高速逆流色谱及其在天然产物分离中的研究进展

335122221.4710032.471003highspeedcountercurrentchromatographyHSCCC、、－、、、、。。ResearchprogressofapplicationofHSCCCintheseparationandpurificationofnaturalproductsXINLi1SHIJiang2LIUSu－yun2GUOYong－xin2YUFei－yan21.CollegeofFoodandBioengineeringHenanUniversityofScience＆TechnologyLuoyang471003China2.CollegeofAgricultureHenanUniversityofScience＆TechnologyLuoyang471003ChinaAbstractHigh－speedcounter－currentchromatographyHSCCCisakindofliquid－liquidpartitionchromatographywhichhastheuniquefeaturesofhighspeedhighefficiencyandcontinuousprocessingcapability.IthasbeenwidelyusedintheseparationandpurificationofChineseherbalmedicinesthenaturalproductsandfoodaswellasinbiochemistryandinenvironmentalchemistryanalysis.Inthispapertheapplicationandthetechnologyofhigh－speedcounter－currentchromatographyforpurificationoffunctionalingredientsfromnaturalproductswerereviewed.KeywordshighspeedcountercurrentchromatographynaturalproductsseparationsummaryTS201.1A1002－0306200911－0335－05檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾檾199417－52.23TannockGW.Afreshlookattheintestinalmicroflora//ProbioticsACriticalReviewHoriozonScientificPressJ.Wymondham19995－14.24AlvarezOMIOberhelmanRA.ProbioticagentsandinfectiousdiseaseamodemperspectiveonatraditionalthempyJ.ClinInfectDis200132111567－1576.25KoppERuslanMJamesCetal.ECSITisanevolutionarilyconservedintermediateintheTolIILisignaltransductionpathwayJ.GeNesDev1999132059－2071.26RowlandIR.Metabolicinteractionsinthegut//Probiotics.TheScientificBasisRFullerEd.Chapman＆HallLondon199229－54.27AdriananowakZdzislawalibudzisz.Abilityofintestinallacticbacteriatobindor/andmetabolisephenolandp－cresolJ.AnnalsofMicrobiology2007573329－335.high－speedcountercurrentchromatographyHSCCC－。、2008－12－221967－。、。、、、、、、。。13361.11－3———800r/min13。。1.24－5a.。、HSCCC、、。b.。HSCCC。c.。、、、、。HSCCC。d.。90%。e.。1.37－8。、、、、6。。。Ito9、。//////。Ito10///1∶1∶1∶1//10∶3∶7。。。。SFCO2SFCO2、、、。R134a1112－tetrafluoroethane。SFCO2。4。1.4ultravioletdetectorUVfouriertransformationinfraraldetectorFTIRevaporativelightscatteringdetectorELSD。11－13。HSCCCMS。Oka14THSCCCEIMS、CIMS、FABMSESIMS———HSCCC、、。22.1HSCCCHSCCCHSCCC。、、HSCCC。Wei15HSCCCHSCCC－－－1∶1∶1∶1v/v/v/v5∶5∶4.5∶5.5v/v/v/v、5。2.2HSCCC、、、。。Shibusawa16－－－4∶4∶3∶4v/v/v/v、15。3。3373.1Alkaloids－－、－－－。Ling17－－2.3×10－2mol/LNaH2PO427.5∶20∶12.5v/v/v、。Liu18－－0.2mol/L3∶2∶2v/v/v200mg25.6mg10.3mg。Liu19－－－15∶30∶21∶20v/v/v/v200mg8.6mg96.4%。OuYang20－－－6∶4∶5∶8v/v/v/v700mg210mg、90mg、220mg100mg。Tang21－－－0.2mol/L1∶3.5∶2∶4.5v/v/v/v2g10.4mgPGuanfubaseP、9.2mgGGuanfubaseG、9.5mgFGuanfubaseF、8.9mg、11.9mgGuanfubaseA25.7mgIGuanfubaseI。3.2－－－－。Gao22－－－－1∶6∶1.5∶1∶4v/v/v/v/v－－－0.1%5∶2∶5∶10v/v/v/v95.6%。Peng23－－－1∶1.2∶1.2∶1v/v/v/v。Shi24－－1∶1∶2v/v/valquds。Han25－－－7∶10∶7∶10v/v/v/v。Peng26－－－5∶5∶6∶3v/v/v/v。3.3－－－－－－pH。Lu27－TFA－100∶1∶100v/v/v—。N.Kohler28/2－/40∶1∶40v/v/v/2－/20∶1∶20v/v/v/1－/14∶1∶15v/v/v。Gutzeit29－－1∶1∶2v/v/v。Yang30－－－1∶3∶1∶6v/v/v/v。3.4Liu31－－－3∶7∶5∶5v/v/v/v98%。Maier32－－－3∶7∶3∶7v/v/v/v0.5%TFA－－－2∶2∶1∶5v/v/v/v0.5%TFAcaftaricacid、coutaricacidfertaricacid。Shi33－－2∶5∶7v/v/v。3.5Baldermann34－－5∶5∶4.5v/v/v9′－－。Frighetto35－－－10∶5∶2.5∶1v/v/v/v。Shi36－－－0.05%TFA5∶10∶2∶20v/v/v/v。3.6Yao37－－5∶1∶5v/v/v、5∶1∶4v/v/v、5∶2∶5v/v/v60～90℃－－－6∶4∶5∶5v/v/v/vα－、β－10。Ha38－－－6∶6∶4∶1v/v/v/vRg3、Rk1、Rg5F4。Han39－－0.6∶4.4∶5v/v/vBPseudostellarinB。HSCCC、、。HSCCCHSCCC、、。1GUEGUENGGAIGEBGREVYJMetal.Structure－activityanalysisoftheeffectsoflysophophatidicacidonplateletaggregationJ.Biochemistry199938268440－8450.2ItoY.High－speedCountercurrentChromatographyJ.CRCCritRevAnalChem19861765.3HisaoOkaOkaHItoY.Systematicsearchforsuitabletwo－phasesolventsystemforhigh－speedcounter－currentchromatographyJ.Chromatography1991538199－108.4.J.3382001295586－591.5.J.200622299－101.6.J.2007282372－374.7.—J.200616478－80.8.M.200530－60.9ItoY.Efficientpreparativecounter－currentchromatographywithacoilplanetcentrifugeJ.JChromatography1981214122－125.10ItoY.Goldenrulesandpitfallsinselectingoptimumconditionsforhigh－speedcounter－currentchromatographyJ.JChromatographyA20051065145－168.11.J.—20079154－58.12.J.20079524－27.13ZhouTTZhuZYWangCetal.On－linepuritymonitoringinhigh－speedcounter－currentchromatographyApplicationofHSCCC－HPLC－DADforthepreparationof5－HMFneomangiferinandmangiferinfromAnemarrhenaasphodeloidesBungeJ.JPharmaceuticalandBiomedicalAnalysis20074496－100.14OkaFOkaHItoY.Systematicsearchforsuitabletwo－phasesolventsystemsforhigh－speedcountercurrentchromatographyJ.JChromatographyA199153899－108.15YunWeiYoichiroIto.PreparativeisolationofimperatorinoxypeucedaninandisoimperatorinfromtraditionalChineseherb“baizhi”AngelicadahuricaFisch.exHoffmBenth.etHookusingmultidimensionalhigh－speedcounter－currentchromatographyJ.JChromatographyA20061115112－117.16YoichiShibusawaYutakaYamakawaRyokoNojietal.Three－phasesolventsystemsforcomprehensiveseparationofawidevarietyofcompoundsbyhigh－speedcounter－currentchromatographyJ.JChromatographyA20061133119－125.17JianYaLingGuoYingZhangZhaoJieCuietal.SupercriticalfluidextractionofquinolizidinealkaloidsfromSophoraflavescensAit.andpurificationbyhigh－speedcounter－currentchromatographyJ.JChromatographyA2007114