显微操作针制作相关问题的探讨

,3,,(,150081),,,,,piezo,,,;;R319A100423845(2007)0620419204ConsiderationsinthepreparationofmicroneedleJIHong,LIUHui2wen,QINYi2ren,LIUDong2huaDepartmentofHistology&Embryology,HarbinMedicalUniversity,HeilongjiangHarbin150086AbstractThemicroneedleisanessentialtoolinmicromanipulationanditspreparationdirectlyimpactsonthemicromanipulation.Inthispaper,thecleaning,silicification,pullingandforgingofmicroneedle,thecalcinationsofholdingmicropipettecaliber,thesharpeningofmicroinjectionpipette,andsomerelatedinfluentialfactorsandconsiderationsareintroducedformicromanipulatorsexperimentreference.KeywordsMicroneedles;Micromanipulation;Preparation(JReprodMed2007,16(6):4192422)([1][2][3])[4][5][6][7],Leica(Leica,),Piezo(PRIMETECH,),,,,,,,(micromanipulator),,,1.01.5mmNikon(Nikon,)Leica,1.0mm,0.020.15mm,,,,,75%,,:,15min,,,5min,,,1.0mm0.8mm,2007204217;2007206218(LRB2000136),(10551139)(1981),,,,.(3)9142007121661.0mm0.6mm,PN230(Narishige,),,,,Piezo,,Leica,,,,:(heater)(magnetmain)(magnetsub),,,6595;,,65100;,,,(1)1(mm)(m)(m)81020-Piezo1014510-58152080100:,Piezo1.::(1):,,,;(2):MF2900(Narishige,),,50,,,;,,,80m,,,,,30,,3s,(1):,,,;,,:,,;;,,,2.:,50,(2),,,,,;,,,1.:,,Leica20m,Piezo10mPiezo(3),Leica2.:EG2400(Narish2ige,),4550,0,,,50,75%(4),3.:,,25,,,,,(5),,2030Piezo1520:,,024200712166,50,,,,,(6)Leica,Z,,[8][9][10]pH,,,,,,,123456124200712166[1]LieRT,LyngstadaasA,OrstavikKH,etal.BirthdefectsinchildrenconceivedbyICSIcomparedwithchildrenconceivedbyotherIVF2methods;ameta2analysis[J].IntJEpidemiol,2005,34(3):6962701.[2]PatratC,WolfJP,EpelboinS,etal.Pregnancies,growthanddevelopmentofchildrenconceivedbysubzonalinjectionofspermatozoa[J].HumReprod,1999,14(9):240422410.[3]KishigamiS,VanThuanN,HikichiT,etal.Epigeneticabnormalitiesofthemousepaternalzygoticgenomeassociatedwithmicro2inseminationofroundspermatids[J].DevBiol,2006,289(1):1952205.[4]YangX,SmithSL,TianXC,etal.Nuclearreprogrammingofclonedembryosanditsimplicationsfortherapeuticcloning[J].NatGenet,2007,39(3):2952302.[5]KishigamiS,BuiHT,WakayamaS,etal.SuccessfulmousecloningofanoutbredstrainbytrichostatinAtreatmentaftersomaticnucleartransfer[J].JReprodDev,2007,53(1):1652170.[6]LeeGS,KimHS,LeeSH,etal.Successfulsurgicalcorrec2tionofanalatresiainatransgenicclonedpiglet[J].JVetSci,2005,6(3):2432245.[7]LallemandY,RrulelP.Aninsituassessmentoftheroutesandextentsofcolonisationofthemouseembryobyembryonicstemcellsandtheirdescendants[J].Development,1990,110(4):124121248.[8]ZhouQ,YangSH,DingCH,etal.Acomparativeapproachtosomaticcellnucleartransferintherhesusmonkey[J].HumReprod,2006,21(10):256422571.[9]HeindryckxB,VanderElstJ,DhontM.Culturemediumpreferencesofpre2implantationclonedmouseembryos[J].MethodsMolBiol,2006,348(36):59278.[10]BhakJS,LeeSL,OckSA,MohanaKumarB,etal.Develop2mentalrateandploidyofembryosproducedbynucleartrans2ferwithdifferentactivationtreatmentsincattle[J].AnimReprodSci,2006,92(122):37249.(uFSH2HP),(HMG)(hCG)10,,,,,(uFSH2HP),,,,FSH2FSH,20,Metrodin2HP,,,::1.:60,2.,4,000,5003.,,:200841():,:1.:2.:3.:4.5.,6.:,()2008,,:[e-mail]:075628135316,8135935,E2mail:[e-mail]224200712166