L)0.8mL3090min200mLHCl(6mol/L,1:1=V/V)290nmODOD0.0122.1200324h6h1.031.84µLkg-1FWh-12042h3(1D)ACCACSACO(1A~C)20032004419:4(2)18h15h0.08mmolkg-1FWh-1120ACCACSACOFig.1ChangesinACCcontent,ACSandACOactivitiesandethyleneproductionrateofChinesebayberryvar.Biqifruitwithdifferentmaturitiesatharvest,andheldat20220Fig.2ChangesinrespirationrateandethyleneproductionrateofChinesebayberryvar.Dongkui&Zaodameifruitwithdifferentmaturitiesatharvest,andheldat20420310.08mmolkg-1FWh-148h0.05mmolkg-1FWh-1(2)2.266hTSS10.9%27.3%29.3%(3)30~36h36h26.1837.2347.59mg/gFW22.3030.2743.86mg/gFW42h(3)TA0.32mmolH+/g66h0.23mmolH+/g28.1TA(4)85.0%~88.0%2066h23.9%46.4%26.6%11.699.596.46mg/gFW320TSSFig.3Changesintotalsolublesolids(TSS)andsolublesugarcontentsofChinesebayberryvar.Biqifruitwithdifferentmaturitiesatharvest,andheldat20421:48.414.855.03mg/gFW66h(4)(1994)Cy-3-GluCIRGCy-3-Glu(r=0.96**)(1)Cy-3-Glu(1)PALPAL420TAFig.4Changesintitratableacidity(TA)andorganicacidcontentsofChinesebayberryvar.“Biqi”fruitwithdifferentmaturitiesatharvest,andheldat20120CIRGPALTable1ChangesinCIRG,anthocyanincontentandPALactivityofChinesebayberryvar.“Biqi”fruitwithdifferentmaturi-tiesatharvest,andheldat20°CImmatureMatureRipeTime(d)CIRGCy-3-GluPALCIRGCy-3-GluPALCIRGCy-3-GluPAL(mg/gFW)(U/mgPro)(mg/gFW)(U/mgPro)(mg/gFW)(U/mgPro)01.89±0.31g0.09±0.01g1.38±0.06f2.70±0.53e0.19±0.01de1.56±0.04c6.67±0.27a0.50±0.01a1.06±0.06g12.26±0.41f0.12±0.01f1.44±0.03e3.06±0.38d0.21±0.01cd1.71±0.03b5.64±0.34b0.45±0.01b1.02±0.04g23.16±0.57d0.17±0.01e1.63±0.04d4.76±1.06c0.23±0.02c1.77±0.01a5.59±0.25b0.44±0.04b0.94±0.02h42231(Wang2000)2048hPAL(1)Cy-3-Glu3(BialeYoung1981)(1994)(2001)215d1~2d3h324h(12)(Chen2004)24~48h(2001;1994)24~48h(1994)()(24~48h)Cy-3-Glu61.2%70.0%70.0%(3)87.43%86.87%85.43%(4)Cy-3-Glu(1)L*a*b*Hunter(Hunter1948)COL=(2000×a*)/(L*×C)(Hobson1987)CCI=(1000×a*)/(L*×b*)(Jimenez-Cuesta1981)CIRG=(180−h)/(L*+C)CCICOL(P0.05)(Carreño1995)CIRGCIRGCy-3-Glu(r=0.96**)(1)CIRG()CIRG45()(2001;1994)BaileJB,YoungRE(1981).Respirationandripeninginfruits:retrospectandprospect.In:FriendJ,RhodesMJC(eds).RecentAdvancesintheBiochemistryofFruitandVegetables.London:AcademicPress,139CarreñoJ,MartinezA,AlmelaL,Fernandez-LopezJA(1995).Proposalofanindexfortheobjectiveevaluationofthecolourofredtablegrapes.FoodResIntl,28:373377ChenKS(),XuCJ(),ZhangB(),Ferguson423:4IB(2004).Redbayberry:botanyandhorticulture.HorticRev,30:83114DingCK,ChachinK,UedaY,ImahoriY,WangCY(2002).Modifiedatmospherepackagingmaintainspostharvestqualityofloquatfruit.PostharvestBiolTechnol,24:341348HobsonGE(1987).Lowtemperatureinjuryandthestorageofripeningtomatoes.JHortSci,62:5562HuXQ(),YuX(),ChenLG()(2001).StudiesonsomephysiologicalcharactersofChinesebayberryfruitduringstorage.JZhejiangUniv(Agric&LifeSci)[()],27:179182(inChinese)HuYD(),JiaHJ(),SunCD(),ChenQJ(),XuCJ(),ChenKS()(2004).Amethodforextractinganddetermininganthocyaninfrompeachfruitanditsapplication.JFruitSci(),21(2):167169(inChinese)HunterRS(1948).Photoelectriccolordifferencemeter.JOptSocAmer,38:661Jimenez-CuestaM,CuquerellaJ,Martinez-JavagaJM(1981).Determinationofacolorindexforcitrusfruitsdecreeing.ProcIntSocCitriculture,2:750753KoukolJ,ConnEE(1961).Themetabolismofaromaticcompoundsinhigherplants.IV.PurificationandpropertiesofthephenylalaninedeaminaseofHordeumvulgare.JBiolChem,236:26922698MoradshahiA,VinesHM,BlackCC(1977).CO2exchangeandaciditylevelsindetachedpineapple,Ananascomosus(L.)Merr.leavesduringthedayatvarioustemperatures,O2andCO2concentrations.PlantPhysiol,59:274278WangHQ,ArakawaO,MotomuraY(2000).Influenceofmaturityandbaggingontherelationshipbetweenanthocyaninaccumulationandphenylalanineammonia-lyase(PAL)activityin‘Jonathan’.PostharvestBiolTechnol,19:123128XiYF(),ZhengYH(),QianDM(),