桔梗提取工艺及质量控制的研究进展

1*230039ResearchAdvancesinExtractionProcessandQualityControlofPlatycodongrandiflorumA.DCCHENChenCHENQin(SchooloflifeScience,AnhuiUniversity,AnhuiKeyLaboratoryofChineseMedicineResearchandDevelopment,Hefei230039)AbstractThispaperresearchonextractionandidentificationofactiveingredientinthePlatycodongrandiflorum,andbriefstheseveralmethodsofextractionprocessandqualitycontrol,andcomparedwitheachmethod’sadvantagesandproperlyapplicablerangesbasedontherecentresearchresults.KeywordsPlatycodongrandiflorum;activeingredient;extractionprocess;qualitycontrolPlatycodongrandiflorum(Jacq.)A.DC.[1][2][3]95%NO.081035701(1958)2E-mail:[e-mail]40[4]platycodin11.1[1]1g7%-1320ml3h20ml30ml1.2-1.2.1[5]8g50ml1.5h1050.37g4.22%1.2.2[5]8g50ml501.5h0.44g5.43%1.2.3-[6]5g50ml4030min20ml50ml10ml5ml1/350ml605h0.21g4.2%58.9%3-1.3[5]8g50ml95%1.5h1050.53g6.42%[7]5kg95%24h4803[8]70%351h1.41.4.1-[9]2070%536h46h448hZTC-120%30%40%50%95%2.05%1.4.2-[10]-80.5h620.5h95%1.603%1.5[11]MAS-1/3g24%4mm=12020%600W110s80-10060D3.87mg/g1.6CO2[12]100g4530MPa4h4035MPa4h100ml/100g22.1[1]4g25ml15h25ml6h12h1520ml50ml20ml10ml5ml1520ml50ml1052.2[4]-D-AB-81ml706015min10min5ml475nm96.8%[13]D--99.22%RSD=1.8%-D5r=0.9997D33%2h16230min25ml20µl10ml0.5ml10%60%5ml6015min3min478nm2.32001[14]-DD1.2mg10ml20µlDC185µl4.6mm250mm=28720.7ml/min0.32.4µgr=0.999199.57%[15]DD0.4mg/ml0.45µm10µlC18250mm×4.6mm252%2857151.0ml/min210nm25DD[16]D150µl/ml0.45µm0.02mol/L0.7ml/min206nmD0.01500.1496mgr=0.9999100.91%[17]EE0.4mg/ml,0.45µmwatersnova-pakC18(3.9mm×300mm4µm)PDA190400nm1121.0ml/min20µlE121.8974.4mg/Lr=0.9997,98.7%6EDEE3[1].()[S].2005.,2005,196.[2],,.[J].,2006,23(3):184-191.[3],.[J]..2006,6:58-59.[4],,.[J].2008,25(1):58-60.[5].[J]..2007,28(4):133-134.[6],,,.-[J].(),2006,20(2):135-137.[7],,.[J].,2007,28(20):33-34.[8],,.[J].,2002,25(6):430-432.[9],,,.[J].,2001,23(2):50-52.[10],,,.-[J].(),2002,23(1):15-17.[11],,,.D[J]..2008,25(11):1070-1074.[12],,,.CO2[J].(),2007,28(1):43-45.[13],,,.[J]..2006,24(12):2232-2234.[14],,,.HPLC-ELSDD[J].,2001,10(4):11-13.[15],,,.HPLCD[J].7,2007,31(6):501-503.[16],,.RP-HPLCD[J].,2006,26(2):283-284.[17],,.HPLC-ELSDE[J].,2006,26(5):637-639.200912