普通油茶种子4个发育时期的转录组分析

201194498505MolecularPlantBreeding,2011,Vol.9,No.4,498505ALetter41*11121,,311400;2,,321025*,[e-mail]、、、。solexa“4”480310unigene2178924All-unigene27.13%。KEGG42638unigenePathwaypathway265、、pathway。。(Camelliaoleifera),Solexa,Unigene,PathwayTranscriptomeAnalysisofCamelliaoleiferaAbelSeedinFourDevelopmentStagesLinPing1*CaoYongqing1YaoXiaohua1WangKailiang1TengJianhua21ResearchInstituteofSubtropicalForestry,ChineseAcademyofForestry,Fuyang,311400;2DongfanghongForestFarmofZhejiangProvince,Jin-hua,321025*Correspondingauthor,[e-mail]:10.3969/mpb.009.000498AbstractTheoilteaindustryisdevelopingveryfastinChina,andgreatimprovementshavebeenmadeinplantletpropagationandculturetechnology.While,themolecularmechanismsonteaoilproduction,qualityandresistancewerenotresearched,andwhichwillhamperthedevelopmentofoilteaindustrygreatly.ToinvestigatetheprofileofgeneexpressioninCamelliaoleiferaandelucidateitsfunctionalgene,Solexasequencingwereusedtoproduceasubstantialexpressedsequencetagsdatasetfromtheseedof“Changlin4”.Atotalof80310unigenesand21789unigeneswithproteinfunctionannotationsweregenerated.Theunigeneswithfunctionannotationscanbeclassifiedintotwenty-fourfunctional-categoriesandaccountfor27.13%ofallunigenes.TheAll-UnigenewerequeriedagainsttheKEGGpathwaydatabase,and42638unigenesweregiventhepathwayannotationsandrelatedto265pathways,includingmetabolism,geneticinformationprocessingcellmetabolismpathwaysandsoon.DatapresentedinthisstudywillconstituteanimportantresourceforthescientificcommunitythatisinterestedinthemoleculargeneticsandfunctionalgenomicsofCamelliaoleifera.KeywordsCamelliaoleifera,Solexasequencingtechnology,Unigene,Pathway“”(2009BADB1B01;2009BADA8B0406)、(CAFYBB2008005;CAFYBB2008011;CAFYBB2008036)(RISF6804)(Camelliaoleifera)3×106hm2200(,2008)。。MolecularPlantBreeding、、、、、、、、、、。cDNA(,2006;,2008;,2008a;,2008b;,2008a;,2008b;,2009;,2009;Wangetal.,2009;Tanetal.,2009)。、。(Mortazavietal.,2008)。solexa。“DNA”“”()、、(Marionietal.,2008;Hoenetal.,2008;Tangetal.,2009;WilhelmandLandry,2009)。mRNAmRNA(Nagalakshmietal.,2008;Yassouraetal.,2009;Mortazavietal.,2008;Sultanetal.,2008)。、、SmallRNA、Chip-Seq。、。solexa“4”、、。11.1RNA4RNA28S:18S1.8~2.0RNARIN8.3~9.0RNAS189ng/μL(1)RNAsolexa。1.2contig、scaffold480310unigene。All-unigene200bp~3000bp3000bp6400.8%。300bp~1500bpunigene91.1%。All-unigeneGap(N)3801949.3%。N13%89.36%(2)。1.3unigenenr、Swiss-Prot、KEGGCOGUnigene2178924generalfunctionpredictiononly376117.26%。TranscriptionReplication,recom-binationandrepair9.79%9.73%。Nuclearstructure13(3)。1.4unigenepathwayKEGG42638unigenePathwaypathway265(1)。unigene14561、、、1S1RNA:RNA:89ng/μL;rRNA(28s/18s):2.0;RNA(RIN):9.2Figure1RNAdetectionresultofS1stageNote:RNAconcentration:89ng/μL;rRNAratio(28s/18s):2.0;RNAintegritynumber(RIN):9.24994TranscriptomeAnalysisofCamelliaoleiferaAbelSeedinFourDevelopmentStages2All-unigeneGapFigure2LengthandgapdistributionofAll-unigene、、、、、、、、137。12951unigene、、、。unigene7844、、、、、、、、、、47。unigene3277、、27。1.5unigeneGOGeneOntology(GO)(controlledvocabulary)。GOontology()(molecularfunction)、3unigene:A:RNA;B:;C:;D:,,;E:;F:;G:;H:;I:;J:,;K:;L:,;M:/;N:;O:,,;P:;Q:,;R:;S:;T:;U:,;V:;Y:;Z:Figure3FunctionclassificationofunigeneNote:A:RNAprocessingandmodification;B:Chromatinstructureanddynamics;C:Energyproductionandconversion;D:Cellcyclecontrolcelldevision,chromosomepartitioning;E:Aminoacidtransportandmetabolish;F:Nucleotidetransportandmetabolism;G:Carbohydratetransportandmetabolism;H:Coenzymetransportandmetabolism;I:Lipidtransportandmetabolism;J:Translation,ribosomalstructureandbiogenesis;K:Transcription;L:Replication,recombinationandrepair;M:Cellwall/membrane/envelopebiogenesis;N:Cellmotility;O:Posttranslationalmodification,proteinturnover,chaperones;P:Inorganiciontransportandmetabolism;Q:Secondarymetabolitesbiosynthesis,transportandcatabolism;R:Generalfunctionpredictiononly;S:Functionunknown;T:Signaltransductionmechanisms;U:Intracellulartrafficking,secretion,andvesiculartransport;V:Defensemechanisms;Y:Nuclearstructure;Z:Cytoskeleton(cellularcomponent)、(biologicalprocess)。GOterm(、)term。GOGO。30150unigeneGOBiologicalprocessunigene9073cellularcomponent13832molecularfunction7245(4)。2500MolecularPlantBreeding1UnigenepathwayTable1PathwayannotationandclassificationofunigeneUnigeneUnigenenumber349662178764218617711731343618828256541982641417949532460135916174013022707119020089271379598PathwayPathwaynumber34622271232351487943133211593141712BehaviorCellcommunicationCellgrowthanddeathCellmotilityCirculatorysystemDevelopmentEndocrinesystemImmunesystemNervoussystemSensorysystemTransportandcatabolismMembranetransportSignaltransductionSignalingmoleculesandinteraction,Folding,sortinganddegradationReplicationandrepairTranscriptionTranslationAminoacidmetabolismBiosynthesisofpolyketidesandnonribosomalpeptidesBiosynthesisofsecondarymetabolitesCarbohydratemetabolismEnergymetabolismEnzymefamiliesGlycanbiosynthesisandmetabolismLipidmetabolismMetabolismofcofactorsandvitaminsPathwayPathwayannotationCellularprocessesEnvironmentalinformationprocessingGeneticinformationproce